Effects of low-magnitude high-frequency vibration on osteoblasts are dependent on estrogen receptor α signaling and cytoskeletal remodeling

Haffner‐Luntzer, Melanie; Lackner, Ina; Liedert, Astrid; Fischer, Verena; Ignatius, Anita

doi:10.1016/j.bbrc.2018.08.023

Cited by 24 publications

(25 citation statements)

References 34 publications

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“…Transcription activation is also variably dependent on the amplitude, frequency, and duration of mechanical stimuli [52,54]. Moreover, the sensitivity to mechanical signals in bone is of course strongly modulated by other signaling molecules, as it was for example shown for estrogens and the mechano-related effects on fracture healing [15,17]. In addition, the relevance of pathways such as Hippo and YAP/TAZ signaling as well as Notch signaling in skeletal precursors and in endothelial cells has only recently been established in muscle and bone mechanotransduction [55][56][57][58].…”

Section: Mechanosensing and Mechanotransductionmentioning

confidence: 99%

“…Nevertheless, much more research is needed to further explore the beneficial versus harmful effects in health and disease. Above all, this applies to conditions where exercise-and mechanoresistance or the dysregulation of mechanoresponse are part of the disease or of aging-related changes [12,[15][16][17]. For the time being, it is probably correct to state that vibration techniques if correctly applied may help to delay disuse-related and microgravity-related muscle and bone loss, but the specific molecular mechanisms of exercise resistance in disease have still to be unraveled to efficiently fight the respective aging-associated diseases.…”

Section: Forces Generated By Exercise Locomotion and External Vibramentioning

confidence: 99%

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Interactions between Muscle and Bone—Where Physics Meets Biology

et al. 2020

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Muscle and bone interact via physical forces and secreted osteokines and myokines. Physical forces are generated through gravity, locomotion, exercise, and external devices. Cells sense mechanical strain via adhesion molecules and translate it into biochemical responses, modulating the basic mechanisms of cellular biology such as lineage commitment, tissue formation, and maturation. This may result in the initiation of bone formation, muscle hypertrophy, and the enhanced production of extracellular matrix constituents, adhesion molecules, and cytoskeletal elements. Bone and muscle mass, resistance to strain, and the stiffness of matrix, cells, and tissues are enhanced, influencing fracture resistance and muscle power. This propagates a dynamic and continuous reciprocity of physicochemical interaction. Secreted growth and differentiation factors are important effectors of mutual interaction. The acute effects of exercise induce the secretion of exosomes with cargo molecules that are capable of mediating the endocrine effects between muscle, bone, and the organism. Long-term changes induce adaptations of the respective tissue secretome that maintain adequate homeostatic conditions. Lessons from unloading, microgravity, and disuse teach us that gratuitous tissue is removed or reorganized while immobility and inflammation trigger muscle and bone marrow fatty infiltration and propagate degenerative diseases such as sarcopenia and osteoporosis. Ongoing research will certainly find new therapeutic targets for prevention and treatment.

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Section: Mechanosensing and Mechanotransductionmentioning

confidence: 99%

Section: Forces Generated By Exercise Locomotion and External Vibramentioning

confidence: 99%

Interactions between Muscle and Bone—Where Physics Meets Biology

et al. 2020

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“…First, the inconsistency of what is considered “high frequency” and what is considered “low frequency”. For example, some studies describe vibrations of 30–100 Hz as high frequency [ 206 , 208 , 220 , 222 ] (the perspective of a whole body platform that moves mechanically), whereas other studies report anywhere from 0 to 200 Hz as low frequency [ 70 , 115 , 119 , 155 , 180 ] (the perspective of sound). This is largely due to the scientific contexts in which researchers are interested, but such contextual labels make the synthesis of knowledge in vibration more difficult than it needs to be.…”

Section: Discussionmentioning

confidence: 99%

“…Estrogen levels may also influence whether vibration generates positive or null effects on bone cells. Haffner-Luntzer et al [ 222 ] found that vibration applied at 45 Hz stimulation of osteoblast cells had either positive bone forming effects of gene expression or a null effect depending on the presence of estrogen. They found that bone cells increased COX-2 gene expression, cell metabolic activity, and cell proliferation after vibration in the absence of estrogen, whereas the contrary was true in the presence of estrogen [ 222 ].…”

Section: Musculoskeletal Effectsmentioning

confidence: 99%

Possible Mechanisms for the Effects of Sound Vibration on Human Health

Bartel

Mosabbir

2021

Healthcare

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This paper presents a narrative review of research literature to “map the landscape” of the mechanisms of the effect of sound vibration on humans including the physiological, neurological, and biochemical. It begins by narrowing music to sound and sound to vibration. The focus is on low frequency sound (up to 250 Hz) including infrasound (1–16 Hz). Types of application are described and include whole body vibration, vibroacoustics, and focal applications of vibration. Literature on mechanisms of response to vibration is categorized into hemodynamic, neurological, and musculoskeletal. Basic mechanisms of hemodynamic effects including stimulation of endothelial cells and vibropercussion; of neurological effects including protein kinases activation, nerve stimulation with a specific look at vibratory analgesia, and oscillatory coherence; of musculoskeletal effects including muscle stretch reflex, bone cell progenitor fate, vibration effects on bone ossification and resorption, and anabolic effects on spine and intervertebral discs. In every category research on clinical applications are described. The conclusion points to the complexity of the field of vibrational medicine and calls for specific comparative research on type of vibration delivery, amount of body or surface being stimulated, effect of specific frequencies and intensities to specific mechanisms, and to greater interdisciplinary cooperation and focus.

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“…Considering that external biophysical stimulation has been implicated in regulating actin cytoskeletal remodeling in MSCs, this was also examined in osteoblasts by Haffner-Luntzer et al (2018b), showing that actin remodeling is increased by LMHFV treatment. In addition, Gao et al demonstrated an increased number of microfilaments and thicker stress fibers in the vibrated group, suggesting that the vibration-induced effects on osteoblasts might be dependent on cytoskeletal rearrangement.…”

Section: Osteoblasts and Lmhfvmentioning

confidence: 99%

Influence of Low-Magnitude High-Frequency Vibration on Bone Cells and Bone Regeneration

Steppe

Liedert

Ignatius

et al. 2020

Front. Bioeng. Biotechnol.

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Bone is a mechanosensitive tissue for which mechanical stimuli are crucial in maintaining its structure and function. Bone cells react to their biomechanical environment by activating molecular signaling pathways, which regulate their proliferation, differentiation, and matrix production. Bone implants influence the mechanical conditions in the adjacent bone tissue. Optimizing their mechanical properties can support bone regeneration. Furthermore, external biomechanical stimulation can be applied to improve implant osseointegration and accelerate bone regeneration. One promising anabolic therapy is vertical whole-body low-magnitude high-frequency vibration (LMHFV). This form of vibration is currently extensively investigated to serve as an easyto-apply, cost-effective, and efficient treatment for bone disorders and regeneration. This review aims to provide an overview of LMHFV effects on bone cells in vitro and on implant integration and bone fracture healing in vivo. In particular, we review the current knowledge on cellular signaling pathways which are influenced by LMHFV within bone tissue. Most of the in vitro experiments showed that LMHFV is able to enhance mesenchymal stem cell (MSC) and osteoblast proliferation. Furthermore, osteogenic differentiation of MSCs and osteoblasts was shown to be accelerated by LMHFV, whereas osteoclastogenic differentiation was inhibited. Furthermore, LMHFV increased bone regeneration during osteoporotic fracture healing and osseointegration of orthopedic implants. Important mechanosensitive pathways mediating the effects of LMHFV might be the Wnt/beta-catenin signaling pathway, the estrogen receptor (ER) signaling pathway, and cytoskeletal remodeling.

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Effects of low-magnitude high-frequency vibration on osteoblasts are dependent on estrogen receptor α signaling and cytoskeletal remodeling

Cited by 24 publications

References 34 publications

Interactions between Muscle and Bone—Where Physics Meets Biology

Interactions between Muscle and Bone—Where Physics Meets Biology

Possible Mechanisms for the Effects of Sound Vibration on Human Health

Influence of Low-Magnitude High-Frequency Vibration on Bone Cells and Bone Regeneration

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