Effects of mammalian gonadotropins (FSH and LH) on the testes of the lizard Anolis carolinensis

Licht, Paul; Pearson, Anita K.

doi:10.1016/0016-6480(69)90259-7

Cited by 84 publications

(31 citation statements)

References 14 publications

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“…Similar increases in relative potency are evident when this material is tested in other reptilian systems including ovulation in lizards (33) and steroid production by turtle ovaries, including the homologous species (32). The relative potency of the FSH in the Anolis assay (25-30 x NIH-FSH-S1) compares favorably with highly purified ovine FSH (34). Also, the turtle FSH is approximately 500 times more potent than crude alkaline extracts of the pituitary (6).…”

Section: Discussionmentioning

confidence: 49%

Isolation and Characterization of Follicle-Stimulating Hormone and Luteinizing Hormone and Its Subunits from Snapping Turtle (Chelydra serpentina) Pituitaries

1976

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Highly purified luteinizing hormone and follicle-stimulating hormone have been isolated from extracts of snapping turtle (Chelydra serpentina) pituitaries. Both hormones are potent in non-mammalian gonadotropin bioassays (1.8 X NIH-LH-S1 and 30 X NIH-FSH-S1). The materials have been characterized by polyacrylamide gel electrophoresis, amino terminal group analysis, amino acid and carbohydrate content, and, in the case of turtle luteinizing hormone, ultracentrifugation. The luteinizing hormone was shown to dissociate and subunits were prepared by the countercurrent distribution technique and characterized. Biological activity of the hormone could be regenerated by recombination of the subunits. In addition, it was shown that the snapping turtle luteinizing hormone subunits could be combined with subunits from ovine luteinizing hormone with generation of significant biological activity. Comparisons in properties of the turtle gonadotropins have been made with ovine gonadotropins, showing, in many cases, similarities in properties, suggesting structural features which have been conserved during evolution.

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Section: Discussionmentioning

confidence: 49%

Isolation and Characterization of Follicle-Stimulating Hormone and Luteinizing Hormone and Its Subunits from Snapping Turtle (Chelydra serpentina) Pituitaries

1976

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“…This level of LH contamination is at least four orders of magnitude less than the minimum level of LH ( > 25 mu.) found effective in the present and previous tests (Licht & Pearson, 1969a;Licht, 1970) on Anolis. Also, no synergism has been found between the two molecules in the gonadal responses of Anolis or other lizards (Licht & Pearson, 1969a;Jones, 1969;Licht, 1970;Reddy & Prasad, 1970a, 6).…”

Section: Physiological Actions Of Vertebrate Gonadotrophins In the LImentioning

confidence: 57%

“…NIH) in expts 1 and 2. However, some comparisons of these results with the standardized NIH-FSH and LH are possible since the latter have been studied at the same time of year and under the same conditions in this laboratory (Licht & Pearson, 1969a;Licht, 1970).…”

Section: Methodsmentioning

confidence: 96%

“…As one approach to this problem, gonadotrophins from several vertebrates (a mammal, the sheep, and a fish, the salmon) have been tested in the lizard Anolis carolinensis (Licht & Pearson, 1969a;Licht & Donaldson, 1969;Licht, 1970). With regard to reptilian endocrinology, the results of these studies have already cast some doubt on the generally held assump¬ tion that the reptiles possess two separate gonadotrophins, an FSH and LH.…”

Section: Introductionmentioning

confidence: 97%

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Actions of Mammalian, Avian and Piscine Gonadotrophins in the Lizard

Licht¹,

Hartree²

1971

Journal of Endocrinology

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The gonadotrophic activities of crude glycoprotein fractions from pituitaries of man, sheep, chicken and carp and of partially purified follicle\x=req-\ stimulating hormone (FSH) and luteinizing hormone (LH) from the three tetrapod species were examined in both sexes of the lizard Anolis carolinensis. The piscine material did not show activity in the lizard but all mammalian and avian preparations promoted spermatogenesis, ovarian growth, ovulation and steroidogenesis. FSH preparations were far more potent than LH, in fact the actions of the LH preparations may have been due largely to FSH contamination. These findings are consistent with earlier conclusions that FSH alone may be able to stimulate all types of gonadal activity in lizards, except that high doses of FSH block ovulation.Comparisons of the relative potencies of the avian and mammalian preparations provide evidence for the zoological specificity of vertebrate gonadotrophins. In general, the relative potency of chicken gonadotrophins in lizards was greater than that estimated from standard rodent bioassays: i.e. the lizard is relatively more sensitive to chicken gonadotrophin than the rodent.

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“…Male alligator puberty most likely is a neuroendocrine event, like that described in mammals and birds. In many reptiles, hypophysectomy leads to testicular degeneration [van Tienhoven, 1983], but exogenous gonadotropins restore spermatogenesis [Licht and Pearson, 1969]. Furthermore, gonadotropin or androgen treatments of immature male alligators induces testicular development [Forbes, 1937;Ramaswam and Jacob, 1965] and dramatic increases in plasma sex steroid hormone concentrations [Edwards et al, 2004].…”

mentioning

confidence: 99%

Gonadal mRNA Expression Levels of TGFβ Superfamily Signaling Factors Correspond with Post-Hatching Morphological Development in American Alligators

Moore

Hamlin

Botteri

et al. 2010

Sex Dev

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Paracrine factor signaling regulates many aspects of vertebrate gonadal development. We investigated key ovarian and testicular morphological markers of the American alligator (Alligator mississippiensis) during the first 5 months post-hatching and correlated gonadal development with mRNA expression levels of a suite of regulatory factors. In both sexes, we observed significant morphology changes, including ovarian follicle assembly and meiotic progression of testicular germ cells. Concomitant with these changes were sexually dimorphic and ontogenetically variable mRNA expressions. In ovaries, FOXL2, aromatase, and follistatin mRNA expression was greater than in testes at all ages. At one week after hatching, we observed ovarian medullary remodeling in association with elevated activin/inhibin βA subunit, follistatin, and aromatase mRNA expressions. Three and 5 months following hatching and concomitant with follicle assembly, ovaries showed increased mRNA expression levels of GDF9 and the mitotic factor PCNA. In testes, the activin/inhibin α and βB subunit transcript levels were greater than in ovaries at all ages. Elevated testicular expression of GDF9 mRNA levels at 5 months after hatching aligned with increased spermatogenic activity. We propose that the mRNA expression levels and concomitant morphological changes observed here affect the establishment of alligator reproductive health and later fertility.

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Effects of mammalian gonadotropins (FSH and LH) on the testes of the lizard Anolis carolinensis

Cited by 84 publications

References 14 publications

Isolation and Characterization of Follicle-Stimulating Hormone and Luteinizing Hormone and Its Subunits from Snapping Turtle (Chelydra serpentina) Pituitaries

Isolation and Characterization of Follicle-Stimulating Hormone and Luteinizing Hormone and Its Subunits from Snapping Turtle (Chelydra serpentina) Pituitaries

Actions of Mammalian, Avian and Piscine Gonadotrophins in the Lizard

Gonadal mRNA Expression Levels of TGFβ Superfamily Signaling Factors Correspond with Post-Hatching Morphological Development in American Alligators

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