Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

Abstract

SUMMARY:The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental step in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and Cshowed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit® machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.

Mesenchymal Stem Cell Signaling Pathway and Interaction Factors

“…Treatments on the cellular level is mostly done by using autologous bone marrow MSC and fat tissue which reserves a large number of MSC when compared to bone marrow (3). A large part of the stem cells in the adipose tissue are located in the perivascular region (4), and they have a unique capacity for proliferation and transdifferentiation (5). MSCs have the capacity to differentiate into adipocyte, osteocyte, chondrocyte, myocyte and neuronal cell lines.…”