Effects of Local Administration of Vascular Endothelial Growth Factor on Mechanical Characteristics of the Semitendinosus Tendon Graft after Anterior Cruciate Ligament Reconstruction in Sheep

Yoshikawa, Toshikazu; Tohyama, Harukazu; Katsura, Taro; Kondo, Eiji; Kotani, Yoshihisa; Matsumoto, Hideo; Toyama, Yoshiaki; Yasuda, Kazunori

doi:10.1177/0363546506294469

Cited by 157 publications

(165 citation statements)

References 23 publications

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“…11,41 In the present study, biomechanical testing showed significant improvements in the functional healing of the ligaments in the MDSC-VEGF and the MDSC groups; however, there was no significant difference observed between these 2 groups. Some studies have reported that VEGF was produced at its highest levels only after the inflammatory phase in the natural healing process of the injured ligament and tendon and that VEGF and blood vessel formation crested between 5 and 9 days post-injury.…”

Section: Discussioncontrasting

confidence: 53%

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Role of angiogenesis after muscle derived stem cell transplantation in injured medial collateral ligament

Nishimori

Matsumoto

Ota

et al. 2011

Journal Orthopaedic Research

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We performed this study to investigate the therapeutic role of vascular endothelial growth factor (VEGF) in medial collateral ligament (MCL) healing. Murine muscle derived stem cells (MDSCs) obtained via the preplate technique were retrovirally transduced to express: (1) VEGF and nLacZ (MDSC-VEGF), (2) soluble fms-like tyrosine kinase-1 (sFLT1, a VEGF-specific antagonist) and nLacZ (MDSC-sFLT1), and (3) nLacZ (MDSC-nLacZ). After transecting the MCL of immunodeficient rats, 5 Â 10 5 cells of each of the transduction groups list above were transplanted into the MCL injury site. A control group was injected with phosphate-buffered saline (PBS) only. Immunohistochemical staining demonstrated that there were more Isolectin B4 and b-galactosidase double positive cells in the rats transplanted with MDSC-VEGF transduced cells than the other groups at week 1. Capillary density was significantly higher in the MDSC-VEGF group than the other groups at week 2; however, there were no significant differences in the biomechanical assessment between the MDSC-VEGF and MDSC-nLacZ groups. On the other hand, the MDSC-sFLT1 group revealed a lower capillary density than the other two groups and the functional ligament healing of the MDSC-sFLT1 group was significantly decreased compared to the other groups when assessed biomechanically. The findings of the present study suggest that angiogenesis plays a critical role in the healing process of injured MCL. ß

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Section: Discussioncontrasting

confidence: 53%

“…[9][10][11] Among the variety of growth factors regulating angiogenesis, 12 the most potent known is the vascular endothelial growth factor (VEGF). 13 Recent studies have shown that VEGF plays an important role in tissue healing through angiogenesis induction.…”

mentioning

confidence: 99%

Role of angiogenesis after muscle derived stem cell transplantation in injured medial collateral ligament

Nishimori

Matsumoto

Ota

et al. 2011

Journal Orthopaedic Research

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“…Kobayashi et al 26 indicated that enhanced neovascularization and formation of granulation tissue induced the healing of a partially injured ACL with growth factor. A sheep study by Yoshikawa et al 27 using a VEGF solution demonstrated that newly formed vessels and infiltrative fibroblasts were more abundant in tendons that had been soaked in VEGF solution prior to being grafted into a ruptured ACL than in tendons that had been soaked in PBS. Unfortunately, growth factor is very expensive.…”

Section: Angiogenesis After Implantation In Vivomentioning

confidence: 99%

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Seo

Yoon

Song

et al. 2009

Journal Orthopaedic Research

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The purpose of this study was to evaluate the biocompatibility of silk and collagen-hyaluronan (HA) in vitro by assessing anterior cruciate ligament (ACL) cell and T-lymphocyte cultures on scaffolds. The use of composite scaffolds as artificial ligaments in ACL reconstruction and their effects on angiogenesis were evaluated in vivo. The silk scaffold was knitted by hand and dry coated with collagen-HA, whereas the composite silk scaffold was made by covering a silk scaffold with a lyophilized collagen-HA substrate. The initial attachment and proliferation of human ACL cells on the composite silk scaffold was superior to the attachment and proliferation observed on the silk scaffold. The immune response was higher in both scaffolds after 72 h ( p < 0.05) compared with the control culture condition without scaffolding, as assessed by T-lymphocyte cultures in vitro. There was no significant difference in the immune response in vitro between the silk and composite silk scaffolds. Silk and composite silk scaffolds were implanted as artificial ligaments in ACLs removed from the knees of dogs, and they were harvested 6 weeks after implantation. On gross examination, the onset of an inflammatory tissue reaction, such as synovitis, was seen in both the silk scaffold and the composite silk scaffold groups. An histological evaluation of the artificial ligament implants revealed the presence of monocytes in the silk composite scaffold and the absence of giant cells in all cases. MT staining in the composite silk scaffold-grafted group showed granulation tissue consisting of fibroblasts, lymphocytes, monocytes, and collagen fibers. In addition, CD31 staining revealed the formation of new blood vessels. On the other hand, no reparative tissues, such as blood vessels, collagen, and cells, were observed in the silk scaffold-grafted group. These results suggest that the lyophilized collagen-HA substrate is biocompatible in vitro and enhances new blood vessel and cell migration in vivo. ß

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“…Therefore, we conducted a following large animal model study to clarify if recombinant VEGF application affects the mechanical properties of the grafted tendon after ACL reconstruction before its clinical application of recombinant VEGF therapy to ACL reconstruction. In this experiment, we used mature female Suffolk sheep (Yoshikawa, 2006b) . We harvested the semitendinosus tendon from the right leg and then soaked the tendon in recombinant human VEGF with 10-ml phosphate buffered saline (PBS) for 15 minutes and then perfrmed ACL reconstruction using this semitendinosus tendon in the same leg (Fig.…”

Section: Growth Factor Application For Enhancement Of Angiogenesismentioning

confidence: 99%

“…Ju (2006)). Yoshikawa (2006b)). Group I: the femur-graft-tibia complex 12 weeks after ACL reconstruction with semitendinosus tendon graft soaked in phosphate buffered saline, Group II: the femur-graft-tibia complex 12 weeks after ACL reconstruction with semitendinosus tendon graft soaked in VEGF solution, Normal: normal the femur-graft-tibia complex with no treatment.…”

Section: Growth Factor Application For Enhancement Of Angiogenesismentioning

confidence: 99%

Application of Growth Factors for Enhancement of Mechanical Strength of Grafted Tendon Following Anterior Cruciate Ligament Reconstruction

Tohyama¹,

Yasuda²

2011

Biomechanics in Applications

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Effects of Local Administration of Vascular Endothelial Growth Factor on Mechanical Characteristics of the Semitendinosus Tendon Graft after Anterior Cruciate Ligament Reconstruction in Sheep

Cited by 157 publications

References 23 publications

Role of angiogenesis after muscle derived stem cell transplantation in injured medial collateral ligament

Role of angiogenesis after muscle derived stem cell transplantation in injured medial collateral ligament

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Application of Growth Factors for Enhancement of Mechanical Strength of Grafted Tendon Following Anterior Cruciate Ligament Reconstruction

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