Effects of magnetic fields on the enzymatic synthesis of naringin palmitate

Zhang, Xia; Wan, Liting; Li, Lin; Xu, Zhenbo; Su, Jianyu; Li, Bing; Huang, Jianrong

doi:10.1039/c8ra01441h

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…When H was >0.5 kA/m, the MIC activity under the AMF showed a decreasing trend with increasing intensity but was steady under the SMF, which may be due to the alteration of the conformation of MIC and therefore affecting the active site of enzymes. 30,31 By contrast, at 20 °C and in the chitosan solution with higher viscosity, the reciprocating vibration of MIC particles caused by the AMF would facilitate the magnetic agglomeration, resulting in poor contact between the substrate and enzymes compared to the SMF. As the temperature increased to 50 °C, the MIC activities under the SMF and AMF both gradually increased as the magnetic field intensity increased from 0 to 2.5 kA/m and then decreased dramatically as the magnetic field intensity further increased to 5.0 kA/m.…”

Section: Resultsmentioning

confidence: 99%

Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field

Cui

Kou

et al. 2018

Ind. Eng. Chem. Res.

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A novel, magnetic three-phase fluidized bed reactor (MTFBR) was designed with magnetic immobilized cellulase (MIC) as the biocatalyst for preparing chitooligosaccharides from chitosan. The MIC showed higher enzyme activity in a pulse magnetic field (PMF) than that in a steady magnetic field (SMF) or alternating magnetic field (AMF) under certain operating parameters. The application of a magnetic field increased the maximum reaction rate (V max ) and Michaelis constant (K m ), and the reaction rates of the MIC-catalyzed reaction in PMF and SMF at high concentration of chitosan solution (C s , 5 < C s < 20 mg/mL) increased as the intensity of the magnetic field increased. The concentrations of chitosan pentamer and hexamer produced were higher in the PMF than in the SMF and AMF, and the application of a magnetic field greatly reduced (by as much as 37.5%) the biocatalytic reaction time required to reach the maximum concentration of the desired chitooligosaccharides. These results suggest that the customized MTFBR supplemented with MIC is a reusable and effective piece of biocatalytic equipment, indicating that it has potential applications for large industrial-scale chitooligosaccharide syntheses.

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Section: Resultsmentioning

confidence: 99%

Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field

Cui

Kou

et al. 2018

Ind. Eng. Chem. Res.

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Section: Different Triggering Methodsmentioning

confidence: 99%

Section: Different Triggering Methodsmentioning

confidence: 99%

“…Analogously, Zhang and coauthors investigated the effects of a magnetic field on the enzymatic synthesis of naringin palmitate catalyzed by C. antarctica lipase B under different magnetic conditions. 89 The poor solubility and low stability of naringin, a flavanone glycoside, limit its use. 90 Therefore, the lipophilicity of naringin is changed by introducing aliphatic groups into the structure of naringin by enzymatic esterification.…”

Section: Effect Of Magnetic Fieldmentioning

confidence: 99%

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Propelling of Enzyme Activity by Using Different Triggering Strategies: Applications and Perspectives

Dik,

Bakar,

Ulu

et al. 2023

Ind. Eng. Chem. Res.

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“…The encapsulation efficiency obtained, 30-40%, is due to the poor solubility of NAR both in water and lipid phases, which leads to low rates of encapsulation [34][35][36]. Nevertheless, the loaded amount is protected from extensive metabolism and decomposition in the body, in opposition to what happens with free NAR [9,10,[13][14][15].…”

Section: Nanoparticles Characterizationmentioning

confidence: 99%

Empowering Naringin’s Anti-Inflammatory Effects through Nanoencapsulation

Marinho,

Seabra,

Lima

et al. 2024

IJMS

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Abundant in citrus fruits, naringin (NAR) is a flavonoid that has a wide spectrum of beneficial health effects, including its anti-inflammatory activity. However, its use in the clinic is limited due to extensive phase I and II first-pass metabolism, which limits its bioavailability. Thus, lipid nanoparticles (LNPs) were used to protect and concentrate NAR in inflamed issues, to enhance its anti-inflammatory effects. To target LNPs to the CD44 receptor, overexpressed in activated macrophages, functionalization with hyaluronic acid (HA) was performed. The formulation with NAR and HA on the surface (NAR@NPsHA) has a size below 200 nm, a polydispersity around 0.245, a loading capacity of nearly 10%, and a zeta potential of about 10 mV. In vitro studies show the controlled release of NAR along the gastrointestinal tract, high cytocompatibility (L929 and THP-1 cell lines), and low hemolytic activity. It was also shown that the developed LNPs can regulate inflammatory mediators. In fact, NAR@NPsHA were able to decrease TNF-α and CCL-3 markers expression by 80 and 90% and manage to inhibit the effects of LPS by around 66% for IL-1β and around 45% for IL-6. Overall, the developed LNPs may represent an efficient drug delivery system with an enhanced anti-inflammatory effect.

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Effects of magnetic fields on the enzymatic synthesis of naringin palmitate

Abstract: Immobilized CALB (I-CALB) and I-CALB solution was treated by magnetic fields and enzymatic reactions with them were compared.

Cited by 9 publications

References 31 publications

Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field

Comparing Immobilized Cellulase Activity in a Magnetic Three-Phase Fluidized Bed Reactor under Three Types of Magnetic Field

Propelling of Enzyme Activity by Using Different Triggering Strategies: Applications and Perspectives

Empowering Naringin’s Anti-Inflammatory Effects through Nanoencapsulation

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