Effects of Lactobacillus Johnsonii AJ5 Metabolites on Nutrition, Nosema Ceranae Development and Performance of Apis Mellifera L.

Piano, Fiorella G. De; Maggi, Matías; Pellegrini, Maria; Cugnata, Noelia Melina; Szawarski, Nicolás; Buffa, Franco; Negri, Pedro; Fuselli, Sandra R.; Audisio, Carina; Ruffinengo, Sergio

doi:10.1515/jas-2017-0007

Cited by 8 publications

(9 citation statements)

References 56 publications

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“…A recent field study by De Piano et al (66) showed that honeybee colony performance did not change after administration of Lactobacillus johnsonii AJ5 metabolites. On the other hand, in another field study, administration of a monoculture of Bacillus subtilis subsp.…”

Section: Discussionmentioning

confidence: 99%

Honeybee-Specific Lactic Acid Bacterium Supplements Have No Effect on American Foulbrood-Infected Honeybee Colonies

Stephan

Lamei

Pettis

et al. 2019

Appl Environ Microbiol

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Paenibacillus larvae, the causative agent of American foulbrood (AFB), is the primary bacterial pathogen affecting honeybees and beekeeping. The main methods for controlling AFB are incineration of diseased colonies or prophylactic antibiotic treatment (e.g., with tylosin), neither of which is fully satisfactory. The search for superior means for controlling AFB has led to an increased interest in the natural relationships between the honeybee-pathogenic and mutualistic microorganisms and, in particular, the antagonistic effects of honeybee-specific lactic acid bacteria (hbs-LAB) against P. larvae. These effects have been demonstrated only on individual larvae in controlled laboratory bioassays. Here we investigated whether supplemental administration of hbs-LAB had a similar beneficial effect on P. larvae infection at colony level. We compared experimentally AFB-infected colonies treated with hbs-LAB supplements to untreated and tylosin-treated colonies and recorded AFB symptoms, bacterial spore levels, and two measures of colony health. To account for the complexity of a bee colony, we focused on (Bayesian) probabilities and magnitudes of effect sizes. Tylosin reduced AFB disease symptoms but also had a negative effect on colony strength. The tylosin treatment did not, however, affect P. larvae spore levels and might therefore “mask” the potential for disease. hbs-LAB tended to reduce brood size in the short term but was unlikely to affect AFB symptoms or spores. These results do not contradict demonstrated antagonistic effects of hbs-LAB against P. larvae at the individual bee level but rather suggest that supplementary administration of hbs-LAB may not be the most effective way to harness these beneficial effects at the colony level. IMPORTANCE The previously demonstrated antagonistic effects of honeybee-derived bacterial microbiota on the infectivity and pathogenicity of P. larvae in laboratory bioassays have identified a possible new approach to AFB control. However, honeybee colonies are complex superorganisms where social immune defenses play a major role in resistance against disease at the colony level. Few studies have investigated the effect of beneficial microorganisms on bee diseases at the colony level. Effects observed at the individual bee level do not necessarily translate into similar effects at the colony level. This study partially fills this gap by showing that, unlike at the individual level, hbs-LAB supplements did not affect AFB symptoms at the colony level. The inference is that the mechanisms regulating the honeybee microbial dynamics within a colony are too strong to manipulate positively through supplemental feeding of live hbs-LAB and that new potential remedies identified through laboratory research have to be tested thoroughly in situ, in colonies.

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Section: Discussionmentioning

confidence: 99%

Honeybee-Specific Lactic Acid Bacterium Supplements Have No Effect on American Foulbrood-Infected Honeybee Colonies

Stephan

Lamei

Pettis

et al. 2019

Appl Environ Microbiol

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“…The three species of Lactobacillus isolated from beebread for the first in Jujuy, formed a monophyletic clade separate from bee intestinal strains isolated by other authors in Salta. These last had exhibit beneficial effect to honeybees [4,5,8,11,13].…”

Section: Discussionmentioning

confidence: 99%

Effect of Lactic Bacteria on <i>Ascosphaera apis</i> and <i>A. atra </i>

Tejerina¹,

Cabana²,

Carrillo³

et al. 2018

AJAFS

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The lactic bacteria contribute to fermentation of corbiculae pollen and beebread production. Three species of Lactobacillus, isolated from beebread for the first time in Jujuy (Argentine), formed a monophyletic clade separate from others bee intestinal strains. Lactic bacteria isolated in this work affected the growth and sporulation of Ascosphaera apis and Ascosphaera atra, and they can provide to beehive a protection against ascospores.

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“…Commercial probiotic preparation also based on lactic acid bacteria were found to be effective against the same parasite [27,36]. Similarly, De Piano et al [39] demonstrated a strong relation between bacterial metabolites and the count of N. ceranae spores, showing a significant decrease after Lactobacillus johnsonii AJ5 administration. Additionally, organic acids produced by lactic acid bacteria present in the honey bee's environment (flowers, beebread, and gut), such as lactic acid, acetic acid, and phenyl-lactic acid, were tested, through feeding, against these microsporidia and showed a strong reduction in spore load in bees [38].…”

Section: Introductionmentioning

confidence: 87%

Screening of Dietary Ingredients against the Honey Bee Parasite Nosema ceranae

et al. 2021

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Nosema ceranae is a major pathogen in the beekeeping sector, responsible for nosemosis. This disease is hard to manage since its symptomatology is masked until a strong collapse of the colony population occurs. Conversely, no medicaments are available in the market to counteract nosemosis, and only a few feed additives, with claimed antifungal action, are available. New solutions are strongly required, especially based on natural methods alternative to veterinary drugs that might develop resistance or strongly pollute honey bees and the environment. This study aims at investigating the nosemosis antiparasitic potential of some plant extracts, microbial fermentation products, organic acids, food chain waste products, bacteriocins, and fungi. Honey bees were singularly infected with 5 × 104 freshly prepared N. ceranae spores, reared in cages and fed ad libitum with sugar syrup solution containing the active ingredient. N. ceranae in the gut of honey bees was estimated using qPCR. The results showed that some of the ingredients administered, such as acetic acid at high concentration, p-coumaric acid, and Saccharomyces sp. strain KIA1, were effective in the control of nosemosis. On the other hand, wine acetic acid strongly increased the N. ceranae amount. This study investigates the possibility of using compounds such as organic acids or biological agents including those at the base of the circular economy, i.e., wine waste production, in order to improve honeybee health.

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Effects of Lactobacillus Johnsonii AJ5 Metabolites on Nutrition, Nosema Ceranae Development and Performance of Apis Mellifera L.

Cited by 8 publications

References 56 publications

Honeybee-Specific Lactic Acid Bacterium Supplements Have No Effect on American Foulbrood-Infected Honeybee Colonies

Honeybee-Specific Lactic Acid Bacterium Supplements Have No Effect on American Foulbrood-Infected Honeybee Colonies

Effect of Lactic Bacteria on <i>Ascosphaera apis</i> and <i>A. atra </i>

Screening of Dietary Ingredients against the Honey Bee Parasite Nosema ceranae

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