Effects of Lentinus edodes mycelia on Dietary-Induced Atherosclerotic Involvement in Rabbit Aorta.

Abstract

Lentinus edodes mycelia lowers cholesterol levels and acts as an immunomodulator and tumor-inhibitor in animal models. Lentinus edodes mycelia contains eritadenine (C(9)H(11)O(4)N(5)) and glucans among other biological compounds. However, whether or not Lentinus edodes mycelia is anti-atherogenic remains unknown. We examined the effect of Lentinus edodes mycelia (L.E.M) on atherosclerosis in a rabbit model. Thirty-two Japanese white male rabbits were fed with 1.0% cholesterol for 8 weeks, then divided into groups and given 1) 1.0% cholesterol for over 8 weeks (control), 2) 1.0% cholesterol and 1.0% L.E.M for over 8 weeks, 3) 1.0% cholesterol and 2.0% L.E.M for over 8 weeks, and 4) 1.0% cholesterol and 4.0% L.E.M for over 8 weeks (n=8 each group). Total cholesterol (TC) was measured periodically throughout the experiment. After the experimental periods, the aortas were removed and atherosclerotic lesions were examined histologically, immunohistochemically and morphometrically to determine surface involvement (SI) and an atherosclerotic index (AI). Body weight and TC did not significantly differ among the four groups. Decreases in SI were significant in the 1% L.E.M (26.2+/-10.8%) and 2% L.E.M (29.3+/-15.7%) groups compared with the control (48.7+/-15.3%; p < 0.05). The AI was significantly decreased in the 1% L.E.M (6.62+/-4.31) and 2% L.E.M (7.49+/-3.49) groups compared with the control (16.96+/-9.21; p < 0.05). Foam cells aggregated in thickened intima of dietary-induced atherosclerotic lesions in the rabbit aorta. In contrast, the numbers of foam cells in the intima decreased in the experimental group. No-cholesterol-lowering action or dose-dependant effects of L.E.M were determined in this study, but atherosclerotic development was significantly inhibited, indicating that L.E.M had anti-atherogenic properties. L.E.M may inhibit atherosclerotic development in rabbit aorta and be beneficial as a nutritional supplement.

Difference between Deep Seawater and Surface Seawater in the Preventive Effect of Atherosclerosis

“…Surface involvement was measured according to the following formula. 21) Surface involvement (%)ϭSudan IV positive lesions area/Aortas total surface area ϫ100.…”

“…Atherosclerotic lesions were examined histologically after staining with oil red stain. 21) Statistical Analysis Significant differences between groups and within groups were compared with a one-way ANOVA. When a significant difference was accepted between groups, it was authorized using an unpaired t-test (two tail).…”

Hepatoprotective Effect of Syringic Acid and Vanillic Acid on Concanavalin A-Induced Liver Injury

“…[3][4][5] The mycelia of L. edodes can be cultured in solid medium, and the extract obtained by hot-water treatment (L.E.M.) is commercially available as a nutritional supplement.…”

Hepatoprotective Effect of Extracts from Lentinus edodes Mycelia on Dimethylnitrosamine-Induced Liver Injury

“…Oxidative stress stimulates the activation of HSCs, and substances with antioxidative activity, such as vitamin E, 3) glutathione, 4) and L-cysteine, 5) inhibit HSC activation, thus suppressing liver fibrosis. Recently, many antioxidants have been found in plants including tea, fruit, and vegetables.6) The edible mushroom Lentinus edodes (shiitake) contains several compounds with physiologic activity, such as immunopotentiation, 7) antiatherogenesis, 8) and an anti-HIV effect. 9) Mycelia of L. edodes can be cultured in a solid medium and the extract (L.E.M.)…”

“…6) The edible mushroom Lentinus edodes (shiitake) contains several compounds with physiologic activity, such as immunopotentiation, 7) antiatherogenesis, 8) and an anti-HIV effect. 9) Mycelia of L. edodes can be cultured in a solid medium and the extract (L.E.M.)…”