Effects of lead (PB) and cadmium (CD) elements on lipid peroxidation, catalase enzyme activity and catalase gene expression profile in tomato plants

AYDIN, SOYDAM; BÜYÜK, Semra;; İlker;, İlker;; Büyük, Esra; Burcu, Burcu; CANSARAN-DUMAN, İrfan;; ARAS, Demet;; Sümer, Sümer

doi:10.1501/tarimbil_0000001412

Cited by 8 publications

(5 citation statements)

References 34 publications

(48 reference statements)

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“…The expression of cat gene increased in E. aediculatus when exposed to LC50 dose of Cd and Cu after 24 h but started to decrease after 48 h of metal exposure. Similar to this study, earlier reports have mentioned that the expression of cat gene increased in the presence of heavy metal stress in various organisms, but at higher concentrations of heavy metals, decrease in the gene expression has been observed (Aydin et al 2016;Azpilicueta et al 2008;Radhakrishnan 2008;Roh et al 2006).…”

Section: Discussionsupporting

confidence: 89%

Molecular characterization and transcriptional modulation of stress-responsive genes under heavy metal stress in freshwater ciliate, Euplotes aediculatus

et al. 2022

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The concentration of heavy metals in the environment is increasing predominantly due to anthropogenic activities such as mining and other industrial activities. Exposure to metals above a certain threshold level induces deleterious effects in the living organisms. To survive such harsh environment, microbes possess a range of tolerance mechanisms and express stress-responsive genes and/or antioxidant enzymes to detoxify the metal stress.Protozoans, especially ciliates, are highly sensitive to the environmental changes, thereby making them suitable model systems for ecotoxicological studies. Thus, in the present work, the effect of heavy metals such as cadmium and copper has been studied in the freshwater ciliate, Euplotes aediculatus. This study focuses on the activity of antioxidant enzymes namely catalase and glutathione peroxidase in E. aediculatus under the heavy metal stress. Also, the expression of stress-responsive genes; heat-shock protein 70 (hsp70) and catalase (cat), has been studied after metal exposure. It was observed that the enzyme activity and the expression of these genes increased with an increase in the metal concentration and with the duration of metal exposure. Besides, these genes have been characterized to understand their role in cell defense. These genes of fresh water ciliate, therefore, can be used as molecular biomarkers to evaluate heavy metal toxicity.

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Section: Discussionsupporting

confidence: 89%

Molecular characterization and transcriptional modulation of stress-responsive genes under heavy metal stress in freshwater ciliate, Euplotes aediculatus

et al. 2022

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“…The concentrations of heavy metals in this study were selected based on the average of reported values of Cd and Pb in agricultural lands. 8,17,18 ZnONPs at 100 mg L −1 has been used in several previous studies without showing any phytotoxic effects on plant seedlings. 7 During the exposure period, the Hoagland solution was avoided due to the high ionic strength of the nutrient solution that induces aggregation of the ZnONPs.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…When the transpiration rate reached 10 mL/day (after 10 days), the plants were transferred from the Hoagland solution to new tubes, which were filled with solutions containing 100 mg L –1 Pb 2+ + 1.0 mg L –1 Cd 2+ and/or 100 mg L –1 ZnONPs in DI water. The concentrations of heavy metals in this study were selected based on the average of reported values of Cd and Pb in agricultural lands. ,, ZnONPs at 100 mg L –1 has been used in several previous studies without showing any phytotoxic effects on plant seedlings . During the exposure period, the Hoagland solution was avoided due to the high ionic strength of the nutrient solution that induces aggregation of the ZnONPs.…”

Section: Methodsmentioning

confidence: 99%

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Zinc Oxide Nanoparticles Alleviated the Bioavailability of Cadmium and Lead and Changed the Uptake of Iron in Hydroponically Grown Lettuce (Lactuca sativa L. var. Longifolia)

Sharifan

Moore

et al. 2019

ACS Sustainable Chem. Eng.

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Leafy vegetables are a rich source of iron and fibers for the human diet, which may become hazardous if exposed to heavy metal contamination. Cadmium (Cd) and lead (Pb) are two highly toxic metals even at trace concentrations. Engineered nanoparticles (ENPs) can alter the uptake of heavy metals and localization of essential minerals such as iron (Fe) through different mechanisms. The goal of this study was to understand the mutual effects of zinc oxide nanoparticles (ZnONPs) and coexisting heavy metals Pb 2+ and Cd 2+ on their uptake and accumulation as well as their effects on Fe concentrations in romaine lettuce (Lactuca sativa L. var. Longifolia) in a hydroponic system. At termination, shoots were gently separated from the roots, and the concentrations of Pb, Cd, Fe, and Zn in all plant tissues were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). In addition, microbial density analysis in the growth media was performed for each treatment. The results indicated active interactions between ZnONPs and coexisting divalent heavy metals. ZnONPs significantly reduced the accumulation of Cd and Pb in roots by 49% and 81%, respectively. In shoots, Cd was reduced by 30%, and Pb elevated by 44%. Fe concentration in shoots was strongly affected by ZnONPs, and the total Zn in shoots was negatively correspond with the microbial population in the growth media. Exposure to ZnONPs alone increased the total Fe in shoots by 80% compared to controls, and the copresence of ZnONPs and heavy metals increased Fe concentration by about 77%. The results revealed the role of ENPs in governing the uptake and translocation of some essential elements and toxic heavy metals in plants.

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“…With the aging of tissues, as well as with a decrease in their viability, the activity of this enzyme naturally decreases. Also, the importance of catalase functioning lies in the adaptation of plants to environmental conditions [ 73 ]. The potato plants used in the experiments were quite young, and when AGr-Cu is added, new environmental conditions are created to which the plant organism needs to adapt.…”

Section: Discussionmentioning

confidence: 99%

Copper-Containing Bionanocomposites Based on Natural Raw Arabinogalactan as Effective Vegetation Stimulators and Agents against Phytopathogens

Khutsishvili,

Perfileva,

Kon’kova

et al. 2024

Polymers

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Novel copper-containing bionanocomposites based on the natural raw arabinogalactan have been obtained as universal effective agents against phytopathogen Clavibacter sepedonicus and development stimulants of agricultural plants. Thus, the use of such nanosystems offers a solution to the tasks set in biotechnology while maintaining high environmental standards using non-toxic, biocompatible, and biodegradable natural biopolymers. The physicochemical characteristics of nanocomposites were determined using a number of analytical methods (elemental analysis, transmission electron microscopy and spectroscopic parameters of electron paramagnetic resonance, UV–visible, etc.). The results of the study under the influence of the nanocomposites on the germination of soybean seeds (Glycine max L.) and the vegetation of potatoes (Solanum tuberosum L.) showed the best results in terms of biometric indicators. It is especially worth noting the pronounced influence of the nanocomposite on the development of the root system, and the increase in the mass of the potato root system reached 19%. It is also worth noting that the nanocomposites showed a stimulating effect on the antioxidant system and did not have a negative effect on the content of pigments in potato tissues. Moreover, the resulting bionanocomposite showed a pronounced antibacterial effect against the phytopathogenic bacterium. During the co-incubation of phytopathogen Clavibacter sepedonicus in the presence of the nanocomposite, the number of cells in the bacterial suspension decreased by up to 40% compared to that in the control, and a 10% decrease in the dehydrogenase activity of cells was also detected.

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Effects of lead (PB) and cadmium (CD) elements on lipid peroxidation, catalase enzyme activity and catalase gene expression profile in tomato plants

Cited by 8 publications

References 34 publications

Molecular characterization and transcriptional modulation of stress-responsive genes under heavy metal stress in freshwater ciliate, Euplotes aediculatus

Molecular characterization and transcriptional modulation of stress-responsive genes under heavy metal stress in freshwater ciliate, Euplotes aediculatus

Zinc Oxide Nanoparticles Alleviated the Bioavailability of Cadmium and Lead and Changed the Uptake of Iron in Hydroponically Grown Lettuce (Lactuca sativa L. var. Longifolia)

Copper-Containing Bionanocomposites Based on Natural Raw Arabinogalactan as Effective Vegetation Stimulators and Agents against Phytopathogens

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