Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

Chen, Yeng; Huang, Jui Hsien; Hou, Ting-Wen; Pan, I-Chun

doi:10.1186/s40529-019-0257-y

Cited by 48 publications

(41 citation statements)

References 45 publications

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“…Chen et al (2019) reported that root generation and overall plant development can be dramatically promoted under available light intensity conditions in Haworthia [41]. At the same time, light quality is shown to influence ARs in Coleus [42].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Cheng¹,

Han²,

Minrong³

et al. 2020

BMC Genomics

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Background Lotus is an aquatic horticultural crop that is widely cultivated in most regions of China and is used as an important off-season vegetable. The principal root of lotus is degenerated, and adventitious roots (ARs) are irreplaceable for plant growth. We found that no ARs formed under darkness and that exposure to high-intensity light significantly promoted the development of root primordia. Four differential expression libraries based on three light intensities were constructed to monitor metabolic changes, especially in indole-3-acetic acid (IAA) and sugar metabolism. Results AR formation was significantly affected by light, and high light intensity accelerated AR development. Metabolic changes during AR formation under different light intensities were evaluated using gene expression profiling by high-throughput tag-sequencing. More than 2.2 × 104 genes were obtained in each library; the expression level of most genes was between 0.01 and 100 (FPKF value). Libraries constructed from plants grown under darkness (D/CK), under 5000 lx (E/CK), and under 20,000 lx (F/CK) contained 1739, 1683, and 1462 upregulated genes and 1533, 995, and 834 downregulated genes, respectively, when compared to those in the initial state (CK). Additionally, we found that 1454 and 478 genes had altered expression in a comparison of libraries D/CK and F/CK. Gene transcription between libraries D/F ranged from a 5-fold decrease to a 5-fold increase. Twenty differentially expressed genes (DEGs) were involved in the signal transduction pathway, 28 DEGs were related to the IAA response, and 35 DEGs were involved in sugar metabolism. We observed that the IAA content was enhanced after seed germination, even in darkness; this was responsible for AR formation. We also observed that sucrose could eliminate the negative effect of 150 μMol IAA during AR development. Conclusions AR formation was regulated by IAA, even in the dark, where induction and developmental processes could also be completed. In addition, 36 genes displayed altered expression in carbohydrate metabolism and ucrose metabolism was involved in AR development (expressed stage) according to gene expression and content change characteristics.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Cheng¹,

Han²,

Minrong³

et al. 2020

BMC Genomics

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“…It is an evidence that light intensity and light quality affect adventitious root development and the photosynthate quantity of Hypericum perforatum [41]. report that root generation and development can be dramatically promoted under available light intensity condition in Haworthia [42]. At the same time, light quality is shown to influence ARs in number [43].…”

Section: Discussionmentioning

confidence: 97%

Gene expression profiling reveals light regulation on adventitious root formation in lotus (Nelumbo nucifera Gaertn.)

Cheng¹,

yuyan²,

minrong³

et al. 2020

Preprint

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Background: Lotus is an aquatic horticultural crop, and widely cultivated in most regions of China.Lotus is often used as a kind of an important off-season vegetable with various nutrients. Principle root of lotus is degenerated, and the role of adventitious roots (ARs) is Irreplaceable for plant growth.We found that no ARs could be formed under darkness condition, and high light significantly promote the development of root primordium. Therefore, four libraries with three light intensities were constructed to monitor metabolism changes, especially in IAA and sugar metabolism.Results: We found that ARs formation was significantly affected by light, high light intensity accelerated ARs development. The change of metabolism during ARs formation under different light intensity was evaluated according to gene expression profiling by high-throughput tag-sequencing. It was shown that more than 2.2× 10 4 genes was obtained in each library, and the expression level of most genes were distributed between 1e-01 and 1e+03 (FPKF value). Among these identified genes, 1739, 1683 and 1462 genes were up-regulated, and 1533, 995 and 834 genes were down-regulated in D/CK, E/CK and F/CK libraries respectively. In addition, we also found that 1454 and 478 genes changed expression in D/CK and F/CK libraries when compared D/CK with F/CK libraries. In F/D libraries, the transcriptional level of most differentially expressed genes was between -5~5 fold, and twenty differentially expressed genes were involved in signal transduction pathway. Twenty-eight genes related with IAA response and thirty-five genes involved in sugar metabolism were found to change expression. It was elucidated that IAA content was enhanced after seed germinated, even in darkness condition, which was responsible for ARs formation.Conclusion: The process of ARs formation was very complex, and regulated by multiple factors. The ARs formation was regulated by IAA, even in the dark, induction and developmental process could also be completed. In addition, the genes (36 genes) changed expression level in carbohydrate metabolism showed the third number, so sucrose metabolism was involved in the ARs development (expressed stage) according to genes expression and content change characteristic.

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“…In addition, Wahyuni et al [60] stated that the colour of callus becomes brown because they produced phenolic compounds and the variation in callus morphology is due to the explant variant, basic medium, growth regulators, and the biotic and abiotic supplements in the culture. In tissue culture, plant growth regulators play significant role in controlling plant growth and development [61]. Auxins are the major hormones responsible for stem elongation, vascular tissue differentiation, and cell expansion in plant developmental processes [62].…”

Section: In Vitro Plant Materials Propagationmentioning

confidence: 99%

Natural Surfactant Saponin from Tissue of Litsea glutinosa and Its Alternative Sustainable Production

Wisetkomolmat

Suksathan²,

Puangpradab³

et al. 2020

Plants

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In this research, we assessed the detergency properties along with chemical characteristic of the surfactant extracts from the most frequently cited detergent plants in Northern Thailand, namely, Sapindus rarak, Acacia concinna, and Litsea glutinosa. Moreover, as to provide the sustainable option for production of such valuable ingredients, plant tissue culture (PTC) as alternative method for industrial metabolite cultivation was also proposed herein. The results illustrated that detergent plant extracts showed moderate in foaming and detergency abilities compared with those of synthetic surfactant. The phytochemical analysis illustrated the positive detection of saponins in L. glutinosa plant extracts. The highest callus formation was found in L. glutinosa explant cultured with MS medium supplemented with 2.0 mg/L Indole-3-acetic acid (IAA). The callus extract was chemical elucidated using chromatography, which illustrated the presence of saponin similar to those from the crude leaf and Quillaja saponin extracts. Compact mass spectrometry confirmed that the surfactant was of the steroidal diagnostic type.

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Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia

Cited by 48 publications

References 45 publications

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Gene expression profiling reveals light regulation on adventitious root formation in lotus (Nelumbo nucifera Gaertn.)

Natural Surfactant Saponin from Tissue of Litsea glutinosa and Its Alternative Sustainable Production

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