Effects of low intensity vibration on bone and muscle in rats with spinal cord injury

Bramlett, Helen M.; Dietrich, W. Dalton; Marcillo, Alexander; Mawhinney, Lana J.; Furones-Alonso, Ofelia; Bregy, Amadé; Peng, Yangyang; Wu, Yong; Pan, Jiangping; Wang, J.; Guo, X. Edward; Bauman, William A.; Cardozo, Christopher P.; Qin, Weiping

doi:10.1007/s00198-014-2748-8

Cited by 38 publications

(50 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 In an SCI animal model, however, the effects of WBV on bone remain unclear. Bramlett et al 26 reported that low-intensity vibration (LIV), which started on the 28th day after SCI and continued for 35 days, improved selected bone markers, but not BMD of the femur and tibia, in 3-month-old SCI rats. Bone damages were found within 3 weeks after SCI in 6-week-old rats 27 and were more severe in SCI rats than in ovariectomized or sciatic neurectomy rats at the age of 6 weeks.…”

Section: Osteoporosis Is a Well-known Complication Of Spinal Cord Injmentioning

confidence: 99%

“…Bramlett et al 26 showed that LIV for 35 days does not alter BMD and trabecular architecture in the contusion SCI model rats aged 3 months. In chronic SCI patients, either, LIV was not effective in improving BMD and TBMS.…”

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

“…In line with this idea, the present study shows that WBV, initiated on the 8th day after SCI, is effective in attenuating the deterioration in bone mass, TBMS and muscle weight. Bramlett et al 26 , who started LIV on the 28th day after SCI, suggested Table 3 Results of serum biochemical analyses that LIV, initiated earlier after SCI and/or continued for a longer period, may improve bone mass. In the present study as well as in previous studies, [28][29][30]34 dry bone weight, ash weight and BV/TV decreased, and TBMS parameters changed during the early stage of SCI in 6-8-week-old rats.…”

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

“…35 Thus, rapid bone loss after SCI appears to be caused by the consistent increase in bone resorption. Bramlett et al 26 reported that serum levels of C-terminal telopeptide of type I collagen degradation products (CTX) increased significantly in the SCI and SCI+LIV groups compared with the SHAM group. On the other hand, serum OC levels were significantly higher in the SCI+LIV and SHAM groups than in the SCI group, 26 suggesting that LIV can promote bone formation even after SCI.…”

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

“…Bramlett et al 26 reported that serum levels of C-terminal telopeptide of type I collagen degradation products (CTX) increased significantly in the SCI and SCI+LIV groups compared with the SHAM group. On the other hand, serum OC levels were significantly higher in the SCI+LIV and SHAM groups than in the SCI group, 26 suggesting that LIV can promote bone formation even after SCI. In the present study, although serum OC levels in the SCI and SCI+WBV groups decreased after 1 week of WBV treatment compared with the SHAM group, the OC levels of the SCI+WBV group reached the OC levels of the SHAM group after 2 weeks of WBV treatment.…”

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

See 4 more Smart Citations

Whole-body vibration can attenuate the deterioration of bone mass and trabecular bone microstructure in rats with spinal cord injury

et al. 2015

View full text Add to dashboard Cite

Study design: Experimental, controlled study. Objective: To examine the effects of whole-body vibration (WBV) on bone mass and trabecular bone microstructure (TBMS) during the early stage in juvenile rats with spinal cord injury (SCI). Setting: Studied at the Kio University in Japan. Methods: Thirty-four 8-week-old male Wistar rats were divided into 3 groups: the SCI group, the sham-operation group (SHAM) and the SCI+WBV group. WBV started on the 8th day after SCI. After 1 or 2 weeks of WBV treatment, measurements of tissue mineral density, trabecular bone mineral content (BMC) and parameters of TBMS were obtained by scanning the proximal tibias with x-ray micro-computed tomography. Serum levels of osteocalcin (OC) and of tartrate-resistant acid phosphatase 5b (TRACP 5b) were measured with ELISA. Results: BMC, volume bone mineral density, bone volume (BV), BV fraction (BV/tissue volume) and connectivity density (Conn.D) of TBMS parameters were significantly higher in SCI+WBV rats than in SCI rats after 2-week WBV. The BMC and BV/TV of bone mass index correlated well with Conn.D, suggesting the preservation of Conn.D. induced by WBV. SCI+WBV rats showed a decrease in serum OC after 1-week WBV, but a quick recovery from that after 2-week WBV. There was no difference in serum TRACP 5b among the 3 groups throughout the experimental period. Conclusion: WBV treatment could attenuate the bone deterioration that occurs during the early stage in juvenile rats with SCI. In a clinic, this early WBV intervention may be an effective rehabilitation modality for preventing bone fragility in SCI patients. Spinal Cord (2016) 54, 597-603; doi:10.1038/sc.2015.220; published online 22 December 2015 INTRODUCTION Osteoporosis is a well-known complication of spinal cord injury (SCI).The mechanism of bone loss by SCI is complex and is constituted by multiple factors involving mechanical, neurovascular, hormonal and metabolic factors. 1,2 Bone loss following SCI begins within the first 6 months after the injury, and stabilizes between 12 and 16 months. 3 Bone loss is especially prominent in sublesional bones such as the distal femur and proximal tibia. [4][5][6][7][8] In fact, bone mass in the distal femur and proximal tibia decreased by 51% and 70%, respectively, within 1 year after the injury in SCI patients. 6 These two bones were so fragile that even a weak physical impact could cause a fracture in SCI patients. 7,8 Therefore, the frequency of fracture in SCI patients was about twice as high as that of healthy people. 9 Edwards et al. 10 showed that in the femur and tibia of acute SCI patients trabecular bone mineral content (BMC) was reduced by 3.1-4.4% per month and volumetric bone mineral density (BMD) by 2.7-4.7% per month within 4 months after the injury. In addition, cortical BMC and volumetric BMD were noticeably reduced, and furthermore bone strength indices were decreased. 10 The incidence of SCI is higher in young people aged 15-24 years. 11 Children (aged 5-13 years) with

show abstract

Section: Osteoporosis Is a Well-known Complication Of Spinal Cord Injmentioning

confidence: 99%

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

Section: Effects Of Vibration Stimulus On Bone In Sci Ratsmentioning

confidence: 99%

See 3 more Smart Citations

Whole-body vibration can attenuate the deterioration of bone mass and trabecular bone microstructure in rats with spinal cord injury

et al. 2015

View full text Add to dashboard Cite

show abstract

Anti‐Siglec‐15 Antibody Prevents Marked Bone Loss after Acute Spinal Cord Injury‐Induced Immobilization in Rats

Peng,

Langermann,

Kothari

et al. 2023

JBMR Plus

View full text Add to dashboard Cite

Rapid and extensive sublesional bone loss after spinal cord injury (SCI) is a difficult medical problem that has been refractory to available interventions except the antiresorptive agent denosumab (DMAB). While DMAB has shown some efficacy in inhibiting bone loss, its concurrent inhibition of bone formation limits its use. Sialic acid‐binding immunoglobulin‐like lectin (Siglec)‐15 is expressed on the cell surface of mature osteoclasts. Anti‐Siglec‐15 antibody (Ab) has been shown to inhibit osteoclast maturation and bone resorption while maintaining osteoblast activity, which is distinct from current antiresorptive agents that inhibit the activity of both osteoclasts and osteoblasts. The goal of the present study is to test a Siglec‐15 Ab (NP159) as a new treatment option to prevent bone loss in an acute SCI model. To this end, 4‐month‐old male Wistar rats underwent complete spinal cord transection and were treated with either vehicle or NP159 at 20 mg/kg once every 2 weeks for 8 weeks. SCI results in significant decreases in bone mineral density (BMD, −18.7%), trabecular bone volume (−43.1%), trabecular connectivity (−59.7%), and bone stiffness (−76.3%) at the distal femur. Treatment with NP159 almost completely prevents the aforementioned deterioration of bone after SCI. Blood and histomorphometric analyses revealed that NP159 is able to greatly inhibit bone resorption while maintaining bone formation after acute SCI. In ex vivo cultures of bone marrow cells, NP159 reduces osteoclastogenesis while increasing osteoblastogenesis. In summary, treatment with NP159 almost fully prevents sublesional loss of BMD and metaphysis trabecular bone volume and preserves bone strength in a rat model of acute SCI. Because of its unique ability to reduce osteoclastogenesis and bone resorption while promoting osteoblastogenesis to maintain bone formation, Siglec‐15 Ab may hold greater promise as a therapeutic agent, compared with the exclusively antiresorptive or anabolic agents that are currently used, in mitigating the striking bone loss that occurs after SCI or other conditions associated with severe immobilization. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.

show abstract

Sclerostin Antibody Preserves the Morphology and Structure of Osteocytes and Blocks the Severe Skeletal Deterioration After Motor-Complete Spinal Cord Injury in Rats

Qin

Peng

et al. 2015

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Unloading, neural lesions, and hormonal disorders after acute motor-complete spinal cord injury (SCI) cause one of the most severe forms of bone loss, a condition that has been refractory to available interventions tested to date. Thus, these features related to acute SCI provide a unique opportunity to study complex bone problems, potential efficacious interventions, and mechanisms of action that are associated with these dramatic pathological changes. This study was designed to explore the therapeutic potential of sclerostin antibody (Scl-Ab) in a rat model of bone loss after motor-complete SCI, and to investigate mechanisms underlying bone loss and Scl-Ab action. SCI rats were administered Scl-Ab (25 mg/kg/week) or vehicle beginning 7 days after injury then weekly for 7 weeks. SCI resulted in significant decreases in bone mineral density (-25%) and trabecular bone volume (-67%) at the distal femur; Scl-Ab completely prevented these deteriorations of bone in SCI rats, concurrent with markedly increased bone formation. Scanning electron microscopy revealed that SCI reduced numbers of osteocytes and dendrites concomitant with a morphology change from a spindle to round shape; Scl-Ab corrected these abnormalities in osteocytes. In ex vivo cultures of bone marrow cells, Scl-Ab inhibited osteoclastogenesis, and promoted osteoblastogenesis accompanied by increases in mRNA levels of LRP5, osteoprotegerin (OPG), and the OPG/RANKL ratio, and a decrease in DKK1 mRNA. Our findings provide the first evidence that robust bone loss after acute motor-complete SCI can be blocked by Scl-Ab, at least in part, through the preservation of osteocyte morphology and structure and related bone remodeling. Our findings support the inhibition of sclerostin as a promising approach to mitigate the striking bone loss that ensues after acute motor-complete SCI, and perhaps other conditions associated with disuse osteoporosis as a consequence of neurological disorders.

show abstract

Effects of low intensity vibration on bone and muscle in rats with spinal cord injury

Abstract: The findings demonstrate an ability of LIV to improve selected biomarkers of bone turnover and gene expression and to reduce osteoclastogenesis. The study indicates a possibility that LIV initiated earlier after SCI and/or continued for a longer duration would increase bone mass.

Cited by 38 publications

References 56 publications

Whole-body vibration can attenuate the deterioration of bone mass and trabecular bone microstructure in rats with spinal cord injury

Whole-body vibration can attenuate the deterioration of bone mass and trabecular bone microstructure in rats with spinal cord injury

Anti‐Siglec‐15 Antibody Prevents Marked Bone Loss after Acute Spinal Cord Injury‐Induced Immobilization in Rats

Sclerostin Antibody Preserves the Morphology and Structure of Osteocytes and Blocks the Severe Skeletal Deterioration After Motor-Complete Spinal Cord Injury in Rats

Contact Info

Product

Resources

About