Effects of Lunar Soil Simulant on Systemic Oxidative Stress and Immune Response in Acute Rat Lung Injury

Sun, Yanling; Liu, Jinguo; Kong, Yuanyuan; Hu, Jiasen; Zhang, Xiaoping

doi:10.3923/ijp.2018.766.772

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Low levels of GSH may lead to an imbalance between oxidants and antioxidants in lung tissue, enhancing inflammatory responses and worsening lung damage. Glutathione levels were elevated in this study, which is different from our previous findings 5 . We speculate that the pattern and duration of simulated lunar dust exposure may have been different in the studies, causing this discrepancy.…”

Section: Discussioncontrasting

confidence: 99%

“…Sun et al exposed rats to two doses of lunar dust simulant by tracheal instillation, and measured related immune and oxidative indicators in bronchoalveolar lavage fluid (BALF) of rats. The results confirmed that under the effect of simulated lunar dust, inflammation, oxidative stress and immune response promote each other, causing pulmonary vascular endothelial cell damage and lung inflammation, and inducing lung injury 4–6 . However, the pathogenesis of lung injury induced by simulated lunar dust has not been clearly elucidated.…”

Section: Introductionsupporting

confidence: 53%

“…Glutathione levels were elevated in this study, which is different from our previous findings. 5 We speculate that the pattern and duration of simulated lunar dust exposure may have been different in the studies, causing this discrepancy. It is generally believed that depletion of GSH in tissue cells in the initial stage of oxidative stress reduces the level of GSH, and then due to the feedback regulation of GSH and the stimulation of internal and external oxides, the expression of γ-GCS gene in the airway wall and lung epithelial cells is enhanced, and the synthesis of GSH is increased.…”

Section: Discussionmentioning

confidence: 84%

“…The results confirmed that under the effect of simulated lunar dust, inflammation, oxidative stress and immune response promote each other, causing pulmonary vascular endothelial cell damage and lung inflammation, and inducing lung injury. [4][5][6] However, the pathogenesis of lung injury induced by simulated lunar dust has not been clearly elucidated. Therefore, more in-depth studies are needed to better understand the pathogenic process of lunar dust simulants exposure leading to pulmonary toxicity.…”

mentioning

confidence: 99%

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Exploring the mechanism of lung injury induced by lunar dust simulant in rats based on metabolomic analysis

Gu,

Yin,

Sun

et al. 2023

Environmental Toxicology

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Inflammatory response and oxidative stress are considered to be important mechanisms of lung injury induced by lunar dust. However, the pulmonary toxicological mechanism remains unclear. In the present study, Wistar rats were exposed to CLDS‐i 7 days/week, 4 h/day, for 4 weeks in the mouth and nose. Lung tissue samples were collected for histopathological analysis and ultra‐performance liquid chromatography–mass spectrometry analysis. Enzyme activities and expression levels of key metabolic enzymes were detected by biochemical analysis and real‐time PCR. The pathological features of lung tissue showed that CLDS‐i caused congestion and inflammation in the lungs, and the lung structure was severely damaged. Metabolomics analysis showed that 141 metabolites were significantly changed in the lung tissue of the CLDS‐i group compared with the control group. Combined with Kegg pathway analysis, it was found that the changes of amino acid metabolites were involved in these pathways, indicating that the simulated lunar dust exposure had the most obvious effect on amino acid metabolism in the lung tissue of rats. Real‐time PCR analysis showed that the mRNA expression of six key enzymes related to amino acid metabolism was changed, and the enzyme activities of these key enzymes were also changed, which were consistent with the results of qPCR. These results suggest that changes in amino acid metabolism may be closely related to the pathogenesis of lung injury induced by lunar dust, and amino acid metabolism may be a potential biomarker of lung diseases related to lunar dust exposure.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

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Exploring the mechanism of lung injury induced by lunar dust simulant in rats based on metabolomic analysis

Gu,

Yin,

Sun

et al. 2023

Environmental Toxicology

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“…Interleukin 6 (IL-6) and TNF-α are two pro-inflammatory cytokines that are increased in in vitro studies with lunar dust simulant (LDS), which can lead to chronic inflammation [45]. TNF-α is produced by the lungs epithelial cells in the presence of silica nanoparticles [46] or in response to exposure to smoke particles [47].…”

Section: Immunological Adverse Effectsmentioning

confidence: 99%

A Dusty Road for Astronauts

Miranda,

Marchal,

Cumps

et al. 2023

Biomedicines

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The lunar dust problem was first formulated in 1969 with NASA’s first successful mission to land a human being on the surface of the Moon. Subsequent Apollo missions failed to keep the dust at bay, so exposure to the dust was unavoidable. In 1972, Harrison Schmitt suffered a brief sneezing attack, red eyes, an itchy throat, and congested sinuses in response to lunar dust. Some additional Apollo astronauts also reported allergy-like symptoms after tracking dust into the lunar module. Immediately following the Apollo missions, research into the toxic effects of lunar dust on the respiratory system gained a lot of interest. Moreover, researchers believed other organ systems might be at risk, including the skin and cornea. Secondary effects could translocate to the cardiovascular system, the immune system, and the brain. With current intentions to return humans to the moon and establish a semi-permanent presence on or near the moon’s surface, integrated, end-to-end dust mitigation strategies are needed to enable sustainable lunar presence and architecture. The characteristics and formation of Martian dust are different from lunar dust, but advances in the research of lunar dust toxicity, mitigation, and protection strategies can prove strategic for future operations on Mars.

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Mechanisms involved in inflammatory pulmonary fibrosis induced by lunar dust simulant in rats

Sun

Liu

Zhang

et al. 2018

Environmental Toxicology

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Lunar dust is one of the biggest risk factors in the future manned exploration mission. Much is not known about the pulmonary toxicity of lunar dust. The aim of this study was to evaluate the lung inflammation and oxidative stress induced by subacute exposure to lunar dust stimulant (LDS) in rats. Wistar rats were intratracheally administered LDS, twice a week for 3 weeks. Inflammatory cell counting and cytokine assays using bronchoalveolar lavage fluid (BALF) were performed. Lung tissues were processed for histopathological examination and immunohistochemical staining. Biomarkers of oxidative stress and genes and proteins related to inflammation and fibrosis in lung tissue were also determined. The neutrophil count in the BALF of LDS‐exposed groups was higher than that in controls (P < .05). LDS caused a significant increase in some of biochemical indicators and proinflammatory factors levels in BALF compared with control group. The normal balance between oxidation and antioxidation was broken by LDS. Pathological characteristics of lung tissue and immunohistochemical results for α‐smooth muscle actin (α‐SMA) indicated that inflammatory response was an extremely important passage to pulmonary fibrosis. Real‐time PCR analysis showed elevated levels of nitric oxide synthase (NOS) and nicotinamide adenine dinucleotide phosphate oxidase (NOX) mRNA in the lungs (P < .05). Western blotting results were consistent with immunohistochemistry and qPCR results. These results indicate that inhalation of lunar dust may cause inflammatory pulmonary fibrosis. NOX4 may be a key potential therapeutic target for inflammatory injury and fibrosis in the lung.

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Effects of Lunar Soil Simulant on Systemic Oxidative Stress and Immune Response in Acute Rat Lung Injury

Cited by 6 publications

References 24 publications

Exploring the mechanism of lung injury induced by lunar dust simulant in rats based on metabolomic analysis

Exploring the mechanism of lung injury induced by lunar dust simulant in rats based on metabolomic analysis

A Dusty Road for Astronauts

Mechanisms involved in inflammatory pulmonary fibrosis induced by lunar dust simulant in rats

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