Effects of Low Concentrations of Nitric Oxide on the Zona Pellucida Binding Ability of Human Spermatozoa

Sengoku, Kazuo; Tamate, Kenichi; Yoshida, Toshiaki; Takaoka, Yasuo; Miyamoto, Toshinobu; Ishikawa, Mutsuo

doi:10.1016/s0015-0282(97)00537-2

Cited by 84 publications

(55 citation statements)

References 31 publications

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“…Three NOS isoforms have been described: endothelial (eNOS, NOS3), neuronal (nNOS, NOS1), and inducible (iNOS or NOS2; Nathan & Xie 1994). NO has been demonstrated to play a role in a variety of functions in the human reproductive tract, including sperm motility (Lewis et al 1996), chemotaxis (Miraglia et al 2007), and sperm-zona pellucida binding ability (Sengoku et al 1998). NOS isoforms have been localized in the acrosome and tail of human, mouse, and bovine spermatozoa (Herrero et al 1996, Meiser & Schulz 2003, and low motility spermatozoa have been shown to exhibit aberrant patterns of NOS3 immunostaining (O'Bryan et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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Nitric oxide (NO), a modulator of several physiological processes, is involved in different human sperm functions. We have investigated whether NO may stimulate the motility of human spermatozoa via activation of the soluble guanylate cyclase (sGC)/cGMP pathway. Sperm samples obtained by masturbation from 70 normozoospermic patients were processed by the swim-up technique. The kinetic parameters of the motile sperm-rich fractions were assessed by computer-assisted sperm analysis. After a 30-90 min incubation, the NO donor S-nitrosoglutathione (GSNO) exerted a significant enhancing effect on progressive motility (77, 78, and 78% vs 66, 65, and 62% of the control at the corresponding time), straight linear velocity (44, 49, and 48 mm/s vs 34, 35, and 35.5 mm/s), curvilinear velocity (81, 83, and 84 mm/s vs 68 mm/s), and average path velocity (52, 57, and 54 mm/s vs 40, 42, and 42 mm/s) at 5 mM but not at lower concentrations, and in parallel increased the synthesis of cGMP. A similar effect was obtained with the NO donor spermine NONOate after 30 and 60 min. The GSNO-induced effects on sperm motility were abolished by 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (a specific sGC inhibitor) and mimicked by 8-bromo-cGMP (8-Br-cGMP; a cell-permeating cGMP analog); the treatment with Rp-8-Br-cGMPS (an inhibitor of cGMP-dependent protein kinases) prevented both the GSNO-and the 8-Br-cGMP-induced responses. On the contrary, we did not observe any effect of the cGMP/PRKG1 (PKG) pathway modulators on the onset of hyperactivated sperm motility. Our results suggest that NO stimulates human sperm motility via the activation of sGC, the subsequent synthesis of cGMP, and the activation of cGMP-dependent protein kinases.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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“…NO production is *Address correspondence to this author at the Department of Physiology, College of Medicine, P/Bag 360, Blantyre 3, Malawi; Tel: +265-(0) 1 871911; Fax: +265-(0) 1 872644; E-mail: flampiao@medcol.mw catalyzed by a family of NO synthase (NOS) enzymes [13] and has been shown to be expressed in human spermatozoa [14]. Studies have shown that exogenously applied NO had different effects on sperm function depending on the concentrations applied [15,16]. We have previously shown that intracellular NO in human spermatozoa can be detected and measured by flow cytometry [17].…”

Section: Introductionmentioning

confidence: 99%

Effects of Sperm Processing Techniques Involving Centrifugation on Nitric Oxide, Reactive Oxygen Species Generation and Sperm Function~!2009-11-11~!2010-01-07~!2010-03-30~!

Lampiao¹,

Strijdom²,

Plessis³

2010

TOANDROJ

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This study was aimed at investigating the effects of sperm centrifugation on nitric oxide (NO) and reactive oxygen species (ROS) generation as well as sperm motility and viability. Human spermatozoa were centrifuged for 10 and 30 minutes (400 x g) in the presence or absence of the NOS inhibitor, N G -nitro-L-arginine methyl ester (L-NAME); ROS scavenger, N-(2-mercaptopropionyl)Glycine (MPG) or the combination of L-NAME + MPG. Total sperm motility was significantly decreased with 30 minutes of centrifugation whereas progressive motility and cell viability were significantly decreased with 10 and 30 minutes of centrifugation. These effects were reversed with the administration of MPG or L-NAME + MPG. Ten minutes centrifugation significantly elevated ROS and NO production (P < 0.05). Thirty minutes centrifugation elevated ROS generation (P < 0.01) whereas NO was attenuated. This study has demonstrated that 10 and 30 minutes of sperm centrifugation were detrimental to both sperm motility and viability, but generally 30 minutes centrifugation was more detrimental to sperm than 10 minutes. It has also demonstrated that 10 minutes centrifugation led to both NO and ROS elevation whereas 30 minutes centrifugation led to ROS elevation and NO attenuation. We therefore recommend that sperm separation techniques should avoid using centrifugation or prolonged centrifugation in assisted reproductive technologies.

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“…Some reports indicate that NOS may be involved in the normal biosynthesis and secretion of the steroid hormones in the male reproductive system, demonstrating direct effects on the function of interstitial cells in the testes [21,25]. On the other hand, excessive NOS may induce the production of large amounts of NO metabolites in response to a variety of stressors, possibly reducing the survival rate and motility of sperm cells [18,20].…”

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confidence: 99%

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Ha¹,

Kim

Shin

2004

The Journal of Veterinary Medical Science

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ABSTRACT. The expression of three isoforms of nitric oxide synthase (NOS) were examined in the testis and epididymis of a thoroughbred horse. Immunohistochemical studies demonstrated the presence of eNOS immunostaining in some germ cells in the seminiferous tubules and in vascular endothelial cells in the interstitial tissues. Interstitial cells, most likely Leydig cells, were also intensel y immunopositive for eNOS. The pattern of immunostaining for nNOS was similar to that for eNOS in the testis. Weak expression of iNOS was detec ted in the seminiferous tubules of the testis, but intense expression was found in interstitial cells. Inducible NOS was also stron gly detected in stereocilia, sperm, epithelium and connective tissue of the epididymis of normal horses. These findings suggest that three isoforms of NOS are expressed in the testis and epididymis of horse and that they play important roles in the biology of interstitial cells that produce testosterone, as well as in spermatogenesis in the seminiferous tubules. KEY WORDS: epididymis, equine, nitric oxide synthase, testis.

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Effects of Low Concentrations of Nitric Oxide on the Zona Pellucida Binding Ability of Human Spermatozoa

Cited by 84 publications

References 31 publications

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Effects of Sperm Processing Techniques Involving Centrifugation on Nitric Oxide, Reactive Oxygen Species Generation and Sperm Function~!2009-11-11~!2010-01-07~!2010-03-30~!

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

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