Effects of Low-Density Lipoprotein from Normal Rabbits on Hypercholesterolemia and the Development of Atherosclerosis

Abstract

To clarify whether or not normal LDL separated from normal rabbits is atherogenic, 0.75 mg of normal LDL-cholesterol in 6 ml of 0.85% saline and 6 ml of saline alone were injected every day for 5 weeks into the auricular vein of two groups of rabbits, respectively. The inoculated rabbits were fed a standard diet containing 0.5 and 1 % cholesterol. Blood was drawn before injection and at 1, 2, 3 and 5 week intervals thereafter. After 5 weeks, all rabbits were sacrificed. Following exsanguination, the aorta was stained by Sudan III. The Sudan III staining was more extensive in rabbits injected with saline than in those that received LDL. In addition, plasma cholesterol, plasma phospholipid, VLDL-cholesterol and LDL-cholesterol levels were lower in LDL-injected rabbits than in those injected with saline. But HDL-cholesterol levels were not significantly different between LDL-cholesterol and saline-injected rabbits. Although the exact cause of antiatherogenic effect of normal LDL is not clear, it seems reasonable to suggest that at least some normal LDL acts as a ‘good’ lipoprotein, namely antiatherogenic lipoprotein, in our experimental protocol.

Inhibitory effect of co‐administration of atorvastatin and endothelin‐1 receptor antagonist on the progression of atherosclerosis in rabbit

“…Rabbit aortas were stained with Sudan III staining (Yilian Biotechnology, Shanghai, China) as per the manufacturer's instructions to visualize atherosclerotic lesions . Briefly, the aorta was stained with Sudan III dye solution (0.5% Sudan III in 35% ethanol and 50% acetone saturated solution) for 15 min, before being washed with 80% ethanol and double distilled water and finally fixed in 4% paraformaldehyde .…”

“…Rabbit aortas were stained with Sudan III staining (Yilian Biotechnology, Shanghai, China) as per the manufacturer's instructions to visualize atherosclerotic lesions . Briefly, the aorta was stained with Sudan III dye solution (0.5% Sudan III in 35% ethanol and 50% acetone saturated solution) for 15 min, before being washed with 80% ethanol and double distilled water and finally fixed in 4% paraformaldehyde . Images were obtained using a scanning electron microscope interfaced with a CCD camera (Nikon, Tokyo, Japan), and were analyzed using scanning electron microscope image processing system (SEM‐IPS) (Kontron Imaging System, Kontron Elektronik GmbH, München, Germany) to measure the thickness and area of the intima‐media as well as the intima/media area ratio by an investigator blinded to the experimental protocol.…”

Batroxobin Accelerates Lipid Accumulation of Peroxidized Low Density Lipoprotein in Mouse Peritoneal Macrophages

“…3], accelerate platelet aggregability and injure the endothelium of the artery [4][5][6][7], These findings suggest that in treating ath erosclerosis, emphasis should be placed on preventing the peroxidization of LDL and eliminating the peroxidized LDL (pox-LDL).…”