Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Abstract

Studies on the health-promoting effects of lactic acid bacteria (LAB) are numerous, but few provide examples of the relationship between LAB function and culture conditions. We verified the effect of differences in culture conditions on Lactobacillus plantarum OLL2712 functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. We measured interleukin-10 (IL-10) and IL-12 production in murine immune cells treated with OLL2712 cells prepared under various culture conditions. The results showed that the IL-10-inducing activities of OLL2712 cells on murine immune cells differed dramatically between OLL2712 groups at different culture phases and using different culture medium components, temperatures, and neutralizing pHs. In particular, exponential-phase cells had much more IL-10-inducing activity than stationary-phase cells. We confirmed that the Toll-like receptor 2 (TLR2) stimulation activity of OLL2712 cells depended on culture conditions in conjunction with IL-10-inducing activity. We also demonstrated functional differences by culture phases in vivo; OLL2712 cells at exponential phase had more anti-inflammatory activity and anti-metabolic-disorder effects on obese and diabetic mice than those by their stationary-phase counterparts. These results suggest that culture conditions affect the functionality of antiinflammatory LAB.IMPORTANCE While previous studies demonstrated that culture conditions affected the immunomodulatory properties of lactic acid bacteria (LAB), few have comprehensively investigated the relationship between culture conditions and LAB functionality. In this study, we demonstrated several culture conditions of Lactobacillus plantarum OLL2712 for higher anti-inflammatory activity. We also showed that culture conditions concretely influenced the health-promoting functions of OLL2712 in vivo, particularly against metabolic disorders. Further, we characterized a novel mechanism by which changing LAB culture conditions affected immunomodulatory properties. Our results suggest that culture condition optimization is important for the production of LAB with anti-inflammatory activity.KEYWORDS culture phase, dendritic cell, interleukin-10, interleukin-12, Lactobacillus plantarum, macrophage, metabolic disorder, mono-oleic acid ester, proinflammatory cytokine, Toll-like receptor L actic acid bacteria (LAB) have been reported to modulate immune activities and prevent or ameliorate immune-related disorders, including inflammatory bowel diseases, allergies, and certain metabolic disorders (1-3). Certain LAB strains have been selected as the most suitable strains against each disorder (4-6). In addition to the

Metabolic Variability of a Multispecies Probiotic Preparation Impacts on the Anti-inflammatory Activity

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“…Indeed, previous studies have shown that oleic acid affected the cell growth (Endo et al, 2006 ) or survival in the gastric juice (Corcoran et al, 2007 ) of some lactobacilli strains by being incorporated into bacterial cell surface membrane lipids. Toshimitsu et al ( 2017 ) have reported that decaglycerol mono-oleic acid 1 ester Q-17S, normally used as an emulsifier for food, affected the immunomodulatory properties of L. plantarum strains, while the same effects were not observed with decaglycerol mono-stearic acid ester Q-18S, highlighting the fact that subtle changes in culture media deeply impact on structure of bacterial cell components and functionality.…”

“…The fact that culture conditions, among other factors, impact on strain functionality is widely recognized. The IL-10-inducing activity of L. plantarum OLL2712 differs dramatically according to different growth phases, culture medium components, temperatures, and pH (Grangette et al, 2005 ; Toshimitsu et al, 2017 ). The exponential phase cells display enhanced IL-10-inducing activity in comparison to stationary phase cells, while the toll-like receptor (TLR)-2 stimulatory activity depends on media components and culture conditions.…”

Beneficial Microbes: Roles in the Era of Antimicrobial Resistance

“…being an integral part of the intestinal microflora having earned the "Generally Regarded as Safe" status are the most successful probiotic candidates. Lactic acid producing bacteria are known to possess various health benefits such as anti-cancer activity, lowering of serum cholesterol, lactose intolerance alleviation, prevention of antibiotic related diarrhea, stimulation of the immune functions, antimicrobial activity against resistant pathogens [8,9] prevention and treatment of Inflammatory bowel disease [10], respiratory viral infection [11]. Recently, Lactobacillus spp.…”

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