eCM 2016
DOI: 10.22203/ecm.v032a05
|View full text |Cite
|
Sign up to set email alerts
|

Effects of macrophage-activating lipopeptide-2 (MALP-2) on the vascularisation of implanted polyurethane scaffolds seeded with microvascular fragments

Abstract: The seeding of scaffolds with adipose tissue-derived microvascular fragments represents a promising strategy to establish a sufficient blood supply in tissue constructs. Herein, we analysed whether a single application of macrophage-activating lipopeptide-2 (MALP-2) at the implantation site further improves the early vascularisation of such microvessel-seeded constructs. Microvascular fragments were isolated from epididymal fat pads of C57BL/6 mice. The fragments were seeded on polyurethane scaffolds, which we… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
12
0

Year Published

2017
2017
2022
2022

Publication Types

Select...
7

Relationship

2
5

Authors

Journals

citations
Cited by 13 publications
(12 citation statements)
references
References 34 publications
0
12
0
Order By: Relevance
“…pericytes and smooth muscle cells (Wang et al, 2011), and its expression is upregulated by hypoxia via mitogen-activated protein kinase (MAPK;Cokic et al, 2014). Freshly isolated ad-MVF lack their own blood perfusion and, thus, suffer from hypoxia and oxidative stress (Grässer et al, 2016). Accordingly, it may be speculated that they particularly benefit from EPO treatment in the initial phase after their isolation.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…pericytes and smooth muscle cells (Wang et al, 2011), and its expression is upregulated by hypoxia via mitogen-activated protein kinase (MAPK;Cokic et al, 2014). Freshly isolated ad-MVF lack their own blood perfusion and, thus, suffer from hypoxia and oxidative stress (Grässer et al, 2016). Accordingly, it may be speculated that they particularly benefit from EPO treatment in the initial phase after their isolation.…”
Section: Discussionmentioning
confidence: 99%
“…These strategies focus on the generation of preformed microvascular networks within the constructs, which rapidly interconnect They also contain a large number of mesenchymal stem cells within their physiological niche (McDaniel et al, 2014;Frueh et al, 2017a). Accordingly, ad-MVF exhibit a unique regenerative potential and a high capacity to reassemble in vivo into blood-perfused microvascular networks within implanted tissue constructs (Shepherd et al, 2007;Grässer et al, 2016;Frueh et al, 2017a). However, the latter process still requires several days, resulting in an initial lack of blood supply to the implants (Laschke et al, 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Dorsal skinfold chambers were implanted in wild-type C57BL/6 mice (Institute for Clinical and Experimental Surgery, Saarland University, Homburg, Germany), with an age of 4-6 months and a body weight of 24-28 g. Epididymal fat was isolated from male green fluorescent protein (GFP) + mice [C57BL/6-Tg(CAG-EGFP)1Osb/J; The Jackson Laboratory, Bar Harbor, ME, USA], with an age of 7-12 months and a body weight of > 30 g, to guarantee large epididymal fat pads containing sufficient amounts of ad-MVF for the seeding of the matrices (Grässer et al, 2016). The animals were housed under a 12 h light/dark cycle and received ad libitum water and standard food pellets (Altromin, Lage, Germany).…”
Section: Animalsmentioning
confidence: 99%
“…For this purpose, adipose tissue‐derived microvascular fragments (ad‐MVF) have been suggested as native vascularization units (Laschke & Menger, ; Pilia et al, ). Similar to stromal vascular fraction single cells, they can be isolated in large amounts by enzymatic digestion of fat samples and seeded onto different types of scaffolds (Frueh, Später, Lindenblatt, et al, , Frueh, Später, Scheuer, Menger, & Laschke, , Grässer et al, ; Gruionu, Stone, Schwartz, Hoying, & Williams, ). However, the digestion time for the isolation of ad‐MVFs is much shorter (~10 min; Frueh, Später, Lindenblatt, et al, ) than that for the isolation of stromal vascular fraction single cells (~45–60 min; Parra, Serra, & Palou, ; Weisberg et al, ).…”
Section: Introductionmentioning
confidence: 99%