Effects of Local Nasal Immunotherapy with FIP-fve Peptide and Denatured Tyrophagus putrescentiae for Storage Mite-Induced Airway Inflammation

Yen, Ching‐Yu; Yu, Ching-Hsiang; Tsai, Jaw‐Ji; Tseng, Hsiang-Kuang; Liao, En-Chih

doi:10.1007/s00005-022-00645-w

Cited by 7 publications

(9 citation statements)

References 74 publications

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“…The experimental procedures and operation methods of the asthmatic animal model mainly referred to our previous study [ 15 ]. Mice were sensitized three times by intraperitoneal (IP) injection with 10 µg Der p 2 emulsified in 4 mg aluminum hydroxide on day 1, day 7, and day 14 (Sigma-Aldrich, St. Louis, MO, USA) (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The HDM-induced asthma animal model has been reported to be useful for the evaluation of the effectiveness of immunotherapy [ 14 ]. Intraperitoneal sensitization followed by intratracheal challenge with mite extract can induce airway inflammation, tracheal rupture, and tracheal thickening in the asthmatic mouse model [ 15 ]; therefore, this asthmatic model is suitable to evaluate the therapeutic effectiveness of candidate medicine like Traditional Chinese Medicine (TCM) on mite-induced allergic responses [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory effects of modified Liu-Wei-Di-Huang-Wan Traditional Chinese medicine on allergic asthmatic mice

Tsai

Yen

Hsu

et al. 2023

Allergy Asthma Clin Immunol

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Background Allergic asthma occurs worldwide and is particularly prevalent in westernized countries characterized by chronic airway inflammation resulting in airway hyperresponsiveness. The house dust mites (HDM) including Dermatophagoides pteronyssinus are major sources of sensitization and triggering allergic symptoms in asthmatic patients. The Der p 2 is a major allergen and the predominant source of causative respiratory disorders which induce airway inflammation and bronchial constriction in mite-allergic patients. Few studies evaluate the ameliorating effects of modified Liu-Wei-Di-Huang-Wan (modified LWDHW) on allergic asthma. Methods This study aimed to investigate the immunological mechanisms of modified LWDHW on the reductions of airway inflammation, signal transduction, inflammatory cytokine production, Th2 cell proliferation, and bronchial obstruction in Der p 2-induced asthmatic mice. Results At least ten active ingredients were contained in the formula of modified LWDHW- 1217A and 1217B. Results showed that the immunoglobulin generations (Der p 2 specific- IgE and IgG1), inflammatory cytokine productions (IL-5 and IL-13) in the Sera and BALF could be down-regulated, and the Th1-cytokine productions (IL-12 and IFN-γ) be increased after immunotherapy with modified LWDHW of 1217A or 1217B. The inflammatory cell infiltrations (macrophages, eosinophils, and neutrophils) in the airway and the expressions of TH2-related genes (IL-4, IL-5, and IL-13), TH2-related transcription factor (GATA-3), and neutrophil chemotactic chemokine (IL-8) in the lung tissue of asthmatic mice were significantly decreased after the immunotherapy. The Th1/Th2 polarization had been identified that the IL-4+/CD4+ T cells were downregulated and IFN-γ+/CD4+ T cells were increased. The airway hyperresponsiveness to methacholine inhalation of Penh values was significantly decreased in the treated groups. There were significant improvements in the bronchus histopathology after immunotherapy with 1217A or 1217B which were evaluated by tracheal thickness, inflammatory cell count, and tracheal rupture of mouse lung. Conclusion It revealed that 1217A or 1217B could regulate the immune responses and improve pulmonary function. Data suggests that modified LWDHW of 1217A or 1217B have the potential for use as a therapeutic intervention for the treatment of mite allergen Der p 2-induced allergic asthma.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of modified Liu-Wei-Di-Huang-Wan Traditional Chinese medicine on allergic asthmatic mice

Tsai

Yen

Hsu

et al. 2023

Allergy Asthma Clin Immunol

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“…Moreover, new routes of administration are currently being researched, which could perform a new impact on meeting patients’ needs and prerogatives, improving the adherence to therapy; however, they still require more detailed studies. Intralimphatic immunotherapy (ILIT), for example, may result in a reduction in the frequency of administration; epicutaneous immunotherapy (EPIT) may open new scenarios for the treatment of food allergy especially in children [ 41 ], and the anti-inflammatory effect of local nasal immunotherapy (LNIT) with FIP-fve peptide and denatured mite Tyrophagus putrescentiae may be exploited for the treatment of airway allergic diseases [ 42 , 43 ] ( Figure 1 ).…”

Section: Future Landscape Of Personalized Aitmentioning

confidence: 99%

The Present and Future of Allergen Immunotherapy in Personalized Medicine

Ridolo

Incorvaia

Heffler

et al. 2022

JPM

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Allergic diseases are particularly suitable for personalized medicine, because they meet the needs for therapeutic success, which include a known molecular mechanism of the disease, a diagnostic tool for that disease and a treatment that blocks this mechanism. A range of tools is available for personalized allergy diagnosis, including molecular diagnostics, treatable traits and omics (i.e., proteomics, epigenomics, metabolomics, transcriptomics and breathomics), to predict patient response to therapies, detect biomarkers and mediators and assess disease control status. Such tools enhance allergen immunotherapy. Higher diagnostic accuracy results in a significant increase (based on a greater performance achieved with personalized treatment) in efficacy, further increasing the known and unique characteristics of a treatment designed to work on allergy causes.

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“…The experimental procedures and operation methods of the asthmatic animal model mainly referred to our previous study 15 . Mice were sensitized three times by intraperitoneal (IP) injection with 10 µg Der p 2 emulsi ed in 4 mg aluminum hydroxide on day 1, day 7, and day 14 (Sigma-Aldrich, St. Louis, MO, USA) (Fig.…”

Section: Experimental Schedulementioning

confidence: 99%

“…The HDMinduced asthma animal model has been reported to be useful for the evaluation of the effectiveness of immunotherapy 14 . Intraperitoneal sensitization followed by intratracheal challenge with mite extract can induce airway in ammation, tracheal rupture, and tracheal thickening in the asthmatic mouse model 15 ; therefore, this asthmatic model is suitable to evaluate the therapeutic effectiveness of candidate medicine like Traditional Chinese Medicine (TCM) on mite-induced allergic responses 15 .…”

Section: Introductionmentioning

confidence: 99%

Effects of traditional Chinese medicine on allergic asthmatic mice: using a combined formula

Tsai

Yen

Hsu

et al. 2022

Preprint

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Background Allergic asthma occurs worldwide and is particularly prevalent in westernized countries characterized by chronic airway inflammation resulting in airway hyperresponsiveness. The house dust mites (HDM) including Dermatophagoides pteronyssinus are major sources of sensitization and triggering allergic symptoms in asthmatic patients. The Der p 2 is a major allergen and the predominant source of causative respiratory disorders which induce airway inflammation and bronchial constriction in mite-allergic patients. Few studies evaluate the ameliorating effects of modified Liu-Wei-Di-Huang-Wan (modified LWDHW) on allergic asthma. Methods This study aimed to investigate the immunological mechanisms of modified LWDHW on the inhibitory effects of airway inflammation, signal transduction, inflammatory cytokine production, Th2 cell proliferation, and bronchial obstruction in Der p 2-induced asthmatic mice. Results At least ten active ingredients were contained in the formula of modified LWDHW- 1217A and 1217B. Results showed that the immunoglobulin generations (Der p 2 specific- IgE and IgG1), inflammatory cytokine productions (IL-5 and IL-13) in the Sera and BALF could be down-regulated, and the Th1-cytokine productions (IL-12 and IFN-γ) be increased after immunotherapy with modified LWDHW of 1217A or 1217B. The inflammatory cell infiltrations (macrophages, eosinophils, and neutrophils) in the airway and the expressions of TH2-related genes (IL4, IL5, and IL13), TH2-related transcription factor (GATA-3), and neutrophil chemotactic chemokine (IL8) in the lung tissue of asthmatic mice were significantly decreased after the immunotherapy. The Th1/Th2 polarization had been identified that the IL-4+/CD4+ T cells were downregulated and IFN-γ+/CD4+ T cells were increased. The airway hyperresponsiveness to methacholine inhalation of Penh values was significantly decreased in the treated groups. There were significant improvements in the bronchus histopathology after immunotherapy with 1217A or 1217B which were evaluated by tracheal thickness, inflammatory cell count, and tracheal rupture of mouse lung. Conclusions It revealed that 1217A or 1217B could regulate the immune responses and improve pulmonary function. Data suggests that modified LWDHW of 1217A or 1217B have the potential for use as a therapeutic modality for the treatment of mite allergen Der p 2-induced allergic asthma.

show abstract

Effects of Local Nasal Immunotherapy with FIP-fve Peptide and Denatured Tyrophagus putrescentiae for Storage Mite-Induced Airway Inflammation

Cited by 7 publications

References 74 publications

Immunomodulatory effects of modified Liu-Wei-Di-Huang-Wan Traditional Chinese medicine on allergic asthmatic mice

Immunomodulatory effects of modified Liu-Wei-Di-Huang-Wan Traditional Chinese medicine on allergic asthmatic mice

The Present and Future of Allergen Immunotherapy in Personalized Medicine

Effects of traditional Chinese medicine on allergic asthmatic mice: using a combined formula

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