Effects of long-term heavy metal contamination on soil microbial characteristics

Oliveira, A.; Pampulha, M. E.

doi:10.1263/jbb.102.157

Cited by 285 publications

(153 citation statements)

References 32 publications

Supporting

Mentioning

138

Contrasting

Unclassified

Order By: Relevance

“…It means that the culturable heterotrophic bacteria were more sensitive than other microbes. These findings confirm the results obtained by Oliveira and Pampulha (2006). In the studied microbes, the sensitivity was observed in order of fungi<actinomycetes<bacteria.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Effects of Cd and Pb on soil microbial community structure and activities

Khan

Hesham

Qiao

et al. 2009

Environ Sci Pollut Res

340

132

View full text Add to dashboard Cite

Background, aim, and scope Soil contamination with heavy metals occurs as a result of both anthropogenic and natural activities. Heavy metals could have long-term hazardous impacts on the health of soil ecosystems and adverse influences on soil biological processes. Soil enzymatic activities are recognized as sensors towards any natural and anthropogenic disturbance occurring in the soil ecosystem. Similarly, microbial biomass carbon (MBC) is also considered as one of the important soil biological activities frequently influenced by heavy metal contamination. The polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) has recently been used to investigate changes in soil microbial community composition in response to environmental stresses. Soil microbial community structure and activities are difficult to elucidate using single monitoring approach; therefore, for a better insight and complete depiction of the soil microbial situation, different approaches need to be used. This study was conducted in a greenhouse for a period of 12 weeks to evaluate the changes in indigenous microbial community structure and activities in the soil amended with different application rates of Cd, Pb, and Cd/Pb mix. In a field environment, soil is contaminated with single or mixed heavy metals; so that, in this research, we used the selected metals in both single and mixed forms at different application rates and investigated their toxic effects on microbial community structure and activities, using soil enzyme assays, plate counting, and advanced molecular DGGE technique. Soil microbial activities, including acid phosphatase (ACP), urease (URE), and MBC, and microbial community structure were studied. Materials and methods A soil sample (0-20 cm) with an unknown history of heavy metal contamination was collected and amended with Cd, Pb, and Cd/Pb mix using the CdSO 4 and Pb(NO 3 ) 2 solutions at different application rates. The amended soils were incubated in the greenhouse at 25±4°C and 60% water-holding capacity for 12 weeks. During the incubation period, samples were collected from each pot at 0, 2, 9, and 12 weeks for enzyme assays, MBC, numeration of microbes, and DNA extraction. Fumigationextraction method was used to measure the MBC, while plate counting techniques were used to numerate viable heterotrophic bacteria, fungi, and actinomycetes. Soil DNAs were extracted from the samples and used for DGGE analysis. Results ACP, URE, and MBC activities of microbial community were significantly lower (p <0.05) in the metal-amended samples than those in the control. The enzyme inhibition extent was obvious between different incubation periods and varied as the incubation proceeded, and the highest rate was detected in the samples after 2 weeks. However, the lowest values of ACP and URE activities (35.6% and 36.6% of the control, respectively) were found in the Cd 3 /Pb 3 -treated sample after 2 weeks. Similarly, MBC was strongly decreased in both Cd/Pbamended samples and highest reduction (52.4%) was Envir...

show abstract

Section: Discussionsupporting

confidence: 91%

“…These techniques have recently been used to investigate changes in soil microbial community composition (Claudia et al 2003;Oliveira and Pampulha 2006;Wang et al 2008;Moreels et al 2008). The DGGE technique is used for screening microbial community composition and also changes in response to environmental stresses (Mette and Neils 2002).…”

mentioning

confidence: 99%

Effects of Cd and Pb on soil microbial community structure and activities

Khan

Hesham

Qiao

et al. 2009

Environ Sci Pollut Res

340

132

View full text Add to dashboard Cite

show abstract

“…Other tests are connected with soil enzymatic activities, and, in the literature, the dehydrogenase activity (DHA) assay is mostly recommended (Oliveira and Pampulha, 2006;Swędrzyńska and Grześ 2015;Wolińska et al, 2013Wolińska et al, , 2014. These authors note that the DHA assay is a sensitive assay for determining the effect of heavy metals on physiologically active soil microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Community-level physiological profiles of microorganisms inhabiting soil contaminated with heavy metals

Kuźniar¹,

Banach²,

Stępniewska³

et al. 2018

International Agrophysics

View full text Add to dashboard Cite

A b s t r a c t. The aim of the study was to assess the differences in the bacterial community physiological profiles in soils contaminated with heavy metals versus soils without metal contaminations. The study's contaminated soil originated from the surrounding area of the Szopienice non-ferrous metal smelter (Silesia Region, Poland). The control was soil unexposed to heavy metals. Metal concentration was appraised by flame atomic absorption spectrometry, whereas the the community-level physiological profile was determined with the Biolog EcoPlates TM system. The soil microbiological activity in both sites was also assessed via dehydrogenase activity. The mean concentrations of metals (Cd and Zn) in contaminated soil samples were in a range from 147.27 to 12265.42 mg kg -1 , and the heavy metal contamination brought about a situation where dehydrogenase activity inhibition was observed mostly in the soil surface layers. Our results demonstrated that there is diversity in the physiological profiles of microorganisms inhabiting contaminated and colntrol soils; therefore, for assessment purposes, these were treated as two clusters. Cluster I included colntrol soil samples in which microbial communities utilised most of the available substrates. Cluster II incorporated contaminated soil samples in which a smaller number of the tested substrates was utilised by the contained microorganisms. The physiological profiles of microorganisms inhabiting the contaminated and the colntrol soils are distinctly different.K e y w o r d s: Biolog ECO plates, community-level physiological profiles, heavy metals

show abstract

“…The man-made wastes including automobile wastes introduced into the soil environment amplify the magnitude of the trace element almost to an extent of threatening life and entire ecosystem (Oliveira and Pampulha, 2006). In many cases, the wastes are taken up by roots and shoot of plants and in turn gets into other higher organisms including man when ingested (KabataPendias, 2002).…”

Section: Introductionmentioning

confidence: 99%

Effects of automobile battery wastes on physicochemical properties of soil in Benin City, Edo State

Orjiakor¹,

Atuanya²

2015

Glo Jnl Pure Appl Sci

View full text Add to dashboard Cite

Difference in soil qualities has been noticeable in many soils due to anthropogenic sources, especially of automobile battery wastes. This study examines the effects of automobile battery wastes on the physicochemical properties of the soil. Soil samples for this study were collected in triplicates from three battery chargers' workshops: Adolor, Edaiken and Uwelu in Benin City, Edo State at 0-15cm depth, in the months of August, September and October. The soil physicochemical parameters analyzed indicate variations of values in the contaminated soil over uncontaminated soil (control). Among the parameters examined, conductivity was significantly (P<0.01) higher in the contaminated soil (59.3-184mho/cm) than in the uncontaminated soil. Notably, a more acidic pH value of 3.7-4.5 was also recorded beyond standard limits of 6.5~8.5. Meanwhile phosphorus was relatively high (1.95-3.35) and nitrogen (0.08-0.15) was low as against the control value of 2.71 and 0.18 respectively. Heavy metals such as Lead (Pb), Zinc (Zn), Cadmium (Cd), Chromium (Cr) and Copper (Cu) were present in different concentrations in contaminated soil sample which ranges from far above acceptable standard limit between (0.288-0.875, 0.757-1.342, 0.108-0.279, 0.718-1.062 and 0.272-0.518 mg/kg) compared to their values in the control soil sample having 0.003, 0.125, 0, 0 and 0.063 mg/kg respectively. Battery wastes were found to be significant sources of Cadmium and Chromium, as none of both was detected in the control soil sample. The daily activities of auto-mechanic battery workshops have negative impacts on soil physicochemical properties. Note, the soil in mechanic battery workshops needs urgent cleanup to minimize contamination of ecological materials and public health implication. This work will prove valuable in providing baseline information for further soil quality monitoring studies in study area

show abstract

Effects of long-term heavy metal contamination on soil microbial characteristics

Cited by 285 publications

References 32 publications

Effects of Cd and Pb on soil microbial community structure and activities

Effects of Cd and Pb on soil microbial community structure and activities

Community-level physiological profiles of microorganisms inhabiting soil contaminated with heavy metals

Effects of automobile battery wastes on physicochemical properties of soil in Benin City, Edo State

Contact Info

Product

Resources

About