Effects of long-term nitrate supplementation on carbohydrate metabolism, lipid profiles, oxidative stress, and inflammation in male obese type 2 diabetic rats

Gheibi, Sevda; Jeddi, Sajad; Carlström, Mattias; Gholami, Hanieh; Ghasemi, Asghar

doi:10.1016/j.niox.2018.02.002

Cited by 72 publications

(65 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Contradictory to our findings, Gheibi et al. () have reported a decreased body weight in both control and diabetic rats following 100 mg l −1 sodium nitrate administration for 2 months in type 2 diabetic rats. However, no changes in body weight were reported after 60 days’ administration of sodium nitrate (200 and 400 mg l −1 ) in adult rats (Ogur et al., ) or potassium nitrate (4% of dry feed) in lambs (Cline, Hatfield, & Garrigus, ), 14 weeks’ sodium nitrate treatment (375 and 750 mg l −1 ) in rats (National Toxicology Program, ), and 13 weeks’ potassium nitrate treatment (100 mg l −1 ) in both male and female rats (Til, Kuper, & Falke, ).…”

Section: Discussionsupporting

confidence: 72%

“…Nitrate supplementation and insulin therapy restored all three indexes. According to previous findings, this effect of nitrate is probably related to its antioxidant and anti‐hyperglycaemic activity (Bahadoran et al., ; Gheibi et al., ).…”

Section: Discussionmentioning

confidence: 69%

“…However, Gheibi et al. () have reported that chronic nitrate administration in obese type 2 diabetic rats reduced serum insulin level. Furthermore, Nyström et al.…”

Section: Discussionmentioning

confidence: 98%

“…Increase in nitrite and NOx following nitrate supplementation is importantly dependent on commensal bacteria in the mouth, pH in the stomach and functional xanthine oxidoreductase activity (Lundberg & Weitzberg, ; Montenegro et al., ; Peleli et al., ). One study showed increased serum NOx levels following nitrate supplementation in diabetic rats after 8 weeks (Khalifi et al., ); in another study, serum nitrate levels on day 42 in nitrate‐treated diabetic rats were lower than in nitrate‐treated controls (Gheibi et al., ). Although this observation cannot explain the results of this study, it may be dependent on duration of administration.…”

Section: Discussionmentioning

confidence: 99%

“…There have been some studies on the effects of nitrate therapy on glucose‐stimulated insulin secretion and pancreatic islet insulin content (Gheibi et al., ). Furthermore, nitrate supplementation via drinking water reduced glucose intolerance in endothelial nitric oxide synthase‐deficient mice (Carlström et al., ) and also decreased insulin resistance and sensitivity in type 2 diabetic rats (Gheibi, Jeddi, Carlström, Gholami, & Ghasemi, ). A systematic review by Jackson et al .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Oghbaei

Alipour

Hamidian

et al. 2018

Experimental Physiology

View full text Add to dashboard Cite

Inorganic nitrate supplementation is a possible therapeutic agent in diabetes. The aim of this study was to evaluate the effect of nitrate on the reproductive system in streptozotocin-induced diabetic male rats. Fifty male Wistar rats were allocated randomly to five groups: control (C), control plus nitrate (CN), diabetic (D), diabetic plus insulin (DI) and diabetic plus nitrate (DN). Sodium nitrate was administered for 2 months in the drinking water (100 mg l ) of the CN and DN groups. Insulin was injected at 2-4 U daily in the DI group. Serum glucose level and body weight were measured at the beginning of the study and at regular intervals. At the end of the study, serum levels of glucose, insulin, nitrogen oxides (NOx), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were assessed as well as sperm parameters, testis morphometry and histology, and testicular miR-34b and p53 mRNA expression. Nitrate treatment in diabetic rats significantly improved sperm parameters, epididymal weight, spermatogenesis and testicular histology as well as decreasing serum glucose and testicular p53 gene and miR-34b expression, although it had no effect on serum LH and FSH levels. In diabetic rats, serum insulin and NOx, body weight, testicular and epididymal weight, sperm count and motility, testis morphology, spermatogenesis indices, Johnsen's score, and testosterone were significantly lower than in controls. Nitrate administration increased serum insulin, NOx and testosterone levels in the DN group. Consuming water supplemented with sodium nitrate could improve diabetes-induced testicular functional and structural disorders; these favourable effects may be related to increased serum insulin and decreased serum glucose, as well as modulation of apoptosis in testis.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 69%

“…However, Gheibi et al. () have reported that chronic nitrate administration in obese type 2 diabetic rats reduced serum insulin level. Furthermore, Nyström et al.…”

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Oghbaei

Alipour

Hamidian

et al. 2018

Experimental Physiology

View full text Add to dashboard Cite

show abstract

Reactive Oxygen Species, Glucose Metabolism, and Lipid Metabolism

Yang

Sun

et al. 2021

Oxidative Stress

View full text Add to dashboard Cite

Long-term dietary nitrate supplementation slows the progression of established atherosclerosis in ApoE−/− mice fed a high fat diet

et al. 2023

View full text Add to dashboard Cite

Background and aims Atherosclerosis is associated with a reduction in the bioavailability and/or bioactivity of endogenous nitric oxide (NO). Dietary nitrate has been proposed as an alternate source when endogenous NO production is reduced. Our previous study demonstrated a protective effect of dietary nitrate on the development of atherosclerosis in the apoE−/− mouse model. However most patients do not present clinically until well after the disease is established. The aims of this study were to determine whether chronic dietary nitrate supplementation can prevent or reverse the progression of atherosclerosis after disease is already established, as well as to explore the underlying mechanism of these cardiovascular protective effects. Methods 60 apoE−/− mice were given a high fat diet (HFD) for 12 weeks to allow for the development of atherosclerosis. The mice were then randomized to (i) control group (HFD + 1 mmol/kg/day NaCl), (ii) moderate-dose group (HFD +1 mmol/kg/day NaNO3), or (iii) high-dose group (HFD + 10 mmol/kg/day NaNO3) (20/group) for a further 12 weeks. A group of apoE−/− mice (n = 20) consumed a normal laboratory chow diet for 24 weeks and were included as a reference group. Results Long-term supplementation with high dose nitrate resulted in ~ 50% reduction in plaque lesion area. Collagen expression and smooth muscle accumulation were increased, and lipid deposition and macrophage accumulation were reduced within atherosclerotic plaques of mice supplemented with high dose nitrate. These changes were associated with an increase in nitrite reductase as well as activation of the endogenous eNOS-NO pathway. Conclusion Long-term high dose nitrate significantly attenuated the progression of established atherosclerosis in the apoE−/− mice fed a HFD. This appears to be mediated in part through a XOR-dependent reduction of nitrate to NO, as well as enhanced eNOS activation via increased Akt and eNOS phosphorylation.

show abstract

Effects of long-term nitrate supplementation on carbohydrate metabolism, lipid profiles, oxidative stress, and inflammation in male obese type 2 diabetic rats

Cited by 72 publications

References 79 publications

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Reactive Oxygen Species, Glucose Metabolism, and Lipid Metabolism

Long-term dietary nitrate supplementation slows the progression of established atherosclerosis in ApoE−/− mice fed a high fat diet

Contact Info

Product

Resources

About