Effects of Lung Surfactant Proteins, SP-B and SP-C, and Palmitic Acid on Monolayer Stability

Ding, Junqi; Takamoto, Dawn Y.; Nahmen, A. von; Lipp, Michael M.; Lee, Ka Yee C.; Waring, Alan J.; Zasadzinski, Joseph A.

doi:10.1016/s0006-3495(01)76198-x

Cited by 168 publications

(287 citation statements)

References 44 publications

Supporting

Mentioning

266

Contrasting

Unclassified

Order By: Relevance

“…In contrast to SP-A and SP-D, the small and extremely hydrophobic proteins SP-B and SP-C are essential components during formation of surfactant monolayers and stabilisation of air-fluid interfaces [5,6]. Similar to SP-A and SP-D, the presence of SP-B and SP-C has already been demonstrated in a variety of tissues and humours, including tissues of the nasolacrimal apparatus and ocular surface, in tear fluid, in salivary glands, in the gingiva and nasal mucosa [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation

et al. 2014

View full text Add to dashboard Cite

The lung constantly interacts with numerous pathogens. Thus, complex local immune defence mechanisms are essential to recognise and dispose of these intruders. This work describes the detection, characterisation and three-dimensional structure of a novel protein of the lung (surfactantassociated protein 3 (SFTA3/SP-H)) with putative immunological features.Bioinformatics, biochemical and immunological methods were combined to elucidate the structure and function of SFTA3. The tissue-specific detection and characterisation was performed by using electron microscopy as well as fluorescence imaging.Three-dimensional structure generation and analysis led to the development of specific antibodies and, as a consequence, to the localisation of a novel protein in human lung under consideration of cystic fibrosis, asthma and sepsis. In vitro experiments revealed that lipopolysaccharide induces expression of SFTA3 in the human lung alveolar type II cell line A549. By contrast, the inflammatory cytokines interleukin (IL)-1b and IL-23 inhibit expression of SFTA3 in A549. Sequence-and structure-based prediction analysis indicated that the novel protein is likely to belong to the family of lung surfactant proteins.The results suggest that SFTA3 is an immunoregulatory protein of the lung with relevant protective functions during inflammation at the mucosal sites. @ERSpublications SFTA3: a novel lung protein with putative protective and immunological functions during inflammation in lung diseases

show abstract

Section: Introductionmentioning

confidence: 99%

SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation

et al. 2014

View full text Add to dashboard Cite

show abstract

“…SP-B and SP-C cationic lung surfactant can reduce fluidity and the anionic charge density of the monolayer domain through the docking of surfactants from the subphase into the monolayer 41 . In mixed monolayers, the electrostatic forces between the cationic proteins and anionic lipids can reduce those between lipids, resulting in the reintroduction of giant folds into the system; therefore, the materials are conserved.…”

Section: Topography Of Collapsementioning

confidence: 99%

Collapsed States of Langmuir Monolayers

2016

View full text Add to dashboard Cite

“…We expect that the approach presented here can be extended to quantify the distribution of a variety of labeled proteins, polymers, and other materials of interest in the vicinity of monolayers at the air-water interface, using confocal microscopy (34)(35)(36)(37). The method may also be amenable to determining local fluorophore concentrations near liquid-liquid and liquid-solid interfaces to further address issues of ion distributions and modifications to Gouy-Chapman theory (38).…”

mentioning

confidence: 99%

Visualizing monolayers with a water-soluble fluorophore to quantify adsorption, desorption, and the double layer

Shieh

Zasadzinski

2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Contrast in confocal microscopy of phase-separated monolayers at the air-water interface can be generated by the selective adsorption of water-soluble fluorescent dyes to disordered monolayer phases. Optical sectioning minimizes the fluorescence signal from the subphase, whereas convolution of the measured point spread function with a simple box model of the interface provides quantitative assessment of the excess dye concentration associated with the monolayer. Coexisting liquid-expanded, liquid-condensed, and gas phases could be visualized due to differential dye adsorption in the liquid-expanded and gas phases. Dye preferentially adsorbed to the liquid-disordered phase during immiscible liquid-liquid phase coexistence, and the contrast persisted through the critical point as shown by characteristic circle-to-stripe shape transitions. The measured dye concentration in the disordered phase depended on the phase composition and surface pressure, and the dye was expelled from the film at the end of coexistence. The excess concentration of a cationic dye within the double layer adjacent to an anionic phospholipid monolayer was quantified as a function of subphase ionic strength, and the changes in measured excess agreed with those predicted by the mean-field Gouy-Chapman equations. This provided a rapid and noninvasive optical method of measuring the fractional dissociation of lipid headgroups and the monolayer surface potential.lipid domains | lung surfactants | surface potential | phase behavior L ipid monolayers have long been used as a simplified model for cell membranes (1-4), especially for the study of 2D phase separation that may underlie the raft hypothesis of cell membrane organization (5-8). Monolayers are also of interest in the physics of ordering and dynamic processes in two dimensions such as the structure and flow of hexatic phases (9-11). Additionally, all air-breathing mammals have a lipid-protein monolayer lining the lung alveoli to minimize surface tension effects on breathing; deficiency or disruption of this monolayer leads to potentially fatal respiratory distress syndrome in infants and adults (12, 13). The general acceptance of complex monolayer and bilayer phase behavior, especially phase coexistence (14-16) and critical phenomena (7,8,17,18), has relied on the visualization of fluorescently tagged lipids segregated between phases in the monolayer. This segregation, due to differences in local molecular organization, provides the necessary contrast to visualize even subtle differences in packing density, molecular tilt, and short-and long-range ordering. Since the technique was introduced (14,(19)(20)(21), visualization of the distribution of labeled lipids has settled numerous debates over the molecular organization of monolayers (2, 4, 22, 23) and bilayers (8, 24).However, fluorescently tagged lipids, like many of the other lipids in monolayers and bilayers, are effectively insoluble in the surrounding aqueous subphase and are trapped in the monolayer or bilayer. As a result, expelling ...

show abstract

Effects of Lung Surfactant Proteins, SP-B and SP-C, and Palmitic Acid on Monolayer Stability

Cited by 168 publications

References 44 publications

SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation

SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation

Collapsed States of Langmuir Monolayers

Visualizing monolayers with a water-soluble fluorophore to quantify adsorption, desorption, and the double layer

Contact Info

Product

Resources

About