Effects of low ethanol consumption on nonalcoholic steatohepatitis in mice

Takahashi, Yoshihisa; Watabe, Shiori; Togashi-Kumagai, Arisa; Watanabe, Masato; Dungubat, Erdenetsogt; Kusano, Hiroyuki; Kobayashi, Yasuyuki; Harada, Naoki; Yamaji, Ryoichi; Sugimoto, Keiichiro; Fukusato, Toshio

doi:10.1016/j.alcohol.2020.04.004

Cited by 3 publications

(14 citation statements)

References 37 publications

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“…Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (T-Cho), glucose, insulin, and adiponectin levels were measured as described in our previous studies [32,33].…”

Section: Assessment Of Serum Biochemical Parametersmentioning

confidence: 99%

“…The histopathology was evaluated semi-quantitatively using the validated scoring system by Kleiner et al [34]. Detailed scoring criteria for steatosis, lobular and portal inflammation, hepatocellular ballooning, and perisinusoidal fibrosis are described in our previous papers [32,33]. The method of calculation of NAFLD activity score (NAS) and staging of fibrosis are also described in our previous papers [32,33].…”

Section: Histopathological Analysismentioning

confidence: 99%

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Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

et al. 2020

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Several recent experimental studies have investigated the effects of caffeine and chlorogenic acid (CGA), representative ingredients of coffee, on nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH). However, the results are conflicting, and their effects are yet to be clarified. In the present study, we examined the effects of caffeine and CGA on choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD)-fed mice, relatively new model mice of NASH. Seven-week-old male C57BL/6J mice were divided into the following groups: Control diet (control), CDAHFD (CDAHFD), CDAHFD supplemented with 0.05% (w/w) caffeine (caffeine), and CDAHFD supplemented with 0.1% (w/w) CGA (CGA). After seven weeks, the mice were killed and serum biochemical, histopathological, and molecular analyses were performed. Serum alanine aminotransferase (ALT) levels were significantly higher in the caffeine and CGA groups than in the CDAHFD group. On image analysis, the prevalence of Oil red O-positive areas (reflecting steatosis) was significantly higher in the caffeine group than in the CDAHFD group, and that of CD45R-positive areas (reflecting lymphocytic infiltration) in the hepatic lobule was significantly higher in the caffeine and CGA groups than in the CDAHFD group. Hepatic expression of interleukin (IL)-6 mRNA was higher in the caffeine and CGA groups than in the CDAHFD group, and the difference was statistically significant for the caffeine group. In conclusion, in the present study, caffeine and CGA significantly worsened the markers of liver cell injury, inflammation, and/or steatosis in NASH lesions in mice.

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Section: Assessment Of Serum Biochemical Parametersmentioning

confidence: 99%

Section: Histopathological Analysismentioning

confidence: 99%

Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

et al. 2020

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show abstract

“…The histopathology was evaluated semiquantitatively using the validated scoring system by Kleiner et al [34]. Detailed scoring criteria for steatosis, lobular and portal inflammation, hepatocellular ballooning, and perisinusoidal fibrosis are described in our previous papers [32,33]. The method of calculation of NAFLD activity score (NAS) and staging of fibrosis are also described in our previous papers [32,33].…”

Section: Histopathological Analysismentioning

confidence: 99%

“…We measured TG and T-Cho levels in the liver biochemically using frozen tissues. The detailed methods are described in our previous paper [33]. www.videleaf.com…”

Section: Hepatic Levels Of Tg and T-chomentioning

confidence: 99%

“…The expression of CD36, PPARα, microsome triglyceride transfer protein (MTP), carnitine palmitoyltransferase (CPT) 1A, sterol regulatory elementbinding protein (SREBP)-1c, fatty acid synthase (FASN), acetyl-CoA carboxylase (ACC), phosphoenolpyruvate carboxykinase (PEPCK), and pyruvate carboxylase (PC) genes was measured and the TBP gene was used as the internal control. The methods are the same as additional real-time RT-PCR in our previous study [33], and the primers for each gene are shown in Table 1. www.videleaf.com…”

Section: Real-time Reverse Transcription Polymerase Chain Reaction (Rt-pcr) Analysismentioning

confidence: 99%

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Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

Watabe¹,

Togashi-Kumagai²,

Kobayashi³

et al. 2021

Prime Archives in Nutrition

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Age-dependent sex difference of non-alcoholic fatty liver disease in TSOD and db/db mice

Dungubat

Kusano²,

Mori³

et al. 2022

PLoS ONE

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According to previous clinical studies, the prevalence of non-alcoholic fatty liver disease (NAFLD) is higher in men than women only during the reproductive age. Animal models of NAFLD that reflect sex differences in humans have not been established. In this study, we examined sex differences in the hepatic lesions of Tsumura Suzuki obese diabetes (TSOD) and db/db mice, which are representative genetic models of NAFLD. Male and female TSOD and db/db mice were fed with a normal diet and tap water ad libitum. Six male and female mice of each strain were sacrificed at the ages of 3 and 9 months, respectively, and serum biochemical, pathological, and molecular analyses were performed. Serum aspartate aminotransferase (AST) levels were significantly higher in male than female mice of both strains at the age of 3 months; however, at 9 months, significant sex differences were not observed. Similarly, alanine aminotransferase (ALT) levels were significantly higher in male mice than in female TSOD mice at the age of 3 months; however, at 9 months, significant sex differences were not observed. Image analysis of histological slides revealed that the frequency of the steatotic area was significantly higher in male than female db/db mice at the age of 3 months; however, significant sex differences were not observed at 9 months. The frequency of Sirius red-positive fibrotic area was significantly higher in male than female mice in both strains at the age of 3 months; however, significant sex differences were not observed at 9 months. Serum AST and ALT levels and hepatic steatosis and fibrosis in TSOD and db/db mice showed age-dependent sex differences consistent with those observed in human NAFLD. These mice may be suitable for studying sex differences of the disease.

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Effects of low ethanol consumption on nonalcoholic steatohepatitis in mice

Cited by 3 publications

References 37 publications

Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

Age-dependent sex difference of non-alcoholic fatty liver disease in TSOD and db/db mice

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