Effects of laser polar-body biopsy on embryo quality

Levin, Ishai; Almog, Benny; Shwartz, Tamar; Gold, Veronica; Ben‐Yosef, Dalit; Shaubi, Michal; Amit, Ami; Malcov, Mira

doi:10.1016/j.fertnstert.2012.02.008

Cited by 44 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hammoud and co-workers further investigated the effect of using lasers for polar body biopsies and concluded that there were no deleterious effects of laser drilling for polar body biopsy in in-vitro matured oocytes in terms of oocyte lysis, oocyte activation or chromosome breakage in embryos (Hammoud et al, 2010). In another study, laser-treated groups showed significantly higher chemical pregnancy rates, clinical pregnancy rates and live birth rates compared with controls (Levin et al, 2012). FIGURE 2 shows a typical polar body biopsy.…”

Section: Laser-assisted Polar Body Biopsymentioning

confidence: 91%

Laser technology in the ART laboratory: a narrative review

Davidson

Liu

Griffiths

et al. 2019

Reproductive BioMedicine Online

View full text Add to dashboard Cite

This review discusses the current status of laser-assisted reproductive technologies in human infertility treatment. We highlight the benefits of laser technology in diagnostic and therapeutic treatments for subfertility and male-factor infertility, and current concern of this application in different clinical practice. ABSTRACTTo improve success rates, assisted reproductive technology (ART) procedures continually undergo optimization and enhancement such that the best quality gametes and embryos can be identified and manipulated, thus improving clinical outcomes. Laser technology is now being applied across ART to reduce procedure times and increase the consistency and reproducibility of traditional ART techniques such as assisted hatching, embryo biopsy, intracytoplasmic sperm injection cryopreservation and sperm immobilization/selection. This review examines the current status of cutting-edge laser-assisted reproductive technologies, investigates experimental techniques that are increasingly being applied clinically. It highlights the benefits of lasers as a powerful technology at the forefront of both diagnostic and therapeutic treatments for general subfertility and male-factor infertility. However, it is important to note that although lasers are becoming increasingly commonplace in ART units, there is comparatively little information in the existing literature pertaining to the potential negative effects that laser application might have on the developing human embryo, thus creating the need for further investigative research.

show abstract

Section: Laser-assisted Polar Body Biopsymentioning

confidence: 91%

Laser technology in the ART laboratory: a narrative review

Davidson

Liu

Griffiths

et al. 2019

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…This turns to be disadvantaging compared to the diagnosis of blastomeres or trophectoderm biopsy where the embryo genetic status is directly diagnosed instead of being deduced. Yet, ADO events can jeopardize the results and frequently an additional biopsy of the embryos is required, which turns the PB diagnosis into highly complex and exhausting for IVF and PGD labs [28]. After practicing this approach for several years at our unit, it has been decided not to opt for it unless it is inevitable (for example with de novo maternal mutations).…”

Section: Discussionmentioning

confidence: 99%

Improving preimplantation genetic diagnosis (PGD) reliability by selection of sperm donor with the most informative haplotype

Malcov

Gold

Peleg

et al. 2017

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

BackgroundThe study is aimed to describe a novel strategy that increases the accuracy and reliability of PGD in patients using sperm donation by pre-selecting the donor whose haplotype does not overlap the carrier’s one.MethodsA panel of 4–9 informative polymorphic markers, flanking the mutation in carriers of autosomal dominant/X-linked disorders, was tested in DNA of sperm donors before PGD. Whenever the lengths of donors’ repeats overlapped those of the women, additional donors’ DNA samples were analyzed. The donor that demonstrated the minimal overlapping with the patient was selected for IVF.ResultsIn 8 out of 17 carriers the markers of the initially chosen donors overlapped the patients’ alleles and 2–8 additional sperm donors for each patient were haplotyped. The selection of additional sperm donors increased the number of informative markers and reduced misdiagnosis risk from 6.00% ± 7.48 to 0.48% ±0.68. The PGD results were confirmed and no misdiagnosis was detected.ConclusionsOur study demonstrates that pre-selecting a sperm donor whose haplotype has minimal overlapping with the female’s haplotype, is critical for reducing the misdiagnosis risk and ensuring a reliable PGD. This strategy may contribute to prevent the transmission of affected IVF-PGD embryos using a simple and economical procedure.Trial registrationAll procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. DNA testing of donors was approved by the institutional Helsinki committee (registration number 319-08TLV, 2008). The present study was approved by the institutional Helsinki committee (registration number 0385-13TLV, 2013).

show abstract

“…From a basic science viewpoint, there are several issues to be resolved. First, whether biopsy of the polar body, early blastomeres or trophectoderm may in itself have negative short-term or long-term effects on the offspring as well as being inaccurate [29,30] Second, what is the impact of mitotic errors and instability on early human development [31] and lastly whether, as suggested for large animal embryos, abnormal cells may be tolerated by the human embryo, sequestered into the trophectoderm or indeed eliminated by cell death mechanisms [32]. Finally, we would like to point out that, in any case, perfect euploidy is not a guarantee for development and that embryos may also block in development due to non-genomic effects and probably single gene defects.…”

Section: Clinical Concernsmentioning

confidence: 99%

Trends, Fads and ART!

Dale¹,

Ménézo²,

Coppola³

2015

J Assist Reprod Genet

View full text Add to dashboard Cite

Morphological selection techniques of gametes and embryos are of current interest to clinical practice in ART. Although intracytoplasmic morphologically selected sperm injection (IMSI), time lapse imaging morphometry (TLIM) or quantification of chromosome numbers (PGS) are potentially useful in research, they have not been shown to be of statistically predictive value and, thus, have only limited clinical usefulness. We make the point that morphological markers alone cannot predict the success of the early embryo, which depends on the correct orchestration of a myriad of physiological and biochemical activation events that progress independently of the maternal or zygotic genome. Since previous attempts to identify metabolic markers for embryo quality have failed and there is no evidence that the intrinsic nature of gametes and embryos can be improved in the laboratory, embryologists can only minimize environmental or operator induced damage while these cells are manipulated ex vivo.

show abstract

Effects of laser polar-body biopsy on embryo quality

Cited by 44 publications

References 16 publications

Laser technology in the ART laboratory: a narrative review

Laser technology in the ART laboratory: a narrative review

Improving preimplantation genetic diagnosis (PGD) reliability by selection of sperm donor with the most informative haplotype

Trends, Fads and ART!

Contact Info

Product

Resources

About