Effects of locally applied Clinoptilolite (Froximun®) on seroma formation in a rat mastectomy and axillary lymph node dissection model

Abstract

Objective: Seroma formation is one of the most frequent complications following breast cancer surgery. Although many techniques and drugs have been investigated, no definitive data are available concerning how to prevent this complication. The present study aims to investigate impact of local clinoptilolite application on seroma formation in an experimental mastectomy model created in rats. Material and Method: Two groups with seven rats in each were randomised. Under general anesthesia, all rats underwent right mastectomy and axillary dissection. Clinoptilolite was locally applied to surgical sites in the study group, whereas no application was performed in the control group. Ten days after the surgery, seroma formation was evaluated and tissue samples were obtained from the surgical sites, for both biochemical and histopathological examination Results: There was statistically significant decrease in seroma formation after clinoptilolite application (p=0.002). In addition, granulation tissue formation was significantly higher in the study group (p=0.006). No statistical difference was found between groups in terms of biochemical parameters. Conclusion: The reducing effect of seroma formation by clinoptilolite application may be due to its crystal cage structure. It collects seroma with its sero-absorbent features and creates granuloma formation that reduces dead-space, therefore accelarating wound healing.

The evaluation of the effects of natural zeolite (Clinoptilolite) in diabetic rats on bone healing in dental extracting socket

“… 28 It has been reported that macrophages and fibroblasts gradually increase in number in the early stage of the healing process and reach their highest level and gradually decrease as the healing process progresses, and the antioxidant effect of clinoptilolite occurs at this early stage. 21 Garcia Godoy F. et al 29 reported that clinoptilolite added locally to the graft in the bone defect created in the mandible, similar to our results, provided positive effects on bone regeneration in the early period. In addition, they showed that there was no significant difference between the groups in terms of healing and bone formation in the late 30 and 60 days.…”

“…Aksoy S.O. et al 21 applied Froximun Toxaprevent Skin powder® in powder form according to the wound size after mastectomy and axillary dissection in rats, and closed the wound area with sutures after local application. Uraloğlu M. et al 22 applied Froximun Toxaprevent Skin powder® to the wounds opened on the back skin of the rats locally, in powder form and according to the wound size, and did not suture the wounds.…”

“…Aksoy S.O. et al 21 attributed the mechanism of reducing seroma formation and accelerating wound healing of clinoptilolite (Froximun®) to the fact that clinoptilolite exerts a detoxification effect thanks to its absorbing structure with micropores, attracting the seroma with inflammatory exudate into its structure and increasing the formation of granulation tissue.…”

Sıçanlarda Mastektomi ve Aksiller Diseksiyon Sonrası Seroma Gelişimi Üzerine Kriyoprezerve İnsan Amniyotik Membran ve Trombositten Zengin Plazmanın Etkileri

“…The majority of these studies; it is designed on the basis of reducing the surgical cavity area, shortening the inflammatory process, and accelerating healing. In this context, different surgical techniques, pressure dressings, techniques that provide immobilization, tissue adhesives, and different agents have been involved in studies to prevent this complication (19,20). Platelets have an important role in hemostasis, tissue regeneration, and host defense.…”