Effects of malachite green (MG) and its major metabolite, leucomalachite green (LMG), in two human cell lines

Stammati, Annalaura; Nebbia, Carlo; Angelis, De; Albo, A. Giuliano; Carletti, Monica; Rebecchi, Claudia; Zampaglioni, F.; Dacasto, Mauro

doi:10.1016/j.tiv.2005.06.021

Cited by 154 publications

(64 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, other researchers have demonstrated toxicity potential of these compounds in cell lines, such as mercuric compounds in human fetal hepatic cell line WRL-68, 57 potassium dichromate in HepG2 cells, 58 and malachite green oxalate in HepG2 and Caco-2 cell lines. 59 Cytotoxicity has also been demonstrated with several of the compounds in primary cells. Staurosporine has been shown to induce apoptosis in murine hepatocytes 60 and has been used as a positive control in human hepatocytes for HCS of hepatocellular toxicity markers.…”

Section: Cytotoxicity Of Chemical Compoundsmentioning

confidence: 99%

Assessment of Compound Hepatotoxicity Using Human Plateable Cryopreserved Hepatocytes in a 1536-Well-Plate Format

Moeller¹,

Shukla

Xia

2012

ASSAY and Drug Development Technologies

View full text Add to dashboard Cite

Hepatotoxicity is a major concern for both drug development and toxicological evaluation of environmental chemicals. The assessment of compound-induced hepatotoxicity has traditionally relied on in vivo testing; however, it is being replaced by human in vitro models due to an emphasis on the reduction of animal testing and species-specific differences. Since most cell lines and hybridomas lack the full complement of enzymes at physiological levels found in the liver, primary hepatocytes are the gold standard to study liver toxicities in vitro due to the retention of most of their in vivo activities. Here, we optimized a cell viability assay using plateable cryopreserved human hepatocytes in a 1536-well-plate format. The assay was validated by deriving inhibitory concentration at 50% values for 12 known compounds, including tamoxifen, staurosporine, and phenylmercuric acetate, with regard to hepatotoxicity and general cytotoxicity using multiple hepatocyte donors. The assay performed well, and the cytotoxicity of these compounds was confirmed in comparison to HepG2 cells. This is the first study to report the reliability of using plateable cryopreserved human hepatocytes for cytotoxicity studies in a 1536-well-plate format. These results suggest that plateable cryopreserved human hepatocytes can be scaled up for screening a large compound library and may be amenable to other hepatocytic assays such as metabolic or drug safety studies.

show abstract

Section: Cytotoxicity Of Chemical Compoundsmentioning

confidence: 99%

Assessment of Compound Hepatotoxicity Using Human Plateable Cryopreserved Hepatocytes in a 1536-Well-Plate Format

Moeller¹,

Shukla

Xia

2012

ASSAY and Drug Development Technologies

View full text Add to dashboard Cite

show abstract

“…MG and its derivatives have been reported to induce human carcinogenesis and mutagenesis. Several studies have also shown that exposure to this dye increases the risk of chromosomal fractures, reduces fertility and may act as an inhibitor of respiratory enzymes (Culp et al, 2006;Stammati et al, 2005). Due to the risk that MG imposes on fish consumers, its use in fishery applications is highly controversial.…”

Section: Introductionmentioning

confidence: 99%

Malachite green decolorization by the filamentous fungus Myrothecium roridum – Mechanistic study and process optimization

Jasińska

Paraszkiewicz

Sip

et al. 2015

Bioresource Technology

View full text Add to dashboard Cite

“…It is soluble and stable in water at high concentrations. While it is toxic to mammalian cells (Bose et al, 2004(Bose et al, , 2005Fessard et al, 1999;Gupta et al, 2003;Littlefield et al, 1985;Stammati et al, 2005), several species of fungi and bacteria are known to decolorize and degrade malachite green through a variety of pathways.…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium smegmatis mc2 155 fbiC and MSMEG_2392 are involved in triphenylmethane dye decolorization and coenzyme F420 biosynthesis

2007

View full text Add to dashboard Cite

Effects of malachite green (MG) and its major metabolite, leucomalachite green (LMG), in two human cell lines

Cited by 154 publications

References 27 publications

Assessment of Compound Hepatotoxicity Using Human Plateable Cryopreserved Hepatocytes in a 1536-Well-Plate Format

Assessment of Compound Hepatotoxicity Using Human Plateable Cryopreserved Hepatocytes in a 1536-Well-Plate Format

Malachite green decolorization by the filamentous fungus Myrothecium roridum – Mechanistic study and process optimization

Mycobacterium smegmatis mc2 155 fbiC and MSMEG_2392 are involved in triphenylmethane dye decolorization and coenzyme F420 biosynthesis

Contact Info

Product

Resources

About