Effects of Low Intensity Continuous Ultrasound (LICU) on Mouse Pancreatic Tumor Explants

Bazou, Despina; Maimon, Nir; Munn, Lance L.; González, Itzı́ar

doi:10.3390/app7121275

Cited by 8 publications

(10 citation statements)

References 45 publications

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“…Several studies have suggested increased tissue repair owing to ultrasound exposure [8,9], which has led to the increased use of ultrasound equipment when treating bone fracture [10]. However, the acoustic setups of these [8][9][10] and other [11][12][13][14] tissue-repair studies have been such that thermal effects could not be ruled out. In fact, heating is the most plausible explanation for the phenomena observed [15].…”

Section: Introductionmentioning

confidence: 99%

On the Behaviour of Living Cells under the Influence of Ultrasound

Rubin

Anderton

Smalberger

et al. 2018

Fluids

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Medical ultrasound technology is available, affordable, and non-invasive. It is used to detect, quantify, and heat tissue structures. This review article gives a concise overview of the types of behaviour that biological cells experience under the influence of ultrasound only, i.e., without the presence of microbubbles. The phenomena are discussed from a physics and engineering perspective. They include proliferation, translation, apoptosis, lysis, transient membrane permeation, and oscillation. The ultimate goal of cellular acoustics is the detection, quantification, manipulation and eradication of individual cells.

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Section: Introductionmentioning

confidence: 99%

On the Behaviour of Living Cells under the Influence of Ultrasound

Rubin

Anderton

Smalberger

et al. 2018

Fluids

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“…62 Subsequent studies performed in vitro and in vivo, have shown that US at specific wave amplitudes and dose conditions induce cell death in different types of tumors, including leukemia, squamous cell carcinoma and sarcoma under certain acoustic conditions. [27][28][29][30][31][32][33][34] After exposure to US, cells show more classic morphological and molecular features of apoptosis, such as condensation of the nucleus, changes in chromatin position and increased expression of membrane phosphatidylserine. Furthermore, there are changes in the expression of apoptotic BCL-2, such as BAX, BAD and Bak, which promotes the activation of the mitochondrial intrinsic apoptotic pathway releasing cytochrome C to the cytosol, resulting in the activation of caspases 3 and 9.…”

Section: Effects Of Ultrasounds On Gene Expression and Apoptosismentioning

confidence: 99%

“… 20 , 22 , 28 Some studies reported mechanical angiogenesis enhancement. 29 , 30 The effects of low-intensity continuous ultrasounds (LICUS) were studied in a recent paper 31 on the inflammatory response of mouse pancreatic tumor explants. The authors found significant upregulation of IFN-γ, IL-1β and TNF-α in the pancreatic tumor explants.…”

Section: Introductionmentioning

confidence: 99%

Ultrasounds in cancer therapy: A summary of their use and unexplored potential

Díaz-Alejo¹,

González²,

Earl³

2022

Oncol Rev

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Ultrasounds (US) are a non-ionizing mechanical wave, with less adverse effects than conventional pharmacological or surgical treatments. Different biological effects are induced in tissues and cells by ultrasound actuation depending on acoustic parameters, such as the wave intensity, frequency and treatment dose. This non-ionizing radiation has considerable applications in biomedicine including surgery, medical imaging, physical therapy and cancer therapy. Depending on the wave intensity, US are applied as High Intensity Ultrasounds (HIUS) and Low Intensity Pulsed Ultrasounds (LIPUS), with different effects on cells and tissues. HIUS produce thermal and mechanical effects, resulting in a large localized temperature increase, leading to tissue ablation and even tumor necrosis. This can be achieved by focusing low intensity waves emitted from different electrically shifted transducers, known as HIFU (High Intensity Focused Ultrasounds). LIPUS have been used extensively as a therapeutic, surgical and diagnostic tool, with diverse biological effects observed in tissues and cultured cells. US represent a non-invasive treatment strategy that can be applied to selected areas of the body, with limited adverse effects. In fact, tumor ablation using HIFU has been used as a curative treatment in patients with an early stage pancreatic tumor and is an effective palliative treatment in patients with advanced stage disease. However, the biological effects, dose standardization, benefit-risk ratio and safety are not fully understood. Thus, it is an emerging field that requires further research in order to reach its full potential.

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“…We are currently developing novel devices to study the biological effects of low intensity ultrasounds in various types of tumor models, including bioprinted 3D tumor & stroma models. Furthermore, we are developing high-resolution ultrasonic methods for a non-invasive early detection of very small physical changes in tissues related with tumor development such as spatial, thermal and elastic properties [22].…”

Section: Crimson Publishersmentioning

confidence: 99%

Ultrasound Technology as a Novel Treatment Strategy in Pancreatic Cancer

González

Fernández

Rodríguez‐Lorenzo

et al. 2019

NACS

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Adenocarcinoma of the pancreas (PDAC) accounts for 2.4% of all cancers diagnosed and is the fourth leading cause of cancer death, with almost equal rates of incidence and mortality [1]. By 2030, pancreatic cancer is projected to be the second leading cause of cancer-related death [2], surpassing breast, prostate and colorectal cancer. The overall survival at 5 years of around 7.2% as the majority of patients present with advanced disease at diagnosis. Patients with localized disease are treated with surgery, with or without neoadjuvant chemotherapy/ radiotherapy, followed by adjuvant chemotherapy. The majority (around 80%) of patients are treated only with chemotherapy as they have an advanced disease. Patients are treated in the first line with gemcitabine-abraxane or Folfirinox and with Naliri plus 5FU in the second line. There have been few clinical advances in PDAC treatment over the last 20 years and chemotherapy is the only treatment option available for the majority of patients. These tumours are also resistant to many targeted therapies such as anti-EGFR therapy like cetuximab [3] due to the presence of a KRAS mutation in the majority of primary tumors. Personalized medicine strategies have not yet been established in pancreatic cancer as in other more common tumour types. Thus, novel anti-tumour strategies are an important clinical need in order to improve survival rates.

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Effects of Low Intensity Continuous Ultrasound (LICU) on Mouse Pancreatic Tumor Explants

Cited by 8 publications

References 45 publications

On the Behaviour of Living Cells under the Influence of Ultrasound

On the Behaviour of Living Cells under the Influence of Ultrasound

Ultrasounds in cancer therapy: A summary of their use and unexplored potential

Ultrasound Technology as a Novel Treatment Strategy in Pancreatic Cancer

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