Effects of Low Dose Dioxin on Membrane Bound Ion Dependent ATPases in Mice Kidney

Jigyasi, Jyoti

doi:10.9790/2402-0254348

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…Therefore, one of the possibilities of indirect effect of Aroclor 1254 is oxidative stress which may alter the activity of membrane bound ion dependent ATPases by producing free radicals. It may also be possible that PCBs accumulated in the intracellular space and alter the activity of membrane bound ion-dependent ATPases which altered the membrane permeability [21][22][23][24].…”

Section: Resultsmentioning

confidence: 99%

Low Concentrations of PCB (Aroclor 1254) Alter Membrane Bound Ion Dependent ATPases in the Hepatocyte Cells of Mice

Pathak¹

2013

IOSR-JESTFT

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Aroclor 1254, a PCB, notwithstanding its banned status, is still available in the environment in low concentration. Reports on its toxic effects on membrane proteins and the mechanism of actions are rare. Therefore, the present study was conducted to investigate the dose and exposure duration dependent effects of low concentration Aroclor 1254 on total, Na + , K + , Ca ++ and Mg ++ -ATPases on the mouse liver cells. The study tests two hypotheses, (a) the low, environmentally available doses of Aroclor 1254 will cause typical dose and exposure duration dependent responses on the membrane-bound ion dependent ATPases of the hepatic cells and (b) the observed response will be a direct effect of Aroclor on the enzyme protein itself. To check these hypotheses, various groups of mice were exposed to two sublethal doses for three exposure durations of Aroclor 1254. The results indicated no dose dependent effects on the enzymes. However, significant exposure duration dependent changes in membrane bound ATPases were observed. Since the observed changes were mostly enzyme stimulation after subjected to toxication through oral route, the effects of the Aroclor were possibly in the form of indirect oxidative stress through complex chain of reactions exhibited by the cells.

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Section: Resultsmentioning

confidence: 99%

Low Concentrations of PCB (Aroclor 1254) Alter Membrane Bound Ion Dependent ATPases in the Hepatocyte Cells of Mice

Pathak¹

2013

IOSR-JESTFT

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“…The fact that, the current contributed by N a+ is of unexpectedly of the outward type, could be explained hypothesizing that the Na + current activates a pumping system that pumps out a quantity of current significantly larger than the inward current produced by the ionic channels. Pumps sensitive to local ionic changes of concentrations have been largely described [31] and complex transport systems have been also identified in a cell line similar to ours [32][33][34][35]. However, these results are preliminary and, of course, need of further confirmations to be supported.…”

Section: Another Observation By Considering the Results Of Solution Cmentioning

confidence: 60%

Membrane Currents in Madin-Darby Bovine Kidney Cells are Enhanced by Exposure to Dioxin

Santillo

Fiorito

Martino

2019

BJSTR

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“…ATPases are the membrane-bound enzymes that are mainly involved in the regulation of the ions and metabolites across the plasma membrane in order to maintain the normal physiological and biochemical functions of cells. 43 They require thiol groups and phospholipids to maintain their structure and function. 44 Any alterations in membrane lipids lead to changes in membrane uidity, which in turn, disturbs the membrane- bound enzyme activities, including total ATPases, Mg 2+ ATPase, Ca 2+ ATPase, and Na + K + ATPase and cellular functions.…”

Section: Discussionmentioning

confidence: 99%

An experimental study to investigate the impact of p-coumaric acid, a common dietary polyphenol, on cadmium chloride-induced renal toxicity

Navaneethan

Rasool

2014

Food Funct.

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Cadmium, a well-known environmental pollutant and a toxic transitional metal causes severe damage to many organs, such as liver, kidney, lungs, heart, etc. The current study has been designed to assess the impact of p-coumaric acid, a common dietary polyphenol on cadmium chloride-induced renal toxicity in rats. Therefore, the activities of membrane bound ATPases, mitochondrial TCA cycle and electron transport chain enzymes, gluconeogenic and glycolytic enzymes, and glycogen content were estimated in kidney tissue homogenates of control and experimental rats. In addition, the serum levels of glucose and pro-inflammatory cytokines, such as TNF-α and IL-1β were also estimated. The cadmium chloride administered rats (3 mg per kg per b. wt per s.c.) showed significant decrease in the levels of membrane bound ATPases, mitochondrial TCA cycle and electron transport chain enzymes, glycolytic enzymes, and glycogen content as compared with controls. Conversely, the levels of glucose, gluconeogenic enzymes and pro-inflammatory cytokines (TNF-α, and IL-1β) were found to be increased. However, the administration of p-coumaric acid (100 mg per kg per b. wt per s.c.) along with the cadmium chloride significantly modulated these biochemical and immunological changes to near normal, as compared to cadmium chloride treated rats. Thus, the results provide strong evidence that p-coumaric acid has a protective action against cadmium-induced renal toxicity in rats.

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Effects of Low Dose Dioxin on Membrane Bound Ion Dependent ATPases in Mice Kidney

Abstract: The present communication reports the dose and exposure dependent effects of a dioxin on few ion dependent membrane bound ATPases of mice kidney. Effects of TCDD on the specific activity of few membrane bound ATPases viz.

Cited by 7 publications

References 22 publications

Low Concentrations of PCB (Aroclor 1254) Alter Membrane Bound Ion Dependent ATPases in the Hepatocyte Cells of Mice

Low Concentrations of PCB (Aroclor 1254) Alter Membrane Bound Ion Dependent ATPases in the Hepatocyte Cells of Mice

Membrane Currents in Madin-Darby Bovine Kidney Cells are Enhanced by Exposure to Dioxin

An experimental study to investigate the impact of p-coumaric acid, a common dietary polyphenol, on cadmium chloride-induced renal toxicity

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