Effects of lactic acid bacteria and molasses on fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro ruminal fermentation of rice straw silage

Abstract

Objective This study was to evaluate the fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro gas production of rice straw ensiled with lactic acid bacteria and molasses. Methods Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (C), Lactobacillus plantarum (L), molasses (M) and molasses+ Lactobacillus plantarum (ML) for 6, 15, 30, and 60 days. After storage, the silages were subjected to microbial and chemical analyses as well as the further in vitro fermentation trial. Results All additives increased lactic acid concentration, and reduced pH, dry matter (DM) loss and structural carbohydrate content relative to the control (p<0.05). The highest organic acid and residual sugar contents and lignocellulose reduction were observed in ML silage. L silage had the highest V-score with 88.10 followed by ML silage. L and ML silage improved in vitro DM digestibility as compared with other treatments, while in vitro neutral detergent fibre degradability (IVNDFD) was increased in M and ML silage (p<0.05). M silage significantly (p<0.05) increased propionic acid (PA) content and decreased butyric acid content and acetic acid/PA as well as 72-h cumulative gas production. Conclusion The application of ML was effective for improving both the fermentation quality and in vitro digestibility of rice straw silage. Inclusion with molasses to rice straw could reduce in vitro ruminal gas production.

Effects of Rice Straw Silage with Combining Additives of Lactobacillus Plantarum, Trichoderma Viride and Wheat Bran on the Growth Performance, Digestibility and Rumen Fermentation in Growing Lambs

“…However, cellulase or cellulolytic enzymes or bacteria could promote the degradation of crude fiber to dietary degradable carbohydrates such as glucose and organic acid (Tamboli et al, 2017). Therefore, RSSA supplementation enhanced the nutrients digestibility, and similar to this in vivo study, the application of bacterial inoculant also increased nutrients digestibility of rice straw silage in vitro (Zhao et al, 2019). Thereby, ruminal microorganisms can easily degrade the carbohydrate to produce volatile fatty acids, which promote the nutrient digestibility.…”

Co-ensiling garlic stalk with citrus pulp improves the fermentation quality and feed-nutritional value

“…In support, Huisden et al [19] reported that the addition of molasses to corn silage supplied an extra source of WSC for LAB metabolism, which possibly allowed their domination in the microbial community of the silage, thereby stimulating favorable silage fermentation patterns. Earlier findings confirmed that the combined use of the LAB inoculant and molasses improved the silage quality indices of various crops [7,8,22]. For example, our recent investigation [8] found that the combination of LAB and 5% molasses efficiently improved the silage quality parameters of spent mushroom substrate in both laboratory-scale and ton-bag silos.…”

Effect of storage time and the level of formic acid on fermentation characteristics, epiphytic microflora, carbohydrate components and in vitro digestibility of rice straw silage

“…It cannot be neglected that most straw is ensiled with high fermentation losses even if the fermentation accelerant is added. In our previous study [2], high ammonia nitrogen (NH 3 -N) and ethanol content were produced not only in control but also in rice straw en-siled with molasses or enzyme and lactic acid bacteria (LAB). Considering the relatively low protein and water soluble carbohydrates (WSC) content in rice straw, it seems particularly important to reduce protein and substrate losses during ensiling.…”