Effects of low frequency pulsed electrical current on keratinocytes in vitro

Hinsenkamp, Maurice; Jerčinović, Aleksandra; Graef, Chantal De; Wilaert, F.; Heenen, Michel

doi:10.1002/(sici)1521-186x(1997)18:3<250::aid-bem8>3.0.co;2-1

Cited by 30 publications

(28 citation statements)

References 5 publications

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“…To obtain enough total RNA for microarray and real-time reverse transcriptase polymerase chain reaction (real-time rtPCR) experimentation, 12 explants are sampled and pooled for RNA extraction under each sample condition. To make a link with the previous results [Jercinovic et al, 1996;Hinsenkamp et al, 1997] and validate the success of the biological and electrical stimulation protocols, the same procedure was followed including the culture duration of 15 days. Figure 2.…”

Section: Biological Modelmentioning

confidence: 99%

“…Variation of electric current is uniform and 20% of current density goes through the dermis, and 80% through the culture medium. Electrical pattern (wave form, intensity, frequency of stimulation) shown in Figure 4 is the same as in the in vitro study published by Hinsenkamp et al 1997 and the clinical study on chronic wound healing [Jercinovic et al, 1994]. The choice of daily stimulation is based on a review of the literature on the human application of electric and electromagnetic fields for chronic wound treatment [Vodovnik and Karba, 1992].…”

Section: Electrical Stimulation Patternmentioning

confidence: 99%

“…This model of epidermal growth provides a simplified and well-characterized model to study the biological effects of low-frequency electric fields. This model uses a 40 Hz pulsed asymmetric charge-balanced carrier signal modulated by a fundamental frequency of 0.125 Hz and transmitted by two platinum electrodes [Jercinovic et al, 1996;Hinsenkamp et al, 1997]. On the stimulated cultures, the results showed:…”

Section: Introductionmentioning

confidence: 99%

“…Planimetry, histologic examination, and [H3]-thymidine labeling results published by Hinsenkamp et al 1997 suggested that low-frequency pulsed electrical stimulation had influenced differentiation of the epidermal culture because better structured and mature epidermal layers were formed in areas around stimulated explants. This was achieved at the expense of the proliferation of the keratinocytes culture as the number of mitoses decreased in parallel with the growth area.…”

Section: Introductionmentioning

confidence: 99%

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In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells

Collard

Mertens

Hinsenkamp

2010

Bioelectromagnetics

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An acceleration of differentiation, at the expense of proliferation, is observed after exposure of various biological models to low frequency and low amplitude electric and electromagnetic fields. Following these results showing significant modifications, we try to identify the biological mechanism involved at the cell level through microarray screening. For this study, we use epidermis cultures harvested from human abdominoplasty. Two platinum electrodes are used to apply the electric signal. The gene expressions of 38,500 well-characterized human genes are analyzed using Affymetrix® microarray U133 Plus 2.0 chips. The protocol is repeated on three different patients. After three periods of exposure, a total of 24 chips have been processed. After the application of ELF electric fields, the microarray analysis confirms a modification of the gene expression of epidermis cells. Particularly, four up-regulated genes (DKK1, TXNRD1, ATF3, and MME) and one down-regulated gene (MACF1) are involved in the regulation of proliferation and differentiation. Expression of these five genes was also confirmed by real-time rtPCR in all samples used for microarray analysis. These results corroborate an acceleration of cell differentiation at the expense of cell proliferation.

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Section: Biological Modelmentioning

confidence: 99%

Section: Electrical Stimulation Patternmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells

Collard

Mertens

Hinsenkamp

2010

Bioelectromagnetics

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“…These studies provided insights into the cellular and physiological mechanisms whereby ES enhances wound healing. ES has also been reported to attract keratinocytes to wounds (Nishimura et al 1996), and to facilitate the proliferation and migration of keratinocytes during the wound healing process (Hinsenkamp et al 1997). However, recent studies of the application of ES to wound healing focused on normal skin wounds, and as a result, little information is available on the benefits of ES applied to diabetic wounds.…”

Section: Introductionmentioning

confidence: 99%

High-Voltage Pulsed Current Stimulation Enhances Wound Healing in Diabetic Rats by Restoring the Expression of Collagen, <i>α</i>-Smooth Muscle Actin, and TGF-<i>β</i>1

Kim

Cho

Lee

2014

Tohoku J. Exp. Med.

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Impaired wound healing is a common complication of diabetes mellitus and a major morbidity that leads to pain and severely diminished quality of life. Diabetic wounds are commonly associated with defective immune cell responses or abnormality of extracellular matrix. Various types of electrical stimulation interventions have been used to promote tissue healing. However, it is unclear whether high-voltage pulsed current stimulation (HVPCS) enhances diabetic wound healing. In this study, the effects of HVPCS on wound healing were investigated in diabetic rats. Three groups of rats (10 per group) were used: non-diabetic control, diabetic control, and diabetic rats that were administered HVPCS for 40 minutes daily for 1 week. Rats from control groups were administered sham interventions. Dorsal incision wounds were generated in all animals, and wound-healing rate was determined during one-week intervention. After interventions, we measured the relative expression levels of collagen type I (collagen-I), α-smooth muscle actin (α-SMA), and transforming growth factor-β1 (TGF-β1) mRNAs in the wounded skin. Wound closure was delayed in diabetic control rats compared to the non-diabetic control rats, and the diabetic control rats showed the reduced expression levels of collagen-I, α-SMA and TGF-β1 mRNAs. Importantly, compared to diabetic control rats, rats with HVPCS showed accelerated wound closure and healing (p < 0.01) and restored expression levels of collagen-I (p = 0.02), α-SMA (p = 0.04), and TGF-β1 (p = 0.01) mRNAs. In conclusion, HVPCS may be beneficial for enhancing the healing of diabetic wounds by restoring the expression levels of TGF-β1, collagen-I, and α-SMA.

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In vitro biocompatibility study of electrically conductive polypyrrole-coated polyester fabrics

Zhang

Roy²,

Dugré³

et al. 2001

J. Biomed. Mater. Res.

128

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This study investigated the basic biocompatibility aspects of two types of polypyrrole (PPy)-coated polyester fabrics for possible use as vascular prostheses. These PPy-coated fabrics, PPy-Phos and PPy-Plas, were sterilized with ethylene oxide (EO) and the following characterizations were performed: surface morphology by scanning electron microscope, EO residuals analysis by the headspace method, acute systemic toxicity in the mouse model, hemolysis, blood coagulation time, viability and proliferation of endothelial cells measured with the WST-1 method, and activation of polymorphonuclear (PMN) cells indicated by the specific expression of interleukin 8 mRNA measured by reverse transcription polymerase chain reaction. Virgin polyester fabrics, expanded poly(tetrafluoroethylene) (ePTFE), and medical-grade Bionate 80A poly(carbonate urethane) were used as references in the cell culture experiments. The PPy-coated fabrics revealed different surface morphologies by showing more PPy lamina and clusters on the PPy-Plas. Neither of the PPy-coated fabrics had an adverse effect on hemolysis and coagulation time, and they did not cause any acute systemic toxicity. The EO residual level was as low as 5 ppm or less, which is considered quite acceptable. Although exhibiting a relatively low initial cell adhesion at 24 h, the two PPy-coated samples showed no cytotoxicity at 72 and 168 h. Bionate 80A and ePTFE recorded cytotoxicity at 72 and 168 h, respectively. The virgin fabrics also demonstrated a decrease of viable cells at 72 h that was not significant. The activation of PMN cells induced by both PPy-coated fabrics, the ePTFE, and the negative control was significantly lower than that induced by their respective tumor necrosis factor-alpha controls. These results therefore highlighted the potential of PPy-coated fabrics for use as cardiovascular prostheses. It was suggested that cell adhesion moieties should be incorporated into the PPy/fabric composite to increase cell adhesion and subsequent cell proliferation.

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Effects of low frequency pulsed electrical current on keratinocytes in vitro

Cited by 30 publications

References 5 publications

In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells

In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells

High-Voltage Pulsed Current Stimulation Enhances Wound Healing in Diabetic Rats by Restoring the Expression of Collagen, <i>α</i>-Smooth Muscle Actin, and TGF-<i>β</i>1

In vitro biocompatibility study of electrically conductive polypyrrole-coated polyester fabrics

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