Effects of &lt;i&gt;Azadirachta indica&lt;/i&gt; leaf aqeous extract on the antioxidant enzymes in paracetamol-induced hepatotoxicity in Wistar rats

Nwobodo, EI; Nwosu, Dennis C.; Ogbodo, Sylvester Ogbonna; Ugwuene, Francis Onukwube; Manafa, Patrick O.; Ani, Nnenna Onuabuchi; Johnkennedy, Nnodim; Ani, Okwudili

doi:10.4314/ijbcs.v12i1.1

Cited by 14 publications

(17 citation statements)

References 24 publications

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“…Most hepatic pathologies are associated with elevated levels of ALP and other enzymes that include AST and ALT. Decreased in ALP levels in this study may therefore be associated with hepatoprotective function which has been demonstrated for A. indica in previous studies (Nwobodo et al, 2018). Bilirubin, albumin, and globulin were not significantly different from control.…”

Section: Discussionsupporting

confidence: 82%

Evaluation of the toxic effects of the aqueous extract of Niprineem tea in mice and rats

Adigwe¹,

John-Africa²,

Adzu³

et al. 2022

Int. J. Bio. Chem. Sci

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Azadirachta indica is an important plant in traditional complementary and alternative medicine with decoctions (tea) being a common mode of administration. Herbal teas are frequently self-administered thus the need to prepare a standardized dosage form for the administration of such decoctions. The leaf of Azadirachta indica was formulated for administration as tea; thus, this study was designed to determine the safety profile of Niprineem tea. Oral acute and sub-chronic toxicity studies of the aqueous extract of Niprineem tea (NTE) were evaluated. The OECD (No 423) limit test was followed to determine the LD50 in Swiss albino mice, while OECD 407 guideline was used for the sub-chronic toxicity studies in Wistar rats. Acute administration of NTE did not cause detectable signs of toxicity in treated animals and no mortality was recorded. In the 28-day toxicity tests, there were no significant (p<0.05) changes in food and water intake, or urine and faecal output. Haematological analysis did not show deleterious effects in treated rats. Biochemical evaluation of indicators for renal and hepatic functions did not show significant changes after treatment with NTE. Likewise, histological tests did not result in structural changes in cells of the tissues of major organs. The results obtained suggest that Niprineem tea is relatively non-toxic and safe at the tested dose.

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Section: Discussionsupporting

confidence: 82%

Evaluation of the toxic effects of the aqueous extract of Niprineem tea in mice and rats

Adigwe¹,

John-Africa²,

Adzu³

et al. 2022

Int. J. Bio. Chem. Sci

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“…Histopathological findings from different resources showed that AzadirachtaIndica and Vitamin E has also a positive influence on biochemical parameters. The defensive alterations to cellular injury are reflected in the healing activity found in liver cells of animals treated with paracetamol 21,22 .…”

Section: Discussionmentioning

confidence: 99%

Antioxidative Role of Azadirachta Indica Vit. E in Restoration of Histopathological changes in Acetaminophen induced liver

Sodha¹,

Abbasi²,

Mawani³

et al. 2021

PJMHS

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Aim: To study the comparative effect of Acetaminophen induced liver toxicity with aqueous extract of Azadirachta Indica (Neem) and vitamin E on the basis of liver Histopathology. Methods: Sixty Wistar Rats of both sexes were split into four groups. Each group contained 15 animals. The control group was group A, Group B was treated orally with single dose of Paracetamol 2 mg / kg by weight, Group C was administrated orally with aqueous Neem extract 500 mg/kg +2 mg/Kg by weight oral Paracetamol, and Group D was given orally paracetamol+Neem extract+Vitamin E with 100mg/Kg/body weight for 15 days. Rats from all groups were decapitated, the liver was sliced, and liver tissues were taken for histological examination. Tissue samples were fixed in 10% formaldehyde, embedding in paraffin followed by Hematoxylin and Eosin dye (H&E) and observed under 400x magnification with a digital microscope. Results: On Histopathological examination of the rat`s liver in we found that the control group had a normal appearance, colour, and uniform surface without any necrosis. Group B showed severe necrosis and haemorrhagic patches. In comparison, Group C revealed normal appearance, colour, and smooth surface with no necrotic alterations. Livers from the group D looked virtually normal in terms of colour, undersurfaces, and organ weight. However, hepatoprotective effects were observed in the Group C and D. Therefore, we can conclude that Azadirachta indica and Vitamin E could serve as a good medication for defence against liver injury. Conclusion: Our findings showed thatextract of Azadirachta Indica and Vitamin E exhibited hepatoprotective effects on the Wistar rats that were subjected to Acetaminophen. Key words: Azadirachta Indica leaf extract, Vitamin E, hepatoprotective Paracetamol, Wistar rats.

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“…Des valeurs proches de l'activité des peroxydases de Curculigo pilosa et Stylochyton hypogaeus ont été observées dans la racine de Daucus carota (Santos de Araujo et al, 2004). Quant à B. bengeri, son activité peroxydasique est proche de celle de la glutathion-peroxydase obtenu par Nwobodo et al (2018).…”

Section: Discussionunclassified

Etude comparative des peroxydases de dix (10) plantes supérieures couramment rencontrées au Burkina Faso

Diao¹,

Dembele²,

Konaté³

et al. 2020

Int. J. Bio. Chem. Sci

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Cette étude vise à caractériser les peroxydases de dix plantes locales à savoir Allium cepa, Brachystelma bengeri, Brassica rapa, Curculigo pilosa, Daucus carota, Phaseolus vulgaris, Sorghum bicolor, Stylochyton hypogaeus, Urginea glaucescens et Xanthosoma sagittifolium afin de trouver une alternative au raifort, l'unique source de peroxydase commerciale. Pour cela, les protéines totales ont été extraites et l'activité enzymatique a été déterminée en utilisant le gaïacol comme substrat. L'effet du pH et de la température a été étudié ainsi que les paramètres cinétiques des peroxydases. Les résultats ont montré que Brassica rapa présente la plus forte activité peroxydasique (173,3 U/mg). Les pHs optima varient entre 4,5 et 6 ; les températures optimales varient entre 30 °C et 40 °C. L'étude de thermostabilité a montré que les pertes d'activité des peroxydases augmentent à la fois avec la température et la durée de chauffage. Les études cinétiques ont montré que l'affinité des peroxydases pour le gaïacol et le peroxyde d'hydrogène de même que les vitesses maximales sont plus élevées respectivement avec les peroxydases de Brassica rapa et Xanthosoma sagittifolium comparativement aux autres. Ainsi les peroxydases de Brassica rapa, Sorghum bicolor et Daucus carota se sont révélées intéressantes et comme une alternative pour d'éventuelle application.

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Effects of <i>Azadirachta indica</i> leaf aqeous extract on the antioxidant enzymes in paracetamol-induced hepatotoxicity in Wistar rats

Cited by 14 publications

References 24 publications

Evaluation of the toxic effects of the aqueous extract of Niprineem tea in mice and rats

Evaluation of the toxic effects of the aqueous extract of Niprineem tea in mice and rats

Antioxidative Role of Azadirachta Indica Vit. E in Restoration of Histopathological changes in Acetaminophen induced liver

Etude comparative des peroxydases de dix (10) plantes supérieures couramment rencontrées au Burkina Faso

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