The gear transmission system is advancing towards high-speed and heavy-duty applications. Among the main failure modes of the system, tooth surface scuffing due to increased tooth surface temperature has emerged as a prominent concern in mechanical transmission. Addressing the enhancement of gear scuffing resistance has thus become an urgent challenge in this field. This paper utilized discrete laser surface melting (DLSM) treatment to create discrete laser surface melted (DLSMed) units on the surface of W6Mo5Cr4V2 steel gears, resembling the radial ribs found on the surface of Limaria basilica. The paper investigated the size, hardness, residual austenite content, and residual stress of the DLSMed units at varying current intensities and laser frequencies. Microstructural observations were conducted on the DLSMed units, followed by gear scuffing experiments performed on the Forschungsstelle für Zahnräder und Getriebebau (FZG) testing machine. The experimental findings revealed that the change in laser frequency had a clearly weaker impact on the size of the DLSMed unit compared to current intensity. The DLSMed unit consisted of two parts: the melting zone (MZ) and the heat-affected zone (HAZ), with equiaxed and dendritic microstructures, respectively. Both zones exhibited refinement with increasing current intensity and laser frequency. Moreover, the microhardness of the DLSMed unit showed significant improvement compared to that of as-received gears. The scuffing resistance of DLSMed gears was found to be closely linked to their initial surface roughness. Residual stress formation in DLSMed gears was attributed to thermal stress and microstructural stress. The distribution pattern of DLSMed units had varying effects on the scuffing load-carrying capacity of DLSMed gears. Specifically, DLSMed gears with transverse distribution of DLSMed units demonstrated a 12.5% improvement in anti-scuffing performance compared to those with longitudinal distribution. Finally, this paper elucidated the mechanism through which DLSM enhances the scuffing resistance of W6Mo5Cr4V2 steel gears.