Effects of Low dose Aroclor 1254 on Few Lysosomal Biomarker Enzymes from Liver and Brain of Mice under in vitro condition

Abstract

Aroclor 1254 contains very high concentration of chorine which makes it a persistent and toxic pollutant in the environment. The references of its toxic effects on liver and brain lysosomal biomarker enzymes are not abundantly available. Present study reports the possible dose and duration dependent toxic effects of low dose of Aroclor 1254 on few extracted lysosomal biomarker enzymes, acid phosphatase, αgalactosidase, β-galactosidase and β-glucuronidase from liver and brain tissues of mice under in vitro condition. Two hypotheses were tested in the present study, (a) Aroclor 1254 has a dose and duration dependent effects on the activities of selected extracted enzymes and (b) the response exhibits an organ specific effect of the toxicant. The results revealed both significant exposure duration dependent and organ specific effects on the activities of lysosomal enzymes. The observed results indicated that if Aroclor 1254 enters into the cell and comes in direct contact with the lysosomal enzymes, it may influence the activities of the lysosome in a complex, organ specific way. Though the exact nature of the influences at cellular level is not clear, these may lead to an increase in the cellular accumulation of toxic substances which may contribute adverse toxic effects on the gross physiological conditions of the cell and the organism.