Effects of low doses of carbendazim or iprodione either separately or in mixture on the pubertal rat seminiferous epithelium: An ex vivo study

Durand, Philippe; Martin, Guillaume; Blondet, Antonine; Gilleron, Jérôme; Carette, Diane; Janczarski, Stéphane; Christin, Emilie; Pointis, Georges; Perrard, Marie-Hélène

doi:10.1016/j.tiv.2017.05.022

Cited by 22 publications

(15 citation statements)

References 49 publications

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“…Additionally, Durand et al. (2017) showed that the number of precursor cells in the spermatozoa of pubertal rats was reduced by IPR. IPR combined with human androgen receptors at a particular dose (200 mg IPR kg −1 day −1 postnatal day (PND) 23 to 55–57) was also relieved by Blystone et al.…”

Section: Introductionmentioning

confidence: 99%

Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic‐ and spermatogenic‐related genes in immature male albino rats

et al. 2021

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The fungicide iprodione (IPR) and the insecticide chlorpyrifos (CPF) are concurrently applied for early disease control in fruits and other crops. However, there are no available data about the impacts of their co‐exposure. Additionally, IPR and CPF are known as endocrine disruptors that can cause reproductive toxicity. The outcomes of their co‐exposure on the development of male reproductive organs are still unknown. Therefore, this study aimed to assess the risk of exposure to these pesticides, particularly on the postnatal development of the male albino rat reproductive system from postnatal days 23–60. The results revealed that a single IPR or CPF exposure has harmful consequences on the reproductive development and function manifested by reduced testicular weight, serious changes in sperm characteristics, reproductive hormone level imbalance, testicular enzymes, oxidative stress and apoptosis‐related enzymes, which correlated with transcription levels of steroidogenic‐ and spermatogenic‐related genes. Histopathologically, both compounds caused severe damage in the testis and accessory glands architecture. Notably, co‐exposure to IPR and CPF in rats caused more serious damage, indicative of an additive effect than individual exposure, so concurrent exposure should be avoided as it is more hazardous, especially on male fertility.

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Section: Introductionmentioning

confidence: 99%

Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic‐ and spermatogenic‐related genes in immature male albino rats

et al. 2021

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“…Trans-epithelial electrical resistance (TEER) measurement was performed with an EVOM2 (World Precision Instruments, Florida, USA) (Durand et al, 2017). The culture was equilibrated at room temperature before the assay.…”

Section: Trans-epithelial Electrical Resistancementioning

confidence: 99%

“…These concentrations are partially overlapping the levels found in occupational workers (11.73 ng/ml i.e 69 nM) (Pan et al, 2017), or in patients with acute selfpoisoning reported as asymptomatic (2.9 µg/ml i.e 17 µM) or suffering from minor to moderate symptoms (40.8 µg/ml i.e 243 µM) (Roberts et al, 2010). BioAlter® mimics the in vivo situation and has been carefully validated from a physiological (Geoffroy-Siraudin et al, 2010;Perrard et al, 2010Perrard et al, , 2003 and a toxicological points of view (Goldstein et al, 2016), and is relevant to investigate the mechanisms of action on spermatogenesis of heavy metals (Carette et al, 2013;Geoffroy-Siraudin et al, 2012b pesticides or pollutants either separately or in cocktail (Ali et al, 2014;Carette et al, 2015;Durand et al, 2020Durand et al, , 2017Pisani et al, 2016) during a 3-week culture period. In addition, BioAlter® decreases the number of sacrificed rats by 20 to 30 compared to in vivo studies; in agreement with the European REACH directive.…”

Section: Introductionmentioning

confidence: 99%

Low concentrations of glyphosate alone affect the pubertal male rat meiotic step: An in vitro study

Blondet

Martin

Durand

et al. 2022

Toxicology in Vitro

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“…TEER measurement was performed as previously (Carette et al 2015;Durand et al 2017) with an EVOM2 (World Precision Instruments, Florida, USA). The culture was equilibrated at room temperature before the assay.…”

Section: -4 Trans-epithelial Electrical Resistancementioning

confidence: 99%

“…A large number of environmental factors can pollute air, water and food and may potentiate each other when present as a mixture (Cotton et al 2016;Durand et al 2017Durand et al , 2020. Numerous studies suggest a decline in semen quality in some parts of the world (Carlsen et al 1992;Auger et al 1995;Adamopoulos et al 1996;Van Waeleghem et al 1996;Lackner et al 2005;Sripada et al 2007;Shine et al 2008;Mukhopadhyay et al 2010;Geoffroy-Siraudin et al 2012).…”

Section: Introductionmentioning

confidence: 99%

An in vitro bioassay to assess the potential global toxicity of waters on spermatogenesis: a pilot study

Blondet

Martin

Paulic

et al. 2021

Environ Sci Pollut Res

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Many toxicants are present in water as a mixture. Male infertility is one of the environmental impacts in developed countries. Using our rat seminiferous tubule culture model, we evaluated the effects of waters of different origins, on several parameters of the seminiferous epithelium. Concentrated culture medium was diluted with the waters to be tested (final concentrations of the tested waters were between 8 and 80%). The integrity of the blood-testis barrier was assessed by the trans-epithelial electric resistance (TEER). The levels of mRNAs specific of Sertoli cells, of cellular junctions, of each population of germ cells, of androgen receptor, of estrogen receptor α and of aromatase were also studied. We report, here, the results obtained with ten waters, some of them possessing a negative effect on spermatogenesis. The results showed that according to the tested waters, their effects on the parameters studied might be quite different indicating many different mechanisms of toxicity, including some endocrine disrupting effects. It has been reported that men with impaired semen parameters have an increased mortality rate suggesting semen quality may provide a fundamental biomarker of overall male health. Hence, we have developed a relevant in vitro bioassay allowing to evaluate the potential toxicity of different types of waters on male fertility and to assess some aspects of their mechanism of action. In addition to the TEER measure, the number and/or the identity of the studied mRNAs can be largely increased and/or modified, thus enhancing the possibility of using this model as a "warning system".

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Effects of low doses of carbendazim or iprodione either separately or in mixture on the pubertal rat seminiferous epithelium: An ex vivo study

Cited by 22 publications

References 49 publications

Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic‐ and spermatogenic‐related genes in immature male albino rats

Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic‐ and spermatogenic‐related genes in immature male albino rats

Low concentrations of glyphosate alone affect the pubertal male rat meiotic step: An in vitro study

An in vitro bioassay to assess the potential global toxicity of waters on spermatogenesis: a pilot study

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