Effects of Light and Elevated Atmospheric CO<sub>2</sub> on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves

Vu, C. V.; Allen, L. H.; Bowes, George

doi:10.1104/pp.73.3.729

Cited by 145 publications

(95 citation statements)

References 11 publications

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“…The data collected on 10/15/87 is not so easily interpreted within this hypothesis. The light levels at which the RuBP pool size remained constant were in a considerably narrower range, similar to the results of Vu et al (29). Also, levels of RuBP were 5-to 8-fold higher than the Rubisco catalytic site concentration in all species at some point during the day.…”

Section: Rubp Levelssupporting

confidence: 75%

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Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Diurnal Changes in Irradiance

Kobza¹,

Seemann²

1989

Plant Physiol.

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The regulation of ribulose-1,5-bisphosphate (RuBP) carboxylase (Rubisco) activity and metabolite pool sizes in response to natural diumal changes in photon flux density (PFD) was examined in three species (Phaseolus vulgaris, Beta vulgaris, and Spinacia oleracea) known to differ in the mechanisms used for this regulation. Diumal regulation of Rubisco activity in P. vulgaris was primarily the result of metabolism of the naturally occurring tight-binding inhibitor of Rubisco, 2-carboxyarabinitol 1-phosphate (CAIP). In B. vulgaris, the regulation of Rubisco activity was the result of both changes in activation state and CAIP metabolism. In S. oleracea, Rubisco activity was regulated by a combination of changes in activation state and the binding/ release of another tight binding inhibitor, probably RuBP. Despite these different mechanisms for the light regulation of Rubisco activity, the relationship between the in vivo activity of Rubisco and the PFD was the same for all three species. Rates of CAIP metabolism were thus sufficient to allow this mechanism to participate in the diumal regulation of Rubisco activity as PFD changed at its normal rate. Furthermore, under natural conditions this regulatory mechanism was found to be important in controlling Rubisco activity over approximately the same range of PFD as did changes in activation state of the enzyme. Finally, this regulation of Rubisco activity resulted in relatively similar and saturating RuBP pool sizes for photosynthesis at all but the lowest PFD values in all three species.

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Section: Rubp Levelssupporting

confidence: 75%

“…Rather, the incident PFD is constantly changing throughout the day. In studies with soybean (Glycine max), the maximum extractable activity of Rubisco varied as PFD changed during a normal daily light course (27,29). We now know this to be a consequence of the metabolism of CAIP.…”

Section: Rubp Levelsmentioning

confidence: 99%

Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Diurnal Changes in Irradiance

Kobza¹,

Seemann²

1989

Plant Physiol.

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“…No general mechanism is known to affect the day-night regulation of RuBisCO (EC 4.1.1.39), although stromal pH and Mg 2+ concentration do. 2-Carboxyarabinitol 1-phosphate (CA1P) may be synthesized in the night to inhibit the activated form of the enzyme in plants including beans, tobacco, and cucumber [2][3][4][5][6][7][8][9][10][11][12][13]. CAIP that accumulates and is bound to RuBisCO in the night is released from the enzyme by RuBisCO activase and degraded [12,14,15].…”

Section: Introductionmentioning

confidence: 99%

Different location in dark‐adapted leaves of Phaseolus vulgaris of ribulose‐1,5‐bisphosphate carboxylase/oxygenase and 2‐carboxyarabinitol 1‐phosphate

1996

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In situ RuBisCO activity was analyzed in order to determine whether 2-carboxyarabinitol 1-phosphate (CAIP) interacts with the enzyme in dark-adapted leaves of Phaseolus vulgaris. Leaves ground to fine powder in liquid nitrogen were put directly into a reaction mixture containing a saturating concentration of ribulose 1,5-bisphosphate (RuBP) to preserve the activity of RuBisCO which was in the chloroplasts. Some 70% of the total catalytic sites of RuBisCO possessed carboxylase activity in this assay, however, RuBisCO was inhibited in the absence of RuBP. CA1P seemed to be concentrated in the veins. These results indicate that RuBisCO was not complexed with CAIP in leaves.

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“…However, the constant value of internal CO2 concentration throughout the experiment suggested that reduced stomatal conductance could not explain lower photosynthetic rates. Spencer and Bowes (15) and Vu et al (18) indicated that the decline of Rubisco2 activity could explain the acclimation to high CO2 levels. However, the hypothesis most often proposed to account for the negative effect of CO2 enrichment is the buildup of starch and sugar.…”

mentioning

confidence: 99%

“…Many species lose their photosynthetic efficiency when subjected for an extended period to high concentrations of CO2 in the atmosphere (5, 9, 12), and become gradually less efficient in the use ofthe added CO2. In a recent study, Yelle et al (21) (18) indicated that the decline of Rubisco2 activity could explain the acclimation to high CO2 levels. However, the hypothesis most often proposed to account for the negative effect of CO2 enrichment is the buildup of starch and sugar.…”

mentioning

confidence: 99%

Acclimation of Two Tomato Species to High Atmospheric CO₂

Yelle¹,

Beeson²,

Trudel³

et al. 1989

Plant Physiol.

153

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Lycopersicon esculentum Mill. cv Vedettos and Lycopersicon chmielewskii Rick, LA1028, were exposed to two CO2 concentrations (330 or 900 microliters per liter) for 10 weeks. Tomato plants grown at 900 microliters per liter contained more starch and more sugars than the control. However, we found no significant accumulation of starch and sugars in the young leaves of L. esculentum exposed to high CO2. Carbon exchange rates were significantly higher in C02-enriched plants for the first few weeks of treatment but thereafter decreased as tomato plants acclimated to high atmospheric CO2. This indicates that the long-term decline of photosynthetic efficiency of leaf 5 cannot be attributed to an accumulation of sugar and/or starch. The average concentration of starch in leaves 5 and 9 was always higher in L. esculentum than in L. chmielewskii (151.7% higher). A higher proportion of photosynthates was directed into starch for L. esculentum than for L. chmielewskii. However, these characteristics did not improve the long-term photosynthetic efficiency of L. chmielewskii grown at high CO2 when compared with L. esculentum. The chloroplasts of tomato plants exposed to the higher CO2 concentration exhibited a marked accumulation of starch. The results reported here suggest that starch and/or sugar accumulation under high CO2 cannot entirely explain the loss of photosynthetic efficiency of high CO2-grown plants.The enhancement of growth and yields by increasing the level of CO2 in the atmosphere has been reported for many species (6). However, the long-term effects of high CO2 levels on the physiological and biochemical behaviors of tomato plants need to be studied further. Many species lose their photosynthetic efficiency when subjected for an extended period to high concentrations of CO2 in the atmosphere (5, 9, 12), and become gradually less efficient in the use ofthe added CO2. In a recent study, Yelle et al. (21) (18) indicated that the decline of Rubisco2 activity could explain the acclimation to high CO2 levels. However, the hypothesis most often proposed to account for the negative effect of CO2 enrichment is the buildup of starch and sugar. Thomas et al. (16), Madsen (7), and Mauney et aL (8) suggested that the decline of photosynthetic efficiency of plants grown at high CO2 concentrations is caused by an accumulation of starch. reported that an accumulation of starch above a critical level causes a decline of CO2 assimilation, and Nafziger and Koller (10) showed that a high starch level is associated with low carbon exchange rates. The buildup of starch can consequently cause a disruption of the thylakoids structure and disorientation ofgrana (7,13

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Effects of Light and Elevated Atmospheric CO₂ on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves

Cited by 145 publications

References 11 publications

Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Diurnal Changes in Irradiance

Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Diurnal Changes in Irradiance

Different location in dark‐adapted leaves of Phaseolus vulgaris of ribulose‐1,5‐bisphosphate carboxylase/oxygenase and 2‐carboxyarabinitol 1‐phosphate

Acclimation of Two Tomato Species to High Atmospheric CO₂

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Effects of Light and Elevated Atmospheric CO2 on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves

Cited by 145 publications

References 11 publications

Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Diurnal Changes in Irradiance

Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Diurnal Changes in Irradiance

Different location in dark‐adapted leaves of Phaseolus vulgaris of ribulose‐1,5‐bisphosphate carboxylase/oxygenase and 2‐carboxyarabinitol 1‐phosphate

Acclimation of Two Tomato Species to High Atmospheric CO2

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Effects of Light and Elevated Atmospheric CO₂ on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves

Acclimation of Two Tomato Species to High Atmospheric CO₂