Effects of Light Quality on Vegetative Growth and Flower Initiation in Phalaenopsis

Abstract

The effects of LEDs (Light-Emitting Diodes) emitting different colours namely red, blue, red and blue, and white lights on vegetative growth and fl ower initiation of Phalaenopsis have been evaluated. Phalaenopsis"otohine/taisuco fi re bird" seedlings in vitro were subjected to different light qualities for either 2 or 4 weeks, and then each seedling was planted in a plastic pot containing sphagnum and grown in the growth chamber under similar light quality for 3 months. For fl ower induction, mature Phalaenopsis plants having 4 -6 leaves were grown for 3 months in the growth chamber under different light qualities. The leaf span, chlorophyll, gibberellin and cytokinin content were determined. In addition, the expressions of FT-like gene in the leaf, axillary bud, fl ower bud and stalk were examined.Vegetative growth was enhanced under blue, red-blue or white LEDs compared to that of the control. Gibberellin and cytokinin content increased in the seedlings subjected to white LEDs. Based on the average of leaf span increment it was suggested that the growth of Phalaenopsis seedlings can be promoted by giving either blue, red-blue or white LEDs. From the second experiment, it was found that fl ower induction in Phalaenopsis can be obtained in plants that had just fi nished fl owering without the application of LEDs. The expression of FT-like gene in the leaf as well as fl ower bud and stalk suggests that this gene is involved in fl ower regulation of Phalaenopsis.

Pengenalan Dan Pelatihan Budidaya Anggrek Dalam Rangka Pemanfaatan Lahan Wana Desa

“…Morfologi anggrek dapat berbagai macam, misalnya berbatang ramping atau berdaging (Purwanto, 2016). Anggrek memerlukan cahaya 75-85% dengan lama penyinaran 8 jam/hari untuk pertumbuhan vegetative dan generatifnya (Dewi et al, 2015). Meskipun demikian, anggrek Phalaenopsis mampu tumbuh baik pada intensitas cahaya 30 -60% dengan kelembaban 60 -80% (Handini et al, 2016;Pangestu et al, 2015).…”

“…Meskipun demikian, anggrek Phalaenopsis mampu tumbuh baik pada intensitas cahaya 30 -60% dengan kelembaban 60 -80% (Handini et al, 2016;Pangestu et al, 2015). Sinar matahari merupakan factor penting dalam pertumbuhan dan perkembangan tanaman (Dewi et al, 2015;Putra et al, 2016). Di sisi lain, anggrek tidak tahan pada kondisi air yang berlebihan (Andalasari et al, 2014).…”