Effects of low temperature, genotype and culture media on in vitro androgenic answer of pepper (Capsicum annuum L.)

Popova, Teodora P.; Grozeva, Stanislava; Todorova, Velichka; Stankova, Gergana; Anachkov, Nikolay; Родева, Величка

doi:10.1007/s11738-016-2294-4

Cited by 26 publications

(18 citation statements)

References 34 publications

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“…One-day pretreatment of buds with 4°C was ideal and it emphatically influenced embryogenic process (Ari et al, 2016a;Supena and Custers, 2011). A study by Popova et al (2016) stated that when flower bud is pretreated with low temperature in C. annuum genotypes a reduction in embryogenic response is seen. Whereas when a cold pretreatment of 48 h is given slight increase in direct embryo formation was reported.…”

Section: Pretreatment Of Anthermentioning

confidence: 99%

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Jha¹,

Choudhary²,

Agarwal³

2021

PLANT ARCHIVES

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Capsicum spp. is a horticulture crop with great potential and is supposed to be the fourth most significant vegetable in the earth. Capsicum genus covers several dozens of species which contain vast range of nutritional and medicinal properties. The process of inbreed development for hybrid variety production is fastened by the use of doubled haploid (DH) system. Doubled haploid (DH) system plays an important role in breeding programs and developmental studies. The production of haploid and doubled haploid plants using the anther cultures of the desired genotype is one of opportunities for rapid stabilization of new variants. There is an ongoing research to develop and improve the effectiveness of these methods by looking at the factors involved in different phases like induction and regeneration. Factors predominantly incorporate condition of donor plants, plant genotypes, medium components, cultured gametophytic cells temperature treatments of (cold pre-treatment, heat shock), the developmental period of early on gametophytic cells and use of various segments like Charcoal and plant development regulators. Stress treatment is one of the conditions which are utilized for the incitement of gametophytic cells to modify toward the sporophytic pathway.

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Section: Pretreatment Of Anthermentioning

confidence: 99%

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Jha¹,

Choudhary²,

Agarwal³

2021

PLANT ARCHIVES

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“…23 . Cold pretreatment also has a positive effect on direct embryo induction in pepper (Capsicum annuum L.) 24 . In our study, 4 °C cold pretreatment of flower buds for 7 days had the strongest effect on unpollinated ovule culture in vitro and led to the greatest increase in adventitious bud induction.…”

Section: Discussionmentioning

confidence: 99%

Haploid induction via unpollinated ovule culture in Gerbera hybrida

Fan

Cheng

Zhao

et al. 2020

Sci Rep

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Ovule-derived haploid culture is an effective and important method for genetic study and plant breeding. Gerbera hybrida is a highly heterozygous species, and the lack of homozygous lines presents a challenge for molecular genetic research. Therefore, we performed haploid induction through unpollinated ovule culture and evaluated the effects of several important factors on this culturing procedure in G. hybrida, including genotype, low temperature, and the development seasons of the ovules. Among 45 G. hybrida cultivars analyzed, 29 cultivars exhibited adventitious bud induction via in vitro unpollinated ovule culture with significant different responses, indicating that the genotype of donor plants was a vital factor for inducibility. Four cultivars with significantly different induction rates, including one non-induced cultivar, were selected to analyze seasonal effects. Ovules extracted in the summer consistently had the highest induction rates, and even the non-induced cultivar included in the analysis could be induced at low levels when ovules from summer were used. Low temperature treatment could also promote adventitious bud induction, and in particular, a strong and significant effect was detected after 7 days of cold treatment. Ploidy level measurements by flow cytometry revealed that 288 ovule-derived regenerants were haploid (55.17%) and 218 lines were diploid (41.76%). Moreover, genetic stability analysis of the regenerants indicated 100% similarity to the marker profile of the mother plant. This is the first report of ovule-derived haploids in G. hybrida, which may facilitate the development of homozygous lines for molecular research and plant breeding. Gerbera hybrida is one of the most popular ornamental plants worldwide 1. Since most of the commecial cultivars were bred from the crossing of wild-type G. jamesonii and G. viridifolia, the genome of G. hybrida is highly heterozygous 2. Hundreds of G. hybrida varieties with extremely rich flower color patterns are available in the flower market, including white, yellow, red, pink, purple, and brown, and G. hybrida ranks fourth in cut flowers after rose, chrysanthemum, and tulip 3. As a highly heterozygous species, G. hybrida naturally harbors genetic diversity, which is beneficial for hybridization breeding. However, the lack of a homozygous genome is challenging for molecular genetic research, including forward genetic screening and mutation mapping. Ploidy breeding, the modification of the number of chromosome sets in a plant genome, is frequently used in ornamental plant breeding to induce novel variation and to create homogenous lines 4. A commonly used protocol for ploidy breeding is haploid induction, which refers to haploid regeneration via a single gamete cell under specific conditions, using either pollen (anther or microspore culture) or egg cells (ovule culture) 5. Essentially, the applicability of haploid induction is the main trigger for ornamental breeding, and some haploids of ornamentals have been commercially developed, including the ...

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“…Ercan and Ayar Şensoy (2011) studied the androgenic response in pepper cultivars after a pre-treatment with cold (4 °C) and darkness for 24 hours and obtained 44 embryos from 2398 anthers in in vitro culture. Later, Popova et al (2016) assessed the embryogenic response of C. annuum L. to different lengths of a pre-treatment of low temperature and darkness (during the first eight days). The results indicated that embryogenic efficiency decreases at low temperatures and under photoperiod inhibition.…”

Section: Composition Of Culture Media and Culture Conditionsmentioning

confidence: 99%

Androgenic studies in the production of haploids and doubled haploids in Capsicum spp.

Sánchez¹,

Coronado

2020

Rev. Fac. Nac. Agron. Medellín

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Capsicum spp. is a horticultural crop of agronomic interest and is considered the fourth most important vegetable in the world. It is an important nutritional and medicinal source, and its production generates employment in the tropics. In this species, the genetic variability is wide and with great potential, which has been exploited to generate outstanding varieties. Breeding programs seek different alternatives to accelerate the production of improved varieties with desirable agronomic characteristics. These objectives can be achieved with the production of haploid and double haploid plants via androgenesis or gynogenesis, being androgenesis the approach most used for paprika cultures. The purpose of this review is to present the results of different researches in obtaining haploids and doubled haploids in cultivars of Capsicum spp. and its impact on the genetic improvement of this crop.

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Effects of low temperature, genotype and culture media on in vitro androgenic answer of pepper (Capsicum annuum L.)

Cited by 26 publications

References 34 publications

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Haploid induction via unpollinated ovule culture in Gerbera hybrida

Androgenic studies in the production of haploids and doubled haploids in Capsicum spp.

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