Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

Pereira, Pâmela Rosa; Paula, Josué Bruginski de; Cielinski, Juliette; Pilonetto, Marcelo; Bahten, Luiz Carlos Von

doi:10.1590/s0100-69912014000100010

Cited by 25 publications

(16 citation statements)

References 21 publications

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“…Furthermore, to support the decontamination of the oral condition locally, accelerate the healing process, and reduce the pain sensation, the laser device (in an infrared wavelength) was first used, associated with methylene blue (Chimiolux, 5%, DMC, São Carlos -SP, Brazil) to constitute the aPDT modality. The parameters used, such as PIT (5 minutes), the use of a phenotiazine dye (methylene blue), the time of irradiation and dose, were in accordance with those of other investigations [11][12][13][14] and are described in Table 1.…”

Section: Discussionmentioning

confidence: 54%

“…After this, the patient was re-called within 48 hours to verify his clinical condition and we proceeded with the application of PBMT with the same parameters as those shown above to 4 distinct points (around the lesions at points equally far from each other) according to the details described in Table 1, as used in some previous studies ( Figure 1B). [11][12][13][14] The irradiation was repeated 4 times with an interval of 48 hours; therefore, 4 PBMT sessions were performed with the aim of relieving pain (analgesic effect), controlling inflammation, and accelerating the healing process with the main focus being on patient comfort.…”

Section: Case Reportmentioning

confidence: 99%

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The Combination of Antimicrobial Photodynamic Therapy and Photobiomodulation Therapy for the Treatment of Palatal Ulcers: A Case Report

Maya¹,

Ladeira

Maya

et al. 2020

J Lasers Med Sci

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Introduction: One of the unexpected side effects of the Haas type palatal expander is ulcers progressing to necrotic lesions in the palatal area due to poor hygiene. The use of antibiotic therapy is mandatory. However, long periods of healing/pain and the need for a systemic host response with the aid of metabolization, especially in children, are issues that should be taken into account in the management of this type of injury. Since phototherapy modalities (antimicrobial photodynamic therapy [aPDT] and photobiomodulation therapy [PBMT]) are able to enhance and accelerate the healing process and reduce the bacterial load, this case report aimed to describe the use of the above-mentioned therapies to treat palatal ulcers occurring during orthodontic expansion. Case Report: The patient, a 10-year-old boy, with a chief complaint of bleeding and continuous pain in the region of his expander was verified on a follow-up visit. After a dental examination, the expander was removed and two necrotic lesions which were in contact with the acrylic part of the tooth-tissue expander were found in the palatal region. The proposal was to use one aPDT session with methylene blue followed by 4 sessions of PBMT with a red laser diode. On the 5th day, reorganized tissue was verified, with the absence of bleeding, swelling, and pain. On the 20th day of follow-up, the area showed no signs of inflammation, healthy tissue without any pathological clinical symptoms, and complete wound healing. Conclusion: The concomitant use of PBMT and aPDT therapies may be considered feasible as an adjunct treatment to manage palatal ulcers resulting from the incorrect use of tooth-tissue types of expanders

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Section: Discussionmentioning

confidence: 54%

Section: Case Reportmentioning

confidence: 99%

The Combination of Antimicrobial Photodynamic Therapy and Photobiomodulation Therapy for the Treatment of Palatal Ulcers: A Case Report

Maya¹,

Ladeira

Maya

et al. 2020

J Lasers Med Sci

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“…Another study that used 904 nm wavelength laser and a fluence of 3 J/cm 2 for seven days on Wistar rats did not alter the growth of S. aureus in vitro [20]. One of the postulated reasons could be that the fluence used for antibacterial effects in the study was too small to generate a noticeable effect.…”

Section: Discussionmentioning

confidence: 99%

“…Another postulated factor may be single wavelength parameters and the difference in cell structure and virulence factor [20]. Usually, the bactericidal effect of LLLT is due to the direct effect on the bacterial membrane due to absorption of photons by endogenous chromophores, with simultaneous production of highly reactive and cytotoxic molecules which causes disruption of the membrane and resulting in bacterial killing.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model

et al. 2019

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Background and Objectives: The aim of this study was to compare the effects of low-level laser therapy and continuous microwave diathermy on the growth of Gram-negative and Gram-positive bacteria and to establish their efficacy as an alternative therapeutic modality. Materials and methods: Laser fluence of 13 Joules (J)/cm2, 18 J/cm2 and 30 J/cm2 were used against several bacterial strains. Microwave dosages of 25, 50 and 100 watts (W) were used, respectively. Results: A significant difference between the three groups was observed using repeated analysis of variance (RANOVA) (F value: 0.74, and p value: 0.001). The Greenhouse–Geisser correction (GG) revealed significant results for laser irradiation alone. However, effect size calculation showed effects with microwave diathermy as well as laser fluence. Conclusions: Low-level laser therapy appears to be an effective modality of treatment when compared with continuous microwave diathermy on the Gram-negative and the Gram-positive bacterial strains tested. Microwave diathermy revealed large and medium effects on the bacterial cell counts with dominant effects on Gram-negative strains.

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“…Materials that showed turbidity after incubation for 24 or 48 hours were seeded and stored in MacConkey and Mannitol Agar. The developed colonies were submitted to morphological and tannic analyzes through Rugai Tubes with Lysine and Gram staining, in which it was possible to identify the pseudomonas aeruginosa as the bacterium isolated from the pressure ulcer [14,15].…”

Section: Collection Of Biological Materials and Microbiological Analysismentioning

confidence: 99%

Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

Mm¹

2018

VIJ

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Pressure ulcers are injuries that can cause bacterial proliferation, and when infected are associated with increased morbidity and mortality. Thus, photodynamic therapy becomes an adjunct to the treatment with medications because it has the bactericidal capacity. In this way, this study aims to analyze in vitro the bactericidal effect of methylene blue by associating it with the low-level laser in the wavelength of 830nm to determine which therapeutic doses present better efficacy in the treatment of bacteria pseudomonas aeruginosa. The microbiological material was collected by an aseptic swab and the antimicrobial activity was verified by disc diffusion method using sterile filter paper. Methylene blue was used at concentrations of 0,001% and 0,005% and the low-level laser of 830nm at the intensities of 4J/cm², 8J/cm², 10J/cm² and 14J/cm², being tested in three plates for each dose, as well as the low-level laser and the methylene blue, were tested separately. The results were statistically analyzed by SPSS through the "t-test for dependent samples". It was verified that there was reduction of the bacterial activity with the application of the low-level laser in doses of 10J/cm² (p=0,00) and 14J/cm² (p=0,03) added to them ethylene blue in 0.005% concentration, however, there was a greater reduction of bacterial proliferation in the application of 10J/cm². Thus, it was concluded that the photodynamic therapy applied in the concentration of 0.005% of the methylene blue combined with the low-level laser of 830nm of 10J/cm² and 14J/cm² of intensity, present a reduction in the amount of bacterial proliferation.Once the bacteria multiply in the pressure ulcer, new clinical signs and symptoms are observed, worsening the Research ArticleVolume 2 Issue 1

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Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds

Cited by 25 publications

References 21 publications

The Combination of Antimicrobial Photodynamic Therapy and Photobiomodulation Therapy for the Treatment of Palatal Ulcers: A Case Report

The Combination of Antimicrobial Photodynamic Therapy and Photobiomodulation Therapy for the Treatment of Palatal Ulcers: A Case Report

Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model

Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

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