Effects of low-intensity and low-frequency ultrasound combined with tobramycin on biofilms of extended-spectrum beta-lactamases (ESBLs) <i>Escherichia coli</i>

Hou, Yuru; Yang, Min; Hexun, Jiang; Li, Dairong; Du, Yuchen

doi:10.1093/femsle/fnz026

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…It was found that the morphology of the biofilms subjected to the treatment was seriously affected, presenting reduced thickness and a loosened structure. As a consequence, the penetration of the antibiotic increased, which led to an increased antibacterial effect (Hou et al, 2019).…”

Section: Photodynamic Therapy and Ultrasoundsmentioning

confidence: 99%

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

et al. 2020

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Bacterial biofilms represent a major concern at a worldwide level due to the high demand for implantable medical devices and the rising numbers of bacterial resistance. The complex structure of the extracellular polymeric substances (EPS) matrix plays a major role in this phenomenon, since it protects bacteria from antibiotics, avoiding drug penetration at bactericidal concentrations. Besides, this structure promotes bacterial cells to adopt a dormant lifestyle, becoming less susceptible to antibacterial agents. Currently, the available treatment for biofilm-related infections consists in the administration of conventional antibiotics at high doses for a long-term period. However, this treatment lacks efficiency against mature biofilms and for implant-associated biofilms it may be necessary to remove the medical device. Thus, biofilm-related infections represent an economical burden for the healthcare systems. New strategies focusing on the matrix are being highlighted as alternative therapies to eradicate biofilms. Here, we outline reported matrix disruptive agents, nanocarriers, and technologies, such as application of magnetic fields, photodynamic therapy, and ultrasounds, that have been under investigation to disrupt the EPS matrix of clinically relevant bacterial biofilms. In an ideal therapy, a synergistic effect between antibiotics and the explored innovated strategies is aimed to completely eradicate biofilms and avoid antimicrobial resistance phenomena.

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Section: Photodynamic Therapy and Ultrasoundsmentioning

confidence: 99%

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

et al. 2020

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“…In our experiments, acoustic cavitation could occur in ultrasound and radial pressure wave treatments, while the applied shockwaves also had sufficient tensile pressure to trigger additional inertial cavitation bubbles [ 31 ]. This is supported by the fact that the application of LFUS to bacterial biofilms has been shown to significantly impact their viability and morphology [ 32 ], associated with non-inertial cavitation associated with trapped microbubbles [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Additional potential thermal effects [ 32 ] are unlikely candidates to affect the viability of the biofilm in our experiments. Thermal heating is most often associated with ultrasound treatments due to high frequencies and long duty cycles.…”

Section: Discussionmentioning

confidence: 99%

“…As the pulse rate is of only a few Hz in ESWT, heating was only a potential concern in our LFUS treatment. Using a limited 50% duty cycle at low intensities resulted in no expectation of local heating [ 32 ]. In addition, there was a significantly reduced thermal power deposition of subsequent acoustical pulses when cavitation-driven bubble fields were encountered [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

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Shockwaves Increase In Vitro Resilience of Rhizopus oryzae Biofilm under Amphotericin B Treatment

Slezak

Anderson

Hillock

et al. 2022

IJMS

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Acoustical biophysical therapies, including ultrasound, radial pressure waves, and shockwaves, have been shown to harbor both a destructive and regenerative potential depending on physical treatment parameters. Despite the clinical relevance of fungal biofilms, little work exits comparing the efficacy of these modalities on the destruction of fungal biofilms. This study evaluates the impact of acoustical low-frequency ultrasound, radial pressure waves, and shockwaves on the viability and proliferation of in vitro Rhizopus oryzae biofilm under Amphotericin B induced apoptosis. In addition, the impact of a fibrin substrate in comparison with a traditional polystyrene well-plate one is explored. We found consistent, mechanically promoted increased Amphotericin B efficacy when treating the biofilm in conjunction with low frequency ultrasound and radial pressure waves. In contrast, shockwave induced effects of mechanotransduction results in a stronger resilience of the biofilm, which was evident by a marked increase in cellular viability, and was not observed in the other types of acoustical pressure waves. Our findings suggest that fungal biofilms not only provide another model for mechanistical investigations of the regenerative properties of shockwave therapies, but warrant future investigations into the clinical viability of the therapy.

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“…The National Institutes of Health (NIH) agency reports that 65% of microbial diseases and more than 80% of chronic infections are associated with bacterial biofilms, including cystic fibrosis, sinusitis, non-healing wounds, and implanted catheters related infection (Wolfmeier et al, 2018;Hou et al, 2019;Hu et al, 2020). Biofilms associated with antibiotic resistance have been reported to exist in MTB, and the extracellular material in these biofilms is mainly composed of polysaccharides, of which cellulose is a key component (Trivedi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Synergistic antibacterial effects of ultrasound combined nanoparticles encapsulated with cellulase and levofloxacin on Bacillus Calmette-Guérin biofilms

Zhang

Yang

et al. 2023

Front. Microbiol.

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Tuberculosis is a chronic infectious disease, the treatment of which is challenging due to the formation of cellulose-containing biofilms by Mycobacterium tuberculosis (MTB). Herein, a composite nanoparticle loaded with cellulase (CL) and levofloxacin (LEV) (CL@LEV-NPs) was fabricated and then combined with ultrasound (US) irradiation to promote chemotherapy and sonodynamic antimicrobial effects on Bacillus Calmette-Guérin bacteria (BCG, a mode of MTB) biofilms. The CL@LEV-NPs containing polylactic acid-glycolic acid (PLGA) as the shell and CL and LEV as the core were encapsulated via double ultrasonic emulsification. The synthesized CL@LEV-NPs were uniformly round with an average diameter of 196.2 ± 2.89 nm, and the zeta potential of −14.96 ± 5.35 mV, displaying high biosafety and sonodynamic properties. Then, BCG biofilms were treated with ultrasound and CL@LEV-NPs separately or synergistically in vivo and in vitro. We found that ultrasound significantly promoted biofilms permeability and activated CL@LEV-NPs to generate large amounts of reactive oxygen species (ROS) in biofilms. The combined treatment of CL@LEV-NPs and US exhibited excellent anti-biofilm effects, as shown by significant reduction of biofilm biomass value and viability, destruction of biofilm architecture in vitro, elimination of biofilms from subcutaneous implant, and remission of local inflammation in vivo. Our study suggested that US combined with composite drug-loaded nanoparticles would be a novel non-invasive, safe, and effective treatment modality for the elimination of biofilm-associated infections caused by MTB.

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Effects of low-intensity and low-frequency ultrasound combined with tobramycin on biofilms of extended-spectrum beta-lactamases (ESBLs) Escherichia coli

Cited by 13 publications

References 37 publications

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

Innovative Strategies Toward the Disassembly of the EPS Matrix in Bacterial Biofilms

Shockwaves Increase In Vitro Resilience of Rhizopus oryzae Biofilm under Amphotericin B Treatment

Synergistic antibacterial effects of ultrasound combined nanoparticles encapsulated with cellulase and levofloxacin on Bacillus Calmette-Guérin biofilms

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