Effects of Lead on the Activity of δ-Aminolevulinic Acid Dehydratase in Gammarus pulex

Kutlu, Hatice Mehtap; Sümer, Sibel

doi:10.1007/s001289900700

Cited by 7 publications

(3 citation statements)

References 7 publications

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“…Sassa (1982) reported that ALAD from rats showed maximum activity at 55 • C. In Channel catfish, ALAD was found to be heat resistant and reached the maximal activity at 65 • C (Conner and Fowler 1984). In contrast, Gammarus pulex ALAD was reported to be heat sensitive and its maximal activity temperature was 37 • C (Kutlu and Sümer 1998). In our study, it was observed that ALAD has an optimal temperature at 30 • C. * p < 0.05 (the effects of metals on ALAD are statistically significant) a One hundred micrograms of extracted protein was exposed to 500 μM of metal salts b The values are based on three experimental replicates, and the numbers in parentheses represent standard deviation (SD) ALAD proteins from diverse organisms require either zinc or magnesium for activity, but the role of the latter metal has not been described.…”

Section: Resultsmentioning

confidence: 91%

δ-Aminolevulinic acid dehydratase of Haloarcula argentinensis isolated from Tuz Lake in Turkey

Korcan

Mutlu

Çiğerci

et al. 2009

Environ Monit Assess

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The delta-aminolevulinic acid dehydratase (ALAD) enzyme of a novel record for Turkish microbial flora was studied. The isolate I-113 was obtained from Tuz Lake in Turkey and identified as Haloarcula argentinensis. The ALAD enzyme of the isolate was assayed in order to determine its requirements and to be used as biomarker for lead pollution in it's ambient. In enzymic studies, the effects of various metals (Cd, Co, Mg, Mn, Ni, Pb, and Zn), pH (3-11), temperatures (25-55 degrees C), and salinity (15-25%) conditions have been examined. The data obtained from the studies were analyzed statistically by using Kruskal-Wallis, Mann-Whitney, correlation, regression, variance analysis, and significance tests were performed by using SPSS 10.0 for Windows. Although its optimum pH was determined as 7, it was still active at pH 3-11. The optimal temperature for the enzyme was observed to be 30 degrees C. Mn and Pb inhibited its activity significantly (p < 0.05) while Zn increased it slightly. The ALAD enzyme in H. argentinensis could be used as a biomarker for Pb contamination.

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Section: Resultsmentioning

confidence: 91%

δ-Aminolevulinic acid dehydratase of Haloarcula argentinensis isolated from Tuz Lake in Turkey

Korcan

Mutlu

Çiğerci

et al. 2009

Environ Monit Assess

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“…Among heavy metals, lead is a ubiquitous environmental contaminant and belongs to the group of most toxic heavy metal in the biosphere. It is considered as a non-specific poison affecting physiological systems and can cause damage to central nervous system, kidney, gastrointestinal distress and reproductive disorders (Kutlu and Sumer, 1998). Toxic effect of Lead and other heavy metals on gill histopathology have been mostly investigated in fishes (Gill et al, 1988;Khangarot and Tripathi, 1990;Kumari and Kumar, 1995;Parashar andBanerjee, 2002 andOlojo et al, 2005;Mobarak and Sharaf, 2011).…”

Section: Introductionmentioning

confidence: 99%

Histopathological alterations in gills of freshwater prawn, Macrobrachium dayanum (Crustacea - Decapoda) after acute and sub-acute exposure of lead nitrate

Shukla¹,

Tiwari

Lodhi³

et al. 2019

JANS

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Lead is a nonessential “grey listed” heavy metal, used in fuels, ceramics, paints and glass wares in industries and vehicles. After taking entry in aquatic ecosystem it becomes toxic and cause serious problem to plants and animals. Haematological, neurological, nephrological and histopathological effects of lead are well known. Fresh water prawn, Macrobrachium dayanum, a potential animal for freshwater aquaculture, subjected to acute and sub-acute concentration of lead nitrate (116.46 mg/l; 96h LC50 and 29.12 mg/l; 25% of 96h LC50) showed severe histopathological alterations in gills after 24, 48, 72 and 96h and 10, 20 and 30 day exposure. Chief alterations during acute exposure were thickening of gill plates, reduction in inter lameller spaces, cuticular irruption, inflammation and necrosis where as clumping of gill plates, hyperplasia, hypertrophied haemocytes with nuclear pycnosis and leakage of haemocytes in inter-lameller spaces as well as deposition of broken tissue debris near gill base were observed during sub-acute exposure. The severity of histopathological alterations was found duration dependant. Present study reveals that histological bio- markers provides complete information regarding heavy metal toxicity particularly lead to these economically important fresh water prawns, which can themselves serve as bio-indicator of worsening status of surface aquatic bodies.

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“…Lead enters into water bodies from industries and smelter discharges or dissolution of old lead plumbin (Moore and Rammamoorthy, 1984;Gupta and Salunke, 1985;De, 1996;Satake et al, 1997). Lead is considered as a non-specific poison affecting physiological systems and can cause brain damage, kidney damage, gastrointestinal distress and reproductive disorders (Campana et al, 2003;Kutlu and Summer, 1998). Toxic effect of lead and other heavy metals on opercular beat and oxygen consumption has been mostly investigated in fishes (Hiltbran, 1971;Singh and Singh, 1979;Rao and Ramamurthi, 1987;Gill et al, 1988) but crustaceans despite being important member of food chain and having high economic and medicinal value ar e being documented less in reference to metal toxicity and oxygen consumption (Chinnaya, 1971;Ghate and Mulhelker, 1979;Papathanasiou and King, 1983;Tulasi and Rao, 1989;Reddy and Venugopal, 1993;Chinni et al, 2000;Jadhav and Ambore, 2007;Sen et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effects of lead nitrate on oxygen consumption of fresh water prawn, Macrobrachium dayanum (Crustacea - Decapoda)

Tiwari¹,

Lodhi²,

Shukla³

et al. 2009

ECJ

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Freshwater prawn, Macrobrachium dayanum were subjected to acute concentration, 116.46 mg/l (96 hr LC50 value) and sub-acute concentration, 29.12 mg/l (25% of 96 hr LC50 value) of lead nitrate to evaluate its effects on oxygen consumption. Initial increase in oxygen consumption was noticed which was followed by gradual decrease up to 96 hr in acute exposure while significant (F = 20.33; P < 0.001) decrease in oxygen consumption was noticed through out the experiment during sub-acute exposure up to 30 days. Mechanism of lead intoxication and potential of oxygen consumption as bio-marker has been discussed.

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Effects of Lead on the Activity of δ-Aminolevulinic Acid Dehydratase in Gammarus pulex

Cited by 7 publications

References 7 publications

δ-Aminolevulinic acid dehydratase of Haloarcula argentinensis isolated from Tuz Lake in Turkey

δ-Aminolevulinic acid dehydratase of Haloarcula argentinensis isolated from Tuz Lake in Turkey

Histopathological alterations in gills of freshwater prawn, Macrobrachium dayanum (Crustacea - Decapoda) after acute and sub-acute exposure of lead nitrate

Effects of lead nitrate on oxygen consumption of fresh water prawn, Macrobrachium dayanum (Crustacea - Decapoda)

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