Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

Abstract

Probiotics have been suggested as a prophylactic measure in colon cancer. The aim of this study was to investigate the impact of Lactobacillus salivarius Ren (Ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (DMH). The results indicated that oral administration of Ren could effectively suppress DMH-induced colonic carcinogenesis. A significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed with a dose of 5 × 10(10) CFU/kg bodyweight per day. Using denaturing gradient gel electrophoresis and Real-time PCR combined with multivariate statistical methods, we demonstrated that injection with DMH significantly altered the rat gut microbiota, while Ren counteracted these DMH-induced adverse effects and promoted reversion of the gut microbiota close to the healthy state. Tvalue biplots followed by band sequencing identified 21 bacterial strains as critical variables affected by DMH and Ren. Injection of DMH significantly increased the amount of Ruminococcus species (sp.) and Clostridiales bacteria, as well as decreasing the Prevotella sp. Administration of Ren reduced the amount of Ruminococcus sp., Clostridiales bacteria, and Bacteroides dorei, and increased the amount of Prevotella. Real-time PCR results were consistent with the results derived by t-value biplots. These findings suggested that Ren is a potential agent for colon cancer prevention. In conclusion, the results in the present study suggest a potential therapeutic approach based on the modulation of intestinal microflora by probiotics may be beneficial in the prevention of colorectal carcinogenesis.

Applications and safety considerations ofLactobacillus salivariusas a probiotic in animal and human health

“…These findings suggested that Ren is a potential agent for colon cancer prevention (Zhang et al . ). Lastly, Zhu et al .…”

“…Administration of Ren reduced the amount of Ruminococcus, Clostridiales bacteria and Bacteroides dorei, and increased the amount of Prevotella. These findings suggested that Ren is a potential agent for colon cancer prevention (Zhang et al 2015). Lastly, Zhu et al (2014) found that Ren prevents early colorectal carcinogenesis in a DMHinduced rat model.…”

Isolation and Probiotic Potential of Lactobacillus Salivarius and Pediococcus Pentosaceus in Specific Pathogen Free Chickens

“…These results showed a lot of different indicators of the effects of probiotics on meat quality (Suo et al, 2012). Zhang et al (2015) considered that the shear force of raw drumstick decreased in the whole yeast treatment relative to the controls. Zhou et al (2010) demonstrated that B. coagulans probiotic affected meat quality by decreasing the drip loss of broiler breast meats of Guangxi Yellow chickens.…”

“…Increasing evidence indicates that Lactobacillus and Pediococcus strains exert beneficial effects by a variety of complementary mechanisms, including an ability to modulate host immune function, high adhesion capacity, cancerpreventing attributes, reduction of serum cholesterol levels, high antimicrobial activity against pathogens, and normalization of the microbiota composition (Okamoto et al, 2007;Noujaim et al, 2009; Isolation and Probiotic Potential of Lactobacillus salivarius and Pediococcus pentosaceus in Specific Pathogen Free Chickens Meng et al, 2010;Zhang et al, 2011;Kiran et al, 2015;Xie et al, 2015;Zhang et al, 2015). Promising probiotic strains should have some superior biological properties, especially improving growth performance, intestinal microbiota balance, and meat quality, and decreasing noxious gas emission (Meng et al, 2010;Fan et al, 2013;Jeong et al, 2014;Peng et al, 2016).…”

“…Pediococcus pentosaceus and Lactobacillus salivarius are most frequently isolated from the duodenal mucosa of piglets (Zhang et al, 2011;Zhang et al, 2015) and human breast milk (Osmanagaoglu et al, 2010;Kiran et al, 2015), but little information is available about the isolated strains from animals and their bioavailability. Therefore, the objectives of this study were to isolate and identify P. pentosaceus and L. salivarius strains from cecal content and investigate their potential probiotic effects on growth, meat quality and microenvironment in specific pathogen free (SPF) chickens, and ammonia treatment in vitro.…”