Effects of light, temperature, pH, and inhibitors on the ATP level of the blue-green alga Anacystic nidulans

Bornefeld, T.; Simonis, W.

doi:10.1007/bf00388613

Cited by 39 publications

(16 citation statements)

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“…Marked increase of ATP content in leaves of cold-grown plants observed in the light may be due to an excess of photophosphorylation over carbon assimilation at low temperature (2,9,12). However, we observed that ATP and TAP content and energy charge value in the cold-treated leaves were even higher in darkness than in the light.…”

Section: Discussionmentioning

confidence: 99%

Adenine Nucleotide Changes during Cold Acclimation of Winter Rape Plants

Sobczyk¹,

Kacperska‐Palacz²

1978

Plant Physiol.

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immediately frozen in liquid N2. All of the harvests were performed in the 2nd or in the 16th hr of the light period or after different periods of darkness (2 and 5 min and 10 hr). Frozen tissues were powdered, lyophilized using Edwards High Vacuum LTD, model EF2 and stored over CaCl2 at -27 C for 1 or 2 weeks.Simultaneously, the fresh and dry weights oftissues, comparable to those taken for nucleotides analysis, were determined.Extraction and Determination of Nucleotides. Adenine nucleotides were extracted by putting 25 mg of dried tissue into 10 ml of boiling deionized H20 for 3 min. The extract was then rapidly cooled in an ice bath and centrifuged at 0 C to remove insoluble residue. The supernatant fraction was used directly for an assay. In order to determine the ATP, ADP, and AMP contents, 0.2 ml of the extract was incubated in HEPES buffer with P-enolpyruvate, pyruvate kinase, and adenylate kinase according to Pradet (15). After incubation samples were immediately assayed by the luciferine-luciferase method, according to Saint John (16). A Beckman liquid scintillation system (model LS-100) was used to measure light produced in the reaction.Enzymes and Chemicals. All enzymes and chemicals were products of Sigma Chemical Company. RESULTSFrost tolerance of leaves increased slightly during plant growth at 5 C for 8 days (Fig. 1) and the marked increase of Tk2 to -14 C was noted after lowering the temperature to 0 C. However, the cold treatment did not increase the frost tolerance of roots (Fig. 1, upper curves): their Tk. varied from -4 C to -5.5 C and did not change during the whole hardening period.The content of ATP in the control, non-cold-treated tissue did not change significantly in the course of experiment (Fig. 2). Fluctuations observed in roots were probably due to the changeable proportion of young and older roots in the sample. Some ATP increase noted in roots at the end of experiment was probably due to the new root formation which usually occurs when plants are cultured in a water medium.When plants were subjected to cold treatment significant changes in ATP content occurred in the tissues studied (Fig. 2). In roots, ATP content was maintained at the level of the control during the first 4 days of cold treatment and then, after 8 and 14 days it decreased to about 50% of the initial value.In leaves, a 2-fold increase of ATP content was noted during the first 4 days of the cold treatment which persisted as the cold treatment continued. Decreasing the temperature from 5 to 0 C had no further effect on ATP content. Since the cold treatment brought about marked changes of both frost tolerance and ATP content in leaves only, further experiments were performed on that tissue.In order to check whether the higher ATP content in leaves of frost-tolerant plants was due only to photosynthetic phosphoryl-

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Section: Discussionmentioning

confidence: 99%

Adenine Nucleotide Changes during Cold Acclimation of Winter Rape Plants

Sobczyk¹,

Kacperska‐Palacz²

1978

Plant Physiol.

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“…Earlier investigations indicate that metabolic processes that support cell growth cycles in light are curtailed in the dark [16,[40][41][42][43]. The experimental data suggest that chemical energy was neither produced nor consumed in the dark in sufficient levels to support cell growth cycle 666 Y. Asato Cell cycle of unicellular cyanobacteria in the dark.…”

Section: Effects Of Light and Dark Shifts On Cellular Metabolism Genmentioning

confidence: 92%

Toward an understanding of cell growth and the cell division cycle of unicellular photoautotrophic cyanobacteria

Asato¹

2003

Cellular and Molecular Life Sciences (CMLS)

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The cell division cycle of Synechococcus sp. strain PCC 6301 in light is characterized by the sequential and orderly appearance of macromolecular synthesis periods. In the dark, macromolecular synthesis and cell division are severely curtailed. When dark-incubated cultures are reexposed to light, a new cell cycle is initiated. The pattern of the cell events displayed by Synechococcus in light and the absence of sustained growth in dark incubation conditions suggests that light-activated regulatory molecules control macromolecular synthesis and the cell division cycle. For example, ribosomal RNA synthesis is stimulated by a light-activated DNA binding factor in light but not in the dark. Light/dark conditions induce cell synchrony in Prochlorococcus. Distinct G1, S and G2 phases characterize cell cycles of marine Synechococcus and Prochlorococcus. Cell division in Synechococcus elongatus PCC 7942 and marine Synechococcus is controlled by circadian oscillators.

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“…(Bornefeld and Simonsis, 1974). The treatment of cells with DCMU resulted in inhibition of CA activity, and the residual activity could be attributed to cyclic photophosphorylation (Codd and Cossar, 1978).…”

Section: Discussionmentioning

confidence: 97%