Effects of laser‐modified polystyrene substrate on CHO cell growth and alignment

Zhu, Bangshang; Lu, Qi; Yin, Jie; Hu, Jun; Wang, Zongguang

doi:10.1002/jbm.b.30011

Cited by 40 publications

(28 citation statements)

References 21 publications

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“…1,[4][5][6][7][8][9][10][11][12] A nanostructured surface can be used to control cell differentiation and, thereby, the performance of biomaterials after implantation. [13][14][15][16] Here, we report the cellular behavior on a silicon surface, structured on the cm 2 scale by femtosecond laser ablation with high spatial frequency laser-induced periodic surfaces structures (HSF-LIPSS or HSFLs). [17][18][19][20][21] Note that silicon serves as a model surface for fundamental studies and not as typically used biomaterial.…”

Section: Introductionmentioning

confidence: 99%

Cellular reactions toward nanostructured silicon surfaces created by laser ablation

Wallat

Dörr

Harzic

et al. 2012

Journal of Laser Applications

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Silicon wafers were structured with a femtosecond laser on the cm 2 scale with high spatial frequency laser-induced periodic surface structures. These areas are characterized by regular parallel ripples with a period of the order of 100 nm. The particular ripple spacing is determined by the illumination wavelength of the tunable femtosecond laser. The cellular reaction to the structured silicon wafers and to the same materials, coated with calcium phosphate nanoparticles by electrophoretic deposition, was studied using L929 fibroblasts, human mesenchymal stem cells, and epithelial cells. The cells adhered uniformly to structured and unprocessed areas after seeding but significantly preferred the unstructured silicon after 48 h. This behavior disappeared after coating the structured surface with calcium phosphate nanoparticles. V C 2012 Laser Institute of America.

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Section: Introductionmentioning

confidence: 99%

Cellular reactions toward nanostructured silicon surfaces created by laser ablation

Wallat

Dörr

Harzic

et al. 2012

Journal of Laser Applications

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“…This agrees with previous work on CHO cells by Rebollar et al [55], who reported the alignment of CHO cells with a periodic surface nanostructure. CHO cells were chosen due to the fact that they are well studied, easy to culture, and so is an ideal cell type [56][57] for initial experiments on a novel substrate. We are currently working on other cell types, including primary cells, on our substrates.…”

Section: Introductionmentioning

confidence: 99%

Aligned, isotropic and patterned carbon nanotube substrates that control the growth and alignment of Chinese hamster ovary cells

et al. 2011

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Here we culture Chinese Hamster Ovary cells on isotropic, aligned and patterned substrates based on multiwall carbon nanotubes. The nanotubes provide the substrate with nanoscale topography. The cells adhere to and grow on all substrates, and on the aligned substrate, the cells align strongly with the axis of the bundles of the multiwall nanotubes. This control over cell alignment is required for tissue engineering; almost all tissues consist of oriented cells. The aligned substrates are made using straightforward physical chemistry techniques from forests of multiwall nanotubes, no lithography is required to make inexpensive largescale substrates with highly aligned nanoscale grooves. Interestingly, although the cells strongly align with the nanoscale grooves, only a few also elongate along this axis: alignment of the cells does not require a pronounced change in morphology of the cell. We also pattern the nanotube bundles over lengthscales comparable to the cell size and show that the cells follow this pattern.3

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“…The surface properties of an implanted biomaterial are of the utmost importance for its biocompatibility. As for a biomaterial surface, the cell-material interaction is strongly influenced by not only surface topography but also by surface chemistry (physicochemical property) including surface wettability (surface energy) and surface charge [1][2][3][4][5] .…”

Section: Introductionmentioning

confidence: 99%

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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Surface wettability is an important physicochemical property of biomaterials, and it would be more helpful for understanding this property if a wide range of wettability are employed. This study focused on the effect of surface wettability on fibroblast adhesion over a wide range of wettability using a single material without changing surface topography. Plasma polymerization with hexa methyldisiloxane followed by oxygen (O 2 ) plasma treatment was employed to modify the surfaces. The water contact angle of sample surfaces varied from 106 degrees (hydrophobicity) to almost 0 degrees (super-hydrophilicity). O 2 -functional groups were introduced on polymer surfaces during O 2 -plasma treatment. The cell attachment study confirmed that the more hydrophilic the surface, the more fibroblasts adhered in the initial stage that includes on super-hydrophilic surfaces. Cells spread much more widely on the hydrophilic surfaces than on the hydrophobic surfaces. There was no significant difference in fibroblast proliferation, but cell spreading was much greater on the hydrophilic surfaces. These findings suggest the importance of the surface wettability of biomaterials on initial cell attachment and spreading. The degree of wettability should be taken into account when a new biomaterial is to be employed. Further research of surface wettability on adhesive molecules is necessary for a better understanding of this property.

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Effects of laser‐modified polystyrene substrate on CHO cell growth and alignment

Cited by 40 publications

References 21 publications

Cellular reactions toward nanostructured silicon surfaces created by laser ablation

Cellular reactions toward nanostructured silicon surfaces created by laser ablation

Aligned, isotropic and patterned carbon nanotube substrates that control the growth and alignment of Chinese hamster ovary cells

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

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