Effects of lesions in different nuclei of the amygdala on conditioned taste aversion

Molero‐Chamizo, Andrés; Rivera-Urbina, Guadalupe Nathzidy

doi:10.1007/s00221-017-5078-1

Cited by 13 publications

(8 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well established that BLA neurons play a necessary role in CTA learning. Multiple studies confirm that activity in the BLA is required for memory formation and retrieval (Yasoshima et al, 2000; Ferreira et al, 2005; Garcia-de la Torre et al, 2014; Molero-Chamizo, et al, 2017). It is also known, that CTA requires protein synthesis in the BLA (Josselyn et al, 2004), but whether new transcription is also required, and if so, the identities of the required transcripts and the cellular processes they promote were not previously known.…”

Section: Discussionmentioning

confidence: 91%

Deletion of Stk11 and Fos in mouse BLA projection neurons alters intrinsic excitability and impairs formation of long-term aversive memory

Levitan

Liu

Lin

et al. 2019

Preprint

View full text Add to dashboard Cite

27Conditioned taste aversion (CTA) is a form of one-trial learning dependent on basolateral 28 amygdala projection neurons (BLApn). Its underlying cellular and molecular mechanisms 29 are poorly understood, however. We used RNAseq from BLApn to identify learning-30 related changes in Stk11, a kinase with well-studied roles in growth, metabolism and 31 development, but not previously implicated in learning. Deletion of Stk11 restricted to 32 BLApn completely blocks memory when occurring prior to training, but not following it, 33 despite altering neither BLApn-dependent encoding of taste palatability in gustatory 34 cortex, nor transcriptional activation of BLApn during training. Deletion of Stk11 in BLApn 35 also increases their intrinsic excitability. Conversely, BLApn activated by CTA to express 36 the immediate early gene Fos had reduced excitability. BLApn knockout of Fos also 37 increased excitability and impaired learning. These data suggest that Stk11 and Fos 38 expression play key roles in CTA long-term memory formation, perhaps by modulating 39 the intrinsic excitability of BLApn. (149 words) 40 41 103 104 Results 105CTA long-term memory requires BLA transcription 106 In order to determine whether CTA requires new RNA transcription within the BLA, we 107 inhibited transcription by injecting Actinomycin-D (1 µl, 50 ng, bilaterally, Figure 1), a 108 widely used RNA polymerase 2 inhibitor (Alberini, 2009), into the BLA 20 min prior to CTA 109 training, and tested memory 48 hours later. As a control, a separate group of mice 110 received vehicle injection (1 µl of PBS, bilaterally). CTA training consisted of 30 min of 111 access to 0.5% saccharin followed by an intraperitoneal injection of 0.15M LiCl, 2% body 112 weight; (Figure 1-figure supplement 1). A two-way ANOVA comparing vehicle and 113 actinomycin-D injected mice before and after training revealed significant training and 114 129 analysis revealed significant reduction in the consumption of saccharin (CTA vs. Test) for 130 the vehicle group, indicating impairment of learning for the actinomycin-D treated group 131 compared to control mice. As a convergent measure, we also assessed the strength of 132 CTA memory by calculating the relative consumption of saccharin during the test day to 133 that consumed on the training day (Neseliler et al., 2011). The differences between the 134 groups were large (23% in the vehicle group vs. 80 % for the actinomycin D group) and 135 significant. Meanwhile, actinomycin-treated mice were neither impaired in their ability to 136 detect the palatability of saccharin, nor in their drinking behavior-consumption of 137 saccharin during CTA training was similar for the two groups, as was consumption of 138 water 8 hours after the test ( Figure 1E), suggesting that these nonspecific effects cannot 139 account for the memory impairment. Thus, BLA transcription is essential for CTA memory 140 formation. These results extend prior work showing the importance of BLA protein 141 synthesis for CTA memory (Josselyn et al., 2...

show abstract

Section: Discussionmentioning

confidence: 91%

Deletion of Stk11 and Fos in mouse BLA projection neurons alters intrinsic excitability and impairs formation of long-term aversive memory

Levitan

Liu

Lin

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…It is well established that BLA neurons play a necessary role in CTA learning. Multiple studies confirm that activity in the BLA is required for memory formation and retrieval (Yasoshima et al, 2000;Ferreira et al, 2005;Garcia-de la Torre et al, 2014;Molero-Chamizo, et al, 2017). CTA also requires protein synthesis in the BLA (Josselyn et al, 2004), but whether new transcription is also required, and if so, the identities of the required transcripts and the cellular processes they promote were not previously known.…”

Section: Bla Projection Neurons Undergo Transcription Important For Cmentioning

confidence: 99%

“…Although multiple brain regions, including the brainstem, amygdala and the cortex, participate in various aspects of taste behavior (reviewed in Carleton et al, 2010 ), prior work suggests that the basolateral amygdala (BLA) plays a critical role in CTA memory. Disrupting neuronal activity within the BLA blocks the formation and retrieval of CTA memory ( Yasoshima et al, 2006 ; Ferreira et al, 2005 ; Garcia-Delatorre et al, 2014 ; Molero-Chamizo and Rivera-Urbina, 2017 ). This may reflect the fact that BLA projection neurons (BLApn) provide the principal output pathway from the amygdala to forebrain structures including the gustatory cortex and the central amygdala ( Duvarci and Pare, 2014 ) enabling it to distribute taste valance information to these regions ( Piette et al, 2012 ; Samuelsen et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Deletion of Stk11 and Fos in mouse BLA projection neurons alters intrinsic excitability and impairs formation of long-term aversive memory

Levitan

Liu

Yang

et al. 2020

eLife

View full text Add to dashboard Cite

Conditioned taste aversion (CTA) is a form of one-trial learning dependent on basolateral amygdala projection neurons (BLApn). Its underlying cellular and molecular mechanisms remain poorly understood. RNAseq from BLApn identified changes in multiple candidate learning-related transcripts including the expected immediate early gene Fos and Stk11, a master kinase of the AMP-related kinase pathway with important roles in growth, metabolism and development, but not previously implicated in learning. Deletion of Stk11 in BLApn blocked memory prior to training, but not following it and increased neuronal excitability. Conversely, BLApn had reduced excitability following CTA. BLApn knockout of a second learning-related gene, Fos, also increased excitability and impaired learning. Independently increasing BLApn excitability chemogenetically during CTA also impaired memory. STK11 and C-FOS activation were independent of one another. These data suggest key roles for Stk11 and Fos in CTA long-term memory formation, dependent at least partly through convergent action on BLApn intrinsic excitability.

show abstract

“…Pharmacological, lesion, and electrophysiological experiments in alert rodents provide evidence of BLA's role in processing hedonic information in GC (Schafe et al, 1998;Escobar and Bermúdez-Rattoni, 2000;Escobar et al, 2002;Ferreira et al, 2005;Reilly and Bornovalova, 2005;St Andre and Reilly, 2007;Grossman et al, 2008;Fontanini et al, 2009;Piette et al, 2012;Samuelsen et al, 2012;Molero-Chamizo and Rivera-Urbina, 2017). Pharmacological silencing of BLA affects taste-evoked firing rates of GC neurons (Piette et al, 2012;Samuelsen et al, 2012).…”

Section: Significance For Palatability Processing In Gcmentioning

confidence: 99%

Synaptic Integration of Thalamic and Limbic Inputs in Rodent Gustatory Cortex

Stone

Fontanini

Maffei

2020

eNeuro

View full text Add to dashboard Cite

Neurons in the gustatory cortex (GC) process multiple aspects of a tasting experience, encoding not only the physiochemical identity of tastes, but also their anticipation and hedonic value. Information pertaining to these stimulus features is relayed to GC via the gustatory thalamus (VPMpc) and basolateral amygdala (BLA). It is not known whether these inputs drive separate groups of neurons, thus activating separate channels of information, or are integrated by neurons that receive both afferents. Here, we used anterograde labeling and in vivo intracellular recordings in anesthetized rats to assess the potential convergence of BLA and VPMpc inputs in GC, and to investigate the dynamics of integration of these inputs. We report substantial anatomic overlap of BLA and VPMpc axonal fields across GC, and identify a population of GC neurons receiving converging BLA and VPMpc inputs. Our data show that BLA modulates the gain of VPMpc-evoked responses in a timedependent fashion and that this modulation is dependent on the recruitment of synaptic inhibition by both BLA and VPMpc. Our results suggest that BLA shapes cortical processing of thalamic inputs by dynamically gating the excitatory/inhibitory balance of the GC circuit.

show abstract

Effects of lesions in different nuclei of the amygdala on conditioned taste aversion

Cited by 13 publications

References 58 publications

Deletion of Stk11 and Fos in mouse BLA projection neurons alters intrinsic excitability and impairs formation of long-term aversive memory

Deletion of Stk11 and Fos in mouse BLA projection neurons alters intrinsic excitability and impairs formation of long-term aversive memory

Deletion of Stk11 and Fos in mouse BLA projection neurons alters intrinsic excitability and impairs formation of long-term aversive memory

Synaptic Integration of Thalamic and Limbic Inputs in Rodent Gustatory Cortex

Contact Info

Product

Resources

About