Effects of Leucaena leucocephala (Lamk.) Shoot Tips Plus Young Leaf Extract Containing Mimosine on Reproductive System of Male Rats

Burawat, Jaturon; Uabandit, Nongnut; Arun, Supatcharee; Nualkaew, Somsak; Iamsaard, Sitthichai

doi:10.4067/s0717-95022018000301062

Cited by 13 publications

(13 citation statements)

References 28 publications

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“…These proteins have also been found to be localised in Leydig cells, Sertoli cells, spermatogonia, spermatocytes and spermatids, which are assumed to be important for spermatogenesis and androgen synthesis (Chaichun, Arun, Burawat, Kanla, & Iamsaard, ). The presence of TyrPho proteins in rat epididymal tissue and fluid has also been investigated (Sawatpanich et al, ), and improvements in testicular TyrPho protein changes have been associated with normal sperm and testosterone production (Burawat, Uabundit, Arun, Nualkaew, & Iamsaard, ; Iamsaard et al, , ; Iamsaard, Sukhorum, Sampannang, et al, ; Maneenin et al, ; Sampannang et al, ). Changes in TyrPho protein expression in the testis have been associated with stress‐related and diabetic conditions (Arun, Burawat, Sukhorum, Sampannang, Maneenin, et al, ; Arun, Burawat, Sukhorum, Sampannang, Uabundit, et al, ; Sampannang, Arun, Burawat, Sukhorum, & Iamsaard, ; Sampannang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Changes in TyrPho protein expression in the testis have been associated with stress‐related and diabetic conditions (Arun, Burawat, Sukhorum, Sampannang, Maneenin, et al, ; Arun, Burawat, Sukhorum, Sampannang, Uabundit, et al, ; Sampannang, Arun, Burawat, Sukhorum, & Iamsaard, ; Sampannang et al, ). Moreover, some anticancer drugs, such as VPA, methotrexate, ketoconazole and mimosine, have been shown to alter the patterns of TyrPho proteins in the testis and epididymis (Burawat et al, ; Iamsaard et al, , , ; Maneenin et al, ; Sawatpanich et al, ; Sukhorum & Iamsaard, ). Although the toxic effects of VPA on some male reproductive organs (especially the testis and epididymis) have been demonstrated, its effect on the seminal vesicle, the biggest accessory organ that produces essential seminal plasma nutrients, has never been reported.…”

Section: Introductionmentioning

confidence: 99%

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Valproic acid changes the expression of tyrosine‐phosphorylated proteins in rat seminal vesicle

et al. 2019

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Previous studies reported the effects of valproic acid (VPA) on tyrosine‐phosphorylated (TyrPho) protein expression in the testis and epididymis, but its effects on that in the seminal vesicle (SV) have never been demonstrated. This study attempted to investigate the expressions of TyrPho proteins in SV treated with VPA. Sixteen rats were divided into control and VPA‐treated groups. The control rats were injected with normal saline, whereas the treated animals were intraperitoneally injected with VPA (500 mg/kg BW) for 10 consecutive days (n = 8/each). The biochemical parameters in blood plasma and SV fluid were analysed. The SV tissues and fluid were investigated for the presence and expression of TyrPho proteins, androgen receptor (AR) proteins and heat shock protein 70 (Hsp70). Significantly, VPA caused SV atrophy and reductions in secretion and biochemical parameter levels. There were significant increases in many TyrPho proteins in the plasma (a 95 kDa) and SV tissue (a 40 kDa) of the VPA rats. However, the expressions of seminal AR, Hsp70 and TyrPho proteins (50 and 48 kDa) were significantly lower in VPA rats. Recent results have indicated that VPA affected SV morphology and decreased biochemical fluid substances including TyrPho proteins associated with decreased expressions of AR and Hsp70.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Valproic acid changes the expression of tyrosine‐phosphorylated proteins in rat seminal vesicle

et al. 2019

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“…By colorimetric determination, mimosine level in LL leaves grown in North, Thailand was 4.4 % dry weight of total proteins (Chanchay & Poosaran). Our research group has investigated the level of mimosine content in aqueous crude extract of LL shoot tips plus young leaves cultured in Northeastern, Thailand by using Thin Layer Chromatography with method validation for the first time and we found that its mimosine level was 17.35 % dry crude extract (Burawat, 2017). Previous study has demonstrated that LL crude extract could damage male reproductive system (Burawat et al).…”

Section: Discussionmentioning

confidence: 87%

Acute Effects of Mimosine Purified from Leucaena leucocephala on Male Reproductive System of Adult Mice

Kanla¹,

Burawat²,

Arun³

et al. 2018

Int. J. Morphol.

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KANLA, P.; BURAWAT, J.; ARUN, S.; SAWATPANICH, T.; CHAICHUN, A. & IAMSAARD, S. Acute effects of mimosine purified from Leucaena leucocephala on male reproductive system of adult mice. Int. J. Morphol., 36(2):507-512, 2018. SUMMARY. This study attempted to examine the acute effect of purified minosine extracted from Leucaena leucocephala on male reproductive system. Adults male mice were divided into 4 groups (n =8); control and 3 experimental groups treated with purified mimosine at different doses of 15, 30, and 60 mg/KgBW, respectively for 7 consecutive days. The morphological features and weights of body and reproductive organs including testis, epididymis plus vas deferens, and seminal vesicle were compared among groups. In addition, epididymal sperm concentration and the changes of histopathology of testicular tissues in all groups were observed. The results showed that mimosine in all doses did not affect mice body weights. However, all doses of mimosine could significantly reduce the absolute and relative weights of testis and seminal vesicle but not of epididymis plus vas deferens. Significantly, mimosine at doses of 30, and 60 mg/KgBw could decrease sperm concentration. Moreover, the seminiferous atrophy and degeneration were obviously found in mimosine treated mice as compared to the control. In conclusion, consumption of Leucaena leucocephala edible parts containing mimosine could damage male reproductive organs which may cause acute male subfertility or infertility. KANLA, P.; BURAWAT, J.; ARUN, S.; SAWATPANICH, T.; CHAICHUN, A. & IAMSAARD, S. Acute effects of mimosine purified from Leucaena leucocephala on male reproductive system of adult mice. Int. J. Morphol., 36(2):507-512, 2018.

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“…Mimosine (MiMo) has been proven to have activities against many cancers, cell divisions, cell proliferations and differentiations (Wang et al, 1995;Hughes & Cook;Krude). Indeed, MiMo is a toxic substance in Leucaena leucocephala (LL) (Zayed et al, 2014;Burawat, 2017;Burawat et al, 2016Burawat et al, , 2018. By using Thin Layer Chromatography (TLC) with method validation, Burawat et al (2018) have demonstrated for the first time that MiMo levels in aqueous crude extract of LL shoot tips plus young leaves cultured in Northeastern, Thailand were approximately 17.35 % dry crude extract.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, MiMo is a toxic substance in Leucaena leucocephala (LL) (Zayed et al, 2014;Burawat, 2017;Burawat et al, 2016Burawat et al, , 2018. By using Thin Layer Chromatography (TLC) with method validation, Burawat et al (2018) have demonstrated for the first time that MiMo levels in aqueous crude extract of LL shoot tips plus young leaves cultured in Northeastern, Thailand were approximately 17.35 % dry crude extract. Particularly, both aqueous crude extract of LL shoot tips plus young leaves and purified MiMo have previously reported to have adverse effects on male reproductive mouse organs such as testis, epididymis, and seminal vesicle (Burawat et al, 2018;Kanla et al).…”

Section: Discussionmentioning

confidence: 99%

Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles

et al. 2019

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Acute effect of purified mimosine (MiMo) extracted from Leucaena leucocephala on testicular histopathology has been documented with seminal vesicle (SV) atrophy. Since protein phosphorylation and seminal secretions play important roles in sperm physiology, this study aimed to study the alteration of substances including tyrosine phosphorylated (TyrPho) proteins in seminal vesicle treated with MiMo. Male mice were divided into a control and experimental groups treated with purified MiMo at 3 doses of 15, 30, and 60 mg/KgBW, respectively for 35 consecutive days. The morphology and weights of SV were compared among groups. The levels of magnesium and fructosamine in SV fluid were assayed. The profiles of equally SV total proteins were compared using SDS-PAGE. The expression of seminal TyrPho proteins was detected by western blotting. Recent results showed the decreased weights of SV in MiMo treated mice compared to control. However MiMo in all doses did not affect the levels of magnesium and fructosamine in SV fluid. The SV protein expression of 130 and 55 kDas was obviously decreased in a high dose MiMo. In dose-dependent response, the expressions of 72 and 55 kDas TyrPho proteins of SV were increased. In conclusion, MiMo could affect SV morphological size and protein secretions especially TyrPho proteins.

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Effects of Leucaena leucocephala (Lamk.) Shoot Tips Plus Young Leaf Extract Containing Mimosine on Reproductive System of Male Rats

Cited by 13 publications

References 28 publications

Valproic acid changes the expression of tyrosine‐phosphorylated proteins in rat seminal vesicle

Valproic acid changes the expression of tyrosine‐phosphorylated proteins in rat seminal vesicle

Acute Effects of Mimosine Purified from Leucaena leucocephala on Male Reproductive System of Adult Mice

Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles

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