Effects of low-dose ionizing radiation on α,β-globulins solutions studied by DSC

Abstract

An attempt has been made to detect the effect of a small dose of ionizing radiation on the course of a,b-globulin thermal denaturation in aqueous solutions. Doses of 0.1 and 1.8 Gy have been delivered using c-rays emitted by 60 Co isotope while doses of 10 and 100 Gy have been supplied by X-rays produced by linear accelerator. The highest dose has visibly changed DSC curve of protein solution while the changes due to lower doses are hardly detectable. Although very weak, the irradiation effect found has been dose dependent. The results suggest that the influence of ionizing radiation on globulins solution is bigger when the dose rate is lower at given dose. The opposite direction of differences between irradiated and control samples for fresh and stored protein solutions suggests various characters of changes in initial and later period of sample aging. This may be an important reason for difficulties in an investigation of the effect of ionizing radiation on protein solution, especially for low doses delivered very slowly.

Reversing radiation-induced immunosuppression using a new therapeutic modality

“…Furthermore, recent studies have shown that there are other key players involved in radiation associated damage and targeting them using conventional approaches such as recombinant proteins or small molecule drugs is time consuming, laborious, expensive and ultimately not feasible. 2,3,[21][22][23][24][25][26][27][28][29][30]5,[11][12][13][14][18][19][20] To address the limitations of the existing approaches, we have developed a new approach to highthroughput drug-discovery, target validation, and lead molecule identification using nucleic acidbased molecules, called Nanoligomer™, that are rationally designed and synthesized to up-or downregulate expression of target gene at the messenger RNA (mRNA) or protein level. Furthermore, it alters related gene network targets ultimately resulting in pathway modulation.…”

Reversing Radiation-Induced Immunosuppression Using a New Therapeutic Modality

“…Furthermore, recent studies have shown that there are other key players involved in radiation associated damage and targeting them using conventional approaches such as recombinant proteins or small molecule drugs is time consuming, laborious, expensive and ultimately not feasible. 2,3,[21][22][23][24][25][26][27][28][29][30]5,[11][12][13][14][18][19][20] To address the limitations of the existing approaches, we have developed a new approach to highthroughput drug-discovery, target validation, and lead molecule identification using nucleic acidbased molecules, called Nanoligomer™, that are rationally designed and synthesized to up-or downregulate expression of target gene at the messenger RNA (mRNA) or protein level. Furthermore, it alters related gene network targets ultimately resulting in pathway modulation.…”

MiRNA expression profile of ionizing radiation‐induced liver injury in mouse using deep sequencing

“…With the development of nuclear energy and radiotherapy, IR is widely applied in many fields such as research, medicine, food industry, energy production, etc. (Michnik et al, ). In medical field, IR has been employed as a beneficial therapeutic tool to destroy cancer cells, but it can also damage normal cells and tissues with direct energy deposition or reactive free radicals generated (Seyed Jalal, ; Koturbash et al, ).…”