Effects of Low Temperatures on Transmembrane Potentials of Single Fibers of the Rabbit Atrium

Marshall, J. F.

doi:10.1161/01.res.5.6.664

Cited by 73 publications

(28 citation statements)

References 7 publications

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“…4 that the slowing of the rate of diastolic depolarization by lowering the temperature leads to a decrease in the frequency of spontaneous action potentials in the early embryonic precontractile heart. This behavior is similar to that in the adult rabbit Purkinje fiber noted by CoRABOEUF and WEIDMANN (1954) and in the rabbit atria (MARSHALL, 1957). Thus, this suggests that temperaturesensitive structures which would essentially determine the rate of diastolic deporalization have already been formed during the early phases of cardiogenesis.…”

Section: Discussionsupporting

confidence: 87%

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Temperature dependence of spontaneous electrical activity in early embryonic heart monitored optically with a potential-sensitive dye.

et al. 1983

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The effects of temperature on spontaneous action potential activity in the 7-9 somite embryonic precontractile chick hearts were investigated using an optical method for monitoring membrane potential. A potential-sensitive merocyanine-rhodanine dye was used as an optical probe.Cooling caused a marked decrease in the frequency of occurrence of the spontaneous action potential.This slowing was associated with a decreased rate of diastolic depolarization of the pacemaker action potential.When the temperature was lowered, a decrease in the rate of rise and a prolongation of duration of the optical action signal also occurred.In addition, cooling resulted in a disordering of the rhythmicity of the action potential, and a decrease in the conduction velocity of excitation.Ql0 values for such parameters were calculated : there were slight decreases in the Q1° values for the frequency and conduction velocity for 7-9 somite embryonic hearts.

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Section: Discussionsupporting

confidence: 87%

“…Pacemaker potential In adult hearts, the rate of depolarization during the pacemaker potential is highly temperature-dependent (CORABOEUF and WEIDMANN, 1954;MARSHALL, 1957;YAMAGISHI and SANG, 1967). Accordingly, it would be expected that the pacemaker potentials depend also on temperature in the early embryonic hearts.…”

Section: Spontaneous Frequencymentioning

confidence: 99%

Temperature dependence of spontaneous electrical activity in early embryonic heart monitored optically with a potential-sensitive dye.

et al. 1983

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“…Recordings of the transmembrane potentials from non-pacemaker and pacemaker areas were taken by the intracellular microelectrode. The recording electrode , less than 1 µ in diameter, drawn from special glass capillary tubing and filled with 3 M-KCI , having low resistance (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), was selected and fixed on the arm of a micromanipulator . A chloride silver wire was pushed into the shaft of the electrode and was soldered onto flexible strand thereby connected to a d.c. amplifier with an input cathode follower of low grid current and reduced grid-earth capacity.…”

Section: Methodsmentioning

confidence: 99%

Effect of Anticholinesterases on the Membrane Potential of the Rabbit Heart

Takaori

Inoki

Toda

et al. 1961

Japanese Journal of Pharmacology

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We have already reported on the action of drugs such as acetylcholine, epinephrine, reserpine, cardiac glycosides, antiarrhythmic agents, veratrine and chelating agents on the intracellular action potentials recorded from the ventricular muscle, sinoatrial node and the non-pacemaker atrial fibers of the isolated rabbit heart using the ultramicro electrode method (1-3).The present experiments were undertaken to determine the influences of anticholin esterases on the membrane potentials of the isolated rabbit atrial muscle exhibiting spon taneous beating. Observations on the antagonisms between the action of anticholinesterases and of cholinesterase reactivators on the membrane potentials were also designed . METHODSAs described previously (1-3), the experiments were carried out on the right incised atrium of rabbits. The endocardial surface of the dissected right incised atrium was pinned on a cork block mounted in a lucite chamber containing about 10 ml of oxygen saturated Tyrode solution at approximately 30°C. The drugs were added directly to Tyrode solution in the chamber and their concentrations were expressed in terms of w/v (g/ml). Recordings of the transmembrane potentials from non-pacemaker and pacemaker areas were taken by the intracellular microelectrode. The recording electrode , less than 1 µ in diameter, drawn from special glass capillary tubing and filled with 3 M-KCI , having low resistance (8-20 MQ), was selected and fixed on the arm of a micromanipulator . A chloride silver wire was pushed into the shaft of the electrode and was soldered onto flexible strand thereby connected to a d.c. amplifier with an input cathode follower of low grid current and reduced grid-earth capacity. The output of the amplifier was led to a cathode-ray oscilloscope and ink-writing recorder (Sanei's Type MPA-204) .

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“…Jean Marshall (29), when working at Johns Hopkins later, was able to explain this by showing that on cooling below 20° C the transmembrane potential gradually fell, and when it was below 60 m V impulses were no longer propagated, and therefore contrac tions ceased. However when acetylcholine was added, the transmembrane potential rose and propagation of impulses began again.…”

Section: Burnmentioning

confidence: 99%

Essential Pharmacology

Burn

1969

Annu. Rev. Pharmacol.

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The beginning.-When I went to Cambridge in 1909, I became a member of Emmanuel College, of which John Harvard had been a member some 300 years earlier. I had the good fortune to have as a tutor, whose duty it was to supervise my progress in a general way, a man called F. G. Hopkins! He was the pioneer biochemist who isolated tryptophan, who worked on what he called "accessory food factors," and discovered glutathione. He was one of the most modest, gracious and graceful men I have ever known.At Cambridge I expected to make Chemistry my principal subject, but I found that for the first two years, I had to read Physics and two other sub jects as well. With Hopkins' approval Physiology and Botany were chosen.I discovered that, compared with those who taught Physiology and Physics, the chemists were an unattractive lot, particularly those who demonstrated in the practical class. These seemed very undistinguished, had cheerless faces and wore rather shabby clothes. The physiologists and the physicists had far more personality. In Physics I heard lectures given by J. J. Thom son, who seemed to be not of this world, and one of the demonstrators was Aston who built the first mass spectrograph. In Physiology the Professor was Langley, always well-dressed, who rode to hounds (that is he hunted the fox) and spoke to no one so far as I could see. There was W. B. Hardy who, when not studying the behaviour of colloids, sailed a yacht in the rough seas of the English Channel. In the summer he pinned a notice on his door which said "Back tomorrow." Joseph Barcroft, an Ulsterman, also sailed boats, and Keith Lucas, who was in part an engineer, had a large motor vessd on the Cam. Hidden in his room was a young man called Adri an, who spent his spare time at one period painting pictures in the style of Marinetti for an exhibition. It is said that all the works were sold, mostly to eager, but unwary Faculty members.Then there was the striking athletic figure of A. V. Hill, the very distin guished-looking W. M. Fletcher, and finally, helping Barcroft and Hopkins to fill the place with humanity, was H. K. Anderson.To enter the Cambridge world after leaving the school world was like meeting sea breezes on the shore after being confined in a stuffy room, and for two years I was mainly interested in debates and discussions of politics, religion and literature, which at least taught me to form my own opinions. After my third year I began research with Barcroft on two small prob lems concerned with the oxygen capacity of haemoglobin. I learnt (to my surpr ise) that I could work accurately, but I was still only half-interested and was occupied with other things. I was secretary of a University Social Discussion Society, and of its Inner Ci rc le which invited di stingui shed public figures to address them. One of these figures I remember was Bea� trice Webb who had encyclopaedic knowledge .on the reform of the Poor Law. I may say that she stayed, unforgett ably, at the ho use of Mr. W. C. Dampier Whetham, and that he lived at Upwater Lodge...

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Effects of Low Temperatures on Transmembrane Potentials of Single Fibers of the Rabbit Atrium

Cited by 73 publications

References 7 publications

Temperature dependence of spontaneous electrical activity in early embryonic heart monitored optically with a potential-sensitive dye.

Temperature dependence of spontaneous electrical activity in early embryonic heart monitored optically with a potential-sensitive dye.

Effect of Anticholinesterases on the Membrane Potential of the Rabbit Heart

Essential Pharmacology

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