Effects of Low‐Dose Doxycycline and Bisphosphonate Clodronate on Alveolar Bone Loss and Gingival Levels of Matrix Metalloproteinase‐9 and Interleukin‐1β in Rats With Diabetes: A Histomorphometric and Immunohistochemical Study

Ozdemir, Selin; Kurtiş, Bülent; Tüter, Gülay; Bozkurt, Şeyma Büşra; Gültekin, Sibel Elif; Şengüven, Burcu; Watanabe, Keiko; Aydın, Sevtap

doi:10.1902/jop.2012.110459

Cited by 27 publications

(32 citation statements)

References 64 publications

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“…This result might be attributable to the anti-collagenolytic effect of doxycycline, which enhances the bone-forming ability via osteoblast cell chemotaxis and reduces bone resorption via osteoclasts (34). In vivo periodontal studies have shown that combined therapy with alendronate and/or doxycycline leads to a reduction in hard tissue destruction, inhibition and down-regulation of matrix metalloproteinase (8,9).…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Local Delivery Doxycycline and Alendronate on Bone Repair

et al. 2015

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Abstract. The aim of the present study was to investigate the local effect of 10% doxycycline and 1% alendronate combined with poly(lactic-co-glycolic acid) (PLGA) on bone repair. Thirty rats were divided into three groups, as follows: control group (CG), drug group (DG), and vehicle-PLGA group (VG). Bone defect was created in the right femur and filled with the following: blood clot (CG); PLGA gel, 10% doxycycline and 1% alendronate (DG); or vehicle-PLGA (VG). The animals were euthanized 7 or 15 days after surgery. Bone density, bone matrix and number of osteoclasts were quantified. At 7 days, the findings showed increased density in DG (177.75±76.5) compared with CG (80.37±27.4), but no difference compared with VG (147.1±41.5); no statistical difference in bone neoformation CG (25.6±4.8), VG (27.8±4), and DG (18.9±7.8); and decrease osteoclasts in DG (4.6±1.9) compared with CG (26.7±7.4) and VG (17.3±2.7). At 15 days, DG (405.1±63.1) presented higher density than CG (213.2±60.9) and VG (283.4±85.8); there was a significant increase in percentage of bone neoformation in DG (31.5±4.2) compared with CG (23 ±4), but no difference compared with VG (25.1±2.9). There was a decreased number of osteoclasts in DG (20.7±4.7) and VG (29.5±5.4) compared with CG (40±9.4). The results suggest that the association of 10% doxycycline and 1% alendronate with PLGA-accelerated bone repair.

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Section: Discussionmentioning

confidence: 99%

“…Recent investigations have shown that different classes of drugs, such as bisphosphonates, tetracyclines, and their combination, stimulate osteogenesis when their use was evaluated with regard to alveolar bone loss in periodontitis (8)(9)(10), healing of bone defects (1) and in vitro cell culture (11,12).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Local Delivery Doxycycline and Alendronate on Bone Repair

et al. 2015

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“…The host response to these infectious pathogens leads to dispersal of inflammatory mediators, including the pro-inflammatory cytokines interleukin-1 (IL-1), IL-6, tumor necrosis factor alpha (TNF-α), and prostaglandins (PGE2), the latter exacerbating deterioration of the periodontium. The increased cytokine levels present in periodontitis are also likely to aggravate existing conditions, such as atherosclerosis, diabetes, and rheumatoid arthritis (3).increased likelihood and severity of periodontal disease in individuals with diabetes mellitus (DM) (4). It has been suggested that periodontitis could have a detrimental effect on DM control, as it has been noted that periodontal treatment can improve gylcemic status.…”

Section: Introductionmentioning

confidence: 99%

Levels of vaspin and omentin-1 in gingival crevicular fluid as potential markers of inflammation in patients with chronic periodontitis and type 2 diabetes mellitus

et al. 2016

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Abstract:The aims of the present study were to determine the levels of vaspin and omentin-1 in gingival crevicular fluid (GCF) in patients with chronic periodontitis (CP) with and without type 2 diabetes mellitus (T2DM), and to evaluate GCF vaspin and omentin-1 levels after non-surgical periodontal therapy. The study included 60 subjects: 15 systemically and periodontally healthy individuals, 15 periodontally healthy patients with T2DM, 15 systemically healthy patients with CP, and 15 patients with both CP and T2DM. GCF and clinical periodontal parameters were examined at the baseline and 6 weeks after periodontal therapy. Levels of vaspin, omentin-1 and tumor necrosis factor-alpha (TNF-α) were measured by ELISA, and their relative ratios were calculated. GCF vaspin and TNF-α levels were significantly higher in the CP groups than in the periodontally healthy groups (P < 0.008) and decreased after therapy in the former (P < 0.025). GCF omentin-1 levels were significantly lower in the CP groups than in the periodontally healthy groups (P < 0.008) and increased after therapy in the former (P < 0.05). Statistically significant positive correlations were found between the total amount of vaspin and TNF-α, glycated hemoglobin (HbA1c), clinical attachment level and gingival index, whereas the level of omentin-1 was negatively correlated with these parameters in all groups (P < 0.05). We found that non-surgical periodontal therapy influenced the GCF levels of both vaspin and omentin-1 in the CP groups. IntroductionChronic peridontitis (CP) is an infectious condition that degrades and deteriorates the vital periodontal tissues around the teeth (1). The deterioration of soft and hard tissues resulting from periodontitis is due to stimulation of the host immunoinflammatory reaction to pathogens in the mouth (2). The host response to these infectious pathogens leads to dispersal of inflammatory mediators, including the pro-inflammatory cytokines interleukin-1 (IL-1), IL-6, tumor necrosis factor alpha (TNF-α), and prostaglandins (PGE2), the latter exacerbating deterioration of the periodontium. The increased cytokine levels present in periodontitis are also likely to aggravate existing conditions, such as atherosclerosis, diabetes, and rheumatoid arthritis (3). A number of theories have been offered to explain the

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“…5 Many studies have long recognized that disease is common among diabetic patients and becomes worse with the progression of diabetes. 6,7 Several mechanisms have been reported to explain the greater incidence and severity of periodontal disease in patients with diabetes mellitus (DM). These include polymorphonuclear leukocyte (PMN) dysfunction, vascular changes, altered collagen and glycosaminoglycan synthesis, deregulated cytokine production, and the formation of advanced glycation end products (AGEs).…”

Section: öZmentioning

confidence: 99%