Effects of Levofloxacin and Doxycycline on Interleukin-6 Production of &lt;i&gt;Chlamydia trachomatis&lt;/i&gt;-Infected Human Synovial Fibroblasts

Ikeda-Dantsuji, Yurika; Konomi, Ichiro; Nagayama, Ariaki

doi:10.1159/000107457

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…With regard to IL-6 expression, we found that both low and high doses significantly affected the increase induced by LPS in doxycycline pretreatment, whereas significant differences were observed only for 0.3 and 0.6 mg/ μ L concentrations in the posttreatment setting. Our data agree with Ikeda-Dantsuji et al who reported that the production of IL-6 was suppressed by the minimum inhibitory concentrations of doxycycline in trachomatis-infected human fibroblast-like synovial cells (HFLS) or HeLa 229 cells immediately or early after infection [ 35 ]. IL-6 is a multifunctional cytokine, produced by monocytes and keratinocytes upon inflammatory stimulation, such as exposure to UV radiation and bacterial endotoxin [ 36 ], whose main functions include activation of lymphocytes and induction of an acute phase response [ 37 ].…”

Section: Discussionsupporting

confidence: 92%

Anti‐Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study

Caprio

Lembo

Costanzo

et al. 2015

Mediators of Inflammation

112

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Doxycycline is used to treat infective diseases because of its broadspectrum efficacy. High dose administration (100 or 200 mg/day) is often responsible for development of bacterial resistances and endogenous flora alterations, whereas low doses (20–40 mg/day) do not alter bacteria susceptibility to antibiotics and exert anti-inflammatory activities. In this study, we wanted to assess the efficacy of both low and high doxycycline doses in modulating IL-8, TNF-α, and IL-6 gene expression in HaCaT cells stimulated with LPS. Three experimental settings were used, differing in the timing of doxycycline treatment in respect to the insult induced by LPS: pretreatment, concomitant, and posttreatment. Low doses were more effective than high doses in modulating gene expression of LPS-induced proinflammatory cytokines (IL-8, TNF-α, and IL-6), when added before (pretreatment) or after (posttreatment) LPS stimulation. This effect was not appreciated when LPS and doxycycline were simultaneously added to cell cultures: in this case high doses were more effective. In conclusion, our in vitro study suggests that low doxycycline doses could be safely used in chronic or acute skin diseases in which the inflammatory process, either constantly in progress or periodically recurring, has to be prevented or controlled.

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Section: Discussionsupporting

confidence: 92%

Anti‐Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study

Caprio

Lembo

Costanzo

et al. 2015

Mediators of Inflammation

112

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“…It should be taken into account that in ammatory state indicated through IL-6-positive cells should be considered higher in 'ON' subjects, especially in the D1R 'ON' group. This estimation is based on the fact, that these subjects were treated with DOX, an antibiotic, that decreases in ammatory processes and thereby IL-6 production and signaling (Di Caprio et al 2015;Ikeda-Dantsuji et al 2007;Kucuk et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Prefrontal dopamine D1 receptor manipulation influences anxiety behavior and induces neuroinflammation within the hippocampus

Beyer¹,

Mattukat²,

Freund³

2020

Preprint

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Background - Prefrontal dopamine D1 receptor (D1R) mediates behavior related to anxiety, reward and memory, and is involved in inflammatory processes, all of which are affected in bipolar disorder. Patients with bipolar disorder show alternations in the dopamingergic and immune system. Interleukin-6 (IL-6), a pro-inflammatory cytokine, is increased in plasma samples, imaging studies and post mortem tissue. Manipulation of the D1R in the medial prefrontal cortex (mPFC) e.g. results in BD-like behavior. The purpose of the study is the investigation of the influence of D1R over-expression and its termination on the immune system and anxiety behavior in rats.Methods – Expression of the gene for D1R in glutamatergic neurons within the mPFC of male, adult rats was manipulated through an inducible (Tet.On) lentiviral vector. Anxiety behavior was studied in the elevated plus maze and marble burying test in ‘ON’ (D1R over-expression) and ‘OFF’ (termination of D1R over-expression) states. IL-6-positive cells were counted to identify the inflammatory state within several subregions of the hippocampus.Results - D1R ‘OFF’ subjects buried more marbles compared to D1R ‘ON’ and their respective control animals indicating an increased anxiety behavior. D1R ‘OFF’ animals reflected an elevated pro-inflammatory state in the hippocampus, in the CA3 and dentate gyrus especially. Consistently, inflammatory state in the whole hippocampus and anxiety behavior correlated positively, indicating a connection between anxiety and inflammatory state of the hippocampus.Conclusions – Behavioral and molecular findings support the association of D1R’s impact on anxiety and inflammation. In addition, by confirming an involvement of IL-6, the new animal model for bipolar disorder has been further validated.

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“…Levofloxacin, like other quinolones, has an immunomodulatory action on the cytokine production at an early transcriptional step of cytokine gene expression [ 32 , 33 ]. Suppression of IL-6 by quinolones has been proven in vitro [ 34 ]. Fluoroquinolones penetrate efficiently into eyes with disrupted epithelium [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation

Fodor

Petrovski

Pásztor

et al. 2014

Journal of Immunology Research

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Objectives. To study the short-term effect of eye opening and use of topical dexamethasone phosphate 0.1% and levofloxacin 0.5% on the cytokine levels in human tears. Methods. Prospective experimental design was used for tear collection from eyes of 10 healthy controls and 20 patients four days after penetrating keratoplasty (PKP) at awakening and after instilling dexamethasone or levofloxacin. The concentrations of different cytokines were measured by cytometric bead array. Results. At eye opening, IL-6 levels were higher in the PKP group as compared to the controls. Thirty minutes later, the released levels of IL-10, IL-13, IL-17, IFNγ, and CCL5 increased in controls, while CXCL8 decreased in both control and PKP groups. The release of the cytokines remained stable after 30 mins except for IFNγ, which showed a decrease in the controls following levofloxacin instillation. No short-term effects of the topically used dexamethasone and levofloxacin could be detected on the cytokine levels in controls and after PKP. Conclusions. Evidence of changes in the levels and time course of tear cytokines after awakening or eye opening could be established and the short-term confounding effects of dexamethasone and levofloxacin on the levels of released cytokines in human tears could be excluded.

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Effects of Levofloxacin and Doxycycline on Interleukin-6 Production of <i>Chlamydia trachomatis</i>-Infected Human Synovial Fibroblasts

Cited by 6 publications

References 42 publications

Anti‐Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study

Anti‐Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study

Prefrontal dopamine D1 receptor manipulation influences anxiety behavior and induces neuroinflammation within the hippocampus

Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation

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