Effects of Light Exposure, pH, Osmolarity, and Solvent on the Retinal Pigment Epithelial Toxicity of Vital Dyes

Costa, Elaine Fiod; Barros, Nilana M.T.; Coppini, Larissa Pereira; Neves, Raquel Leão; Carmona, Adriana K.; Penha, Fernando M.; Rodrigues, Eduardo B; Dib, Eduardo; Magalhães, Octaviano; Moraes-Filho, Milton N.; Filho, Acácio Alves Souza Lima; Maia, Maurício; Farah, Michel Eid

doi:10.1016/j.ajo.2012.10.004

Cited by 15 publications

(11 citation statements)

References 34 publications

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“…Similarly Awad et al showed a possible protective effect of PEG on RPE cells when exposed to trypan blue [28]. Importantly we also used a significantly shorter contact time than Tura et al and indeed they found toxicity was strongly related to exposure duration as observed with other dyes [29].…”

Section: Discussionsupporting

confidence: 55%

An evaluation of two heavier-than-water internal limiting membrane-specific dyes during macular hole surgery

Steel

Karimi

White

2015

Graefes Arch Clin Exp Ophthalmol

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Section: Discussionsupporting

confidence: 55%

An evaluation of two heavier-than-water internal limiting membrane-specific dyes during macular hole surgery

Steel

Karimi

White

2015

Graefes Arch Clin Exp Ophthalmol

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“…Another case report associated trypan blue, light toxicity, or both with postoperative RPE atrophy [24]. Recent in vitro studies reported that trypan blue is nontoxic to the RPE [25, 26]. The present study demonstrated postoperative foveal RPE abnormalities that were more common (but not statistically significant) in the MB group.…”

Section: Discussionsupporting

confidence: 46%

Macular Hole Surgery with Internal Limiting Membrane Peeling Facilitated by Membrane-Blue® versus Membrane-Blue-Dual®: A Retrospective Comparative Study

Soiberman

Shai

Loewenstein

et al. 2016

Journal of Ophthalmology

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Background. This study aims to compare the outcome of macular hole (MH) surgery with internal limiting membrane (ILM) peeling facilitated by two different vital dyes. Methods. This was a retrospective chart review. The group designated “group-MB” underwent pars plana vitrectomy with ILM peeling facilitated by Membrane-Blue (MB), whereas in “group-MBD,” the vital dye used was Membrane-Blue-Dual (MBD). Results. Seventy-four eyes comprised the study population: 53 in group-MB and 21 in group-MBD. There was no difference in the rate of macular hole closure in group-MB or group-MBD: 71.2% closed MHs compared to 66.7%, respectively (p = 0.7). Postoperative visual improvement was of a higher magnitude in the MBD group compared to the MB group: −0.34 ± 0.81 logMAR versus 0.01 ± 0.06 logMAR, respectively (p = 0.003). Conclusions. In this study, MBD led to better visual results that may be related to better staining characteristics or lesser toxicity compared to MB.

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“…We also expect that the slower degradation of the sequenced PLGAs will minimize the accumulation of pH-lowering acidic by-products compared to random PLGAs. This behavior could provide a protective effect for tissues such the retina which is known to beparticularly sensitive to nonphysiological pH [30]. The improved maintenance of morphology for the sequenced PLGAs could also offer advantages for certain applications, e.g., the repair of craniofacial bony defects, which require longer term mechanical strength during the delivery of critical osteogenic growth factors [31].…”

Section: Introductionmentioning

confidence: 99%

The impact of monomer sequence and stereochemistry on the swelling and erosion of biodegradable poly(lactic-co-glycolic acid) matrices

et al. 2017

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Monomer sequence is demonstrated to be a primary factor in determining the hydrolytic degradation profile of poly(lactic-co-glycolic acid)s (PLGAs). Although many approaches have been used to tune the degradation of PLGAs, little effort has been expended in exploring the sequence-control strategy exploited by nature in biopolymers. Cylindrical matrices and films prepared from a series of sequenced and random PLGAs were subjected to hydrolysis in a pH 7.4 buffer at 37 °C. Swelling ranged from 107% for the random racemic PLGA with a 50:50 ratio of lactic (L) to glycolic (G) units to 6% for the sequenced alternating copolymer poly LG. Erosion followed an inverse trend with the random 50:50 PLGA showing an erosion half-life of 3-4 weeks while poly LG required ca. >10 weeks. Stereosequence was found to play a large role in determining swelling and erosion; stereopure analogs swelled less and were slower to lose mass. Molecular weight loss followed similar trends and increases in dispersity correlated with the onset of significant swelling. The relative proportion of rapidly cleavable G-G linkages relative to G-L/L-G (moderate) and L-L (slow) correlates strongly with the degree of swelling observed and the rate of erosion. The dramatic sequence-dependent variation in swelling, in the absence of a parallel hydrophilicity trend, suggest that osmotic pressure, driven by the differential accumulation of degradation products, plays an important role.

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Effects of Light Exposure, pH, Osmolarity, and Solvent on the Retinal Pigment Epithelial Toxicity of Vital Dyes

Cited by 15 publications

References 34 publications

An evaluation of two heavier-than-water internal limiting membrane-specific dyes during macular hole surgery

An evaluation of two heavier-than-water internal limiting membrane-specific dyes during macular hole surgery

Macular Hole Surgery with Internal Limiting Membrane Peeling Facilitated by Membrane-Blue® versus Membrane-Blue-Dual®: A Retrospective Comparative Study

The impact of monomer sequence and stereochemistry on the swelling and erosion of biodegradable poly(lactic-co-glycolic acid) matrices

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