Effects of lipopolysaccharide administration and maternal deprivation on anxiety and depressive symptoms in male and female Wistar rats: Neurobehavioral and biochemical assessments

Benmhammed, Hajar; Hayek, Samer El; Nassiri, Abdeljabbar; Bousalham, Rim; Mesfioui, Abdelhalem; Ouichou, Ali; Hessni, Aboubaker El

doi:10.1016/j.bbr.2019.01.005

Cited by 17 publications

(15 citation statements)

References 117 publications

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“…Finally, the brain regions of each hemisphere (striatum, hippocampus and prefrontal cortex) were quickly dissected on ice, frozen by liquid nitrogen, and stored at −80°C until the analysis of TNFalpha content. The Dounce homogenizer was used to homogenize tissues in an ice-cold lysis buffer (RIPA lysate solution + 1 mM PMSF), and the homogenates were centrifuged for 15 minutes (14 000 g), and then stored at −80°C for the subsequent ELISA assays as previously described by Benmhammed et al (35).…”

Section: Preparation Of Homogenatesmentioning

confidence: 99%

Phenolic fraction concentrates supplementation ameliorates learning and memory impairments in chronically stressed streptozotocin-diabetic rats by reducing brain tumor necrosis factor-α

Bikri

Fath

Aboubi

et al. 2022

J Herbmed Pharmacol

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Introduction: The present work aims to assess if insulin combined with phenolic fraction concentrates (PFCs) prevents diabetes-related cognitive impairments by controlling neuroinflammation in streptozotocin-induced diabetic rats exposed to chronic mild stress (CMS). Methods: Directly after confirming the hyperglycemia, diabetic animals were treated with insulin combined with PFC and were exposed to 2 stressors/day for 12 weeks. Then, four cognitive tests were carried out to assess learning and memory performances. Finally, the rats were anesthetized, blood samples were collected for corticosterone and Tumor necrosis factor alpha (TNF-α) analysis, and the brain regions viz. striatum, hippocampus, and prefrontal-cortex of each hemisphere were dissected out for TNF-α analysis. Results: Both diabetes and stress could induce learning and memory impairments, which were more prominent in stressed diabetic animals, and significantly reversed by insulin treatment supplemented with PFC compared to the insulin monotherapy. Moreover, diabetic rats exposed to CMS displayed disturbances in glucose homeostasis as well as corticosterone secretion. These dysfunctions were linked to the significant increase of TNF-α in the blood as well as in the prefrontal cortex, hippocampus, and striatum. Insulin significantly ameliorated this inflammatory abnormality, while the supplemented treatment showed a significant effect, by stabilizing TNF-α to its normal levels in the hippocampus and in the blood when compared to insulin monotherapy. Conclusion: Insulin supplemented with PFC has a favorable effect over insulin alone on inflammatory aberrations linked with type 1 diabetes and stress in animals, confirming the preference of the combined treatment over insulin for the management of cognitive impairment in stressed diabetic subjects.

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Section: Preparation Of Homogenatesmentioning

confidence: 99%

Phenolic fraction concentrates supplementation ameliorates learning and memory impairments in chronically stressed streptozotocin-diabetic rats by reducing brain tumor necrosis factor-α

Bikri

Fath

Aboubi

et al. 2022

J Herbmed Pharmacol

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“…To investigate the anxiety-like behavior in rats, the open field test (OFT) was performed as described previously (23). Briefly, during the test day, each rat was placed on the central platform and allowed to explore the apparatus freely for 10 minutes.…”

Section: Open Field Testmentioning

confidence: 99%

“…Animals are individually placed on the dark side facing the door. We measured the time spent in the white compartment for 6 minutes (23).…”

Section: Light-dark Box Testmentioning

confidence: 99%

“…After sacrificing the rat, the prefrontal cortex, striatum, and hippocampus of each hemisphere were collected and separately dissected. The Dounce homogenizer was used to homogenize tissues in an ice-cold lysis buffer (RIPA lysate solution + 1 mM PMSF), and the homogenates were centrifuged for 15 minutes (14 000 g), then stored at −80°C (23). The pancreas and adrenal gland tissues were prepared following the protocol described by Erejuwa et al (25).…”

Section: Preparation Of Homogenatesmentioning

confidence: 99%

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Insulin supplemented with phenolic fraction concentrates displays anxiolytic and antidepressant-like properties with reductions of oxidative brain damage in chronically stressed diabetic rats

Bikri

Talhaoui

Fath

et al. 2022

J Herbmed Pharmacol

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Introduction: The current study aimed to investigate if insulin supplemented with phenolic fraction concentrates (PFC) improves chronic hyperglycemia-related behavioral changes by mitigating oxidative stress in diabetic rats exposed to chronic mild stress (CMS). Methods: Experimental type 1 diabetes mellitus (T1DM) was established by a single intraperitoneal injection of streptozotocin (STZ, 65 mg/kg). After diabetes confirmation, rats were treated with insulin supplemented with PFC and exposed to two unpredictable mild stressors per day for 12 weeks. Body weight changes, fasting blood glucose (FBG), and corticosterone levels were evaluated. The behavioral tests were performed to evaluate anhedonia, anxiety, and depressive-like behaviors. Twenty-four hours after behavioral tests, all rats were anesthetized, and the blood was collected for the analysis of lipid, hepatic, and renal parameters. Finally, the brain areas (striatum, hippocampus, and prefrontal cortex), pancreas, and adrenal glands were dissected for the analysis of oxidative stress markers. Results: The results of this study revealed that treatment with insulin supplemented with PFC for 12 weeks significantly enhanced antioxidant defenses (catalase [CAT] and superoxide dismutase [SOD]) and reduced oxidative stress damage (nitric oxide and malondialdehyde [MDA]), especially in brain regions (prefrontal cortex, hippocampus, and striatum) in stressed diabetic rats (P < 0.001). This combination also ameliorated the corticosterone level (P < 0.001) as well as glucose homeostasis (P < 0.001) and lipid parameters (P < 0.001), which are markedly altered in T1D associated with stress. Conclusion: The associated treatment possesses important anxiolytic and antidepressant-like effects in this rat model, which might be mainly mediated by its capacity to protect brain cells against reactive oxygen species (ROS) triggered by T1DM and/or chronic stress.

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“…Studies have demonstrated that microglia have functional receptors to sense and respond to stress hormones and neuroendocrine signals in early development [ 55 , 74 ], and psychological stressors during development can have long lasting effects on the brain and behavior [ 5 , 75 , 76 ]. To model human developmental stress in rodents, commonly used stressors include daily restraint and light stress to pregnant dams, maternal sleep deprivation, or maternal separation (MS) of the neonatal pups.…”

Section: Developmental Stressors’ Impact On Brain Microgliamentioning

confidence: 99%

Developmental Stressors Induce Innate Immune Memory in Microglia and Contribute to Disease Risk

Carloni

Ramos

Hayes

2021

IJMS

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Many types of stressors have an impact on brain development, function, and disease susceptibility including immune stressors, psychosocial stressors, and exposure to drugs of abuse. We propose that these diverse developmental stressors may utilize a common mechanism that underlies impaired cognitive function and neurodevelopmental disorders such as schizophrenia, autism, and mood disorders that can develop in later life as a result of developmental stressors. While these stressors are directed at critical developmental windows, their impacts are long-lasting. Immune activation is a shared pathophysiology across several different developmental stressors and may thus be a targetable treatment to mitigate the later behavioral deficits. In this review, we explore different types of prenatal and perinatal stressors and their contribution to disease risk and underlying molecular mechanisms. We highlight the impact of developmental stressors on microglia biology because of their early infiltration into the brain, their critical role in brain development and function, and their long-lived status in the brain throughout life. Furthermore, we introduce innate immune memory as a potential underlying mechanism for developmental stressors’ impact on disease. Finally, we highlight the molecular and epigenetic reprogramming that is known to underlie innate immune memory and explain how similar molecular mechanisms may be at work for cells to retain a long-term perturbation after exposure to developmental stressors.

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Effects of lipopolysaccharide administration and maternal deprivation on anxiety and depressive symptoms in male and female Wistar rats: Neurobehavioral and biochemical assessments

Cited by 17 publications

References 117 publications

Phenolic fraction concentrates supplementation ameliorates learning and memory impairments in chronically stressed streptozotocin-diabetic rats by reducing brain tumor necrosis factor-α

Phenolic fraction concentrates supplementation ameliorates learning and memory impairments in chronically stressed streptozotocin-diabetic rats by reducing brain tumor necrosis factor-α

Insulin supplemented with phenolic fraction concentrates displays anxiolytic and antidepressant-like properties with reductions of oxidative brain damage in chronically stressed diabetic rats

Developmental Stressors Induce Innate Immune Memory in Microglia and Contribute to Disease Risk

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