Effects of Manduca sexta allatostatin and an analog on the pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) and degradation by enzymes from the aphid gut

Down, RE; Hj, Matthews; Audsley, Neil

doi:10.1016/j.peptides.2009.06.017

Cited by 22 publications

(15 citation statements)

References 45 publications

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“…A variety of insect neuropeptides and neuropeptide analogues have been shown to be insecticidal. These include the PISCF allatostatins (ASTs) and insect kinins which, when fed to aphids, cause significant mortality [23,38]. Others such as FGLa/ASTs and short neuropeptide F have been shown to regulate feeding and foraging behavior [33,55].…”

Section: Introductionmentioning

confidence: 99%

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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Tel: +44 (0)1904 4626282 Highlights  In silico analysis identified 28 peptide precursors  Peptidome analysis of adult brain, corpus cardiacum, thoracico-abdominal ganglion identified peptides from 15 different families.  Myosuppressin was present in all tissue analysed, and was the only peptide identified in the crop nerve bundle AbstractDrosophila suzukii is a highly polyphagous invasive pest which has been recently introduced into Europe and North America, where it is causing severe economic losses through larval infestations of stone and berry fruits. The peptidome of the selected nervous tissues of adult D. suzukii was investigated as a first step in identifying potential targets for the development of novel insecticides.Through in silico analyses of the D. suzukii genome databases 28 neuropeptide families, comprising more than 72 predicted peptides were identified. Using a combination of liquid chromatography and mass spectrometry of tissue extracts, 33 predicted peptides, representing 13 different peptide families were identified by their molecular masses and a total of 17 peptide sequences were confirmed by ion fragmentation.A comparison between the peptides and precursors of D. suzukii and D. melanogaster shows they are highly conserved, with differences only identified in the amino acid sequences of the peptides encoded in the FMRFamide, hugin and ecydysis triggering hormone precursors. All other peptides predicted and identified from D. suzukii appear to be identical to those previously characterized from D. melanogaster.Adipokinetic hormone was only identified in the corpus cardiacum, other peptides present included short neuropeptide F, a pyrokinin and myosuppressin, the latter of which was the only peptide identified from the crop nerve bundle. Peptides present in extracts of the brain and/or thoracicoabdominal ganglion included allatostatins, cardioacceleratory peptide 2b, corazonin, extended FMRFamides, pyrokinins, myoinihibitory peptides, neuropeptide-like precursor 1, SIFamide, short neuropeptide F, kinin, sulfakinins and tachykinin related peptides.

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Section: Introductionmentioning

confidence: 99%

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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“…Foregut extract from L. oleracea has been shown to rapidly degrade Manse-AS, with a half-life of approximately 5 min (Audsley et al, 2002a). Enzymes in the gut of the aphids A. pisum (Down et al, 2010) and M persicae (this study) also inactivate both Manse-AS and its dR analogue, but they appear to be less aggressive.…”

Section: Discussionmentioning

confidence: 68%

“…The responses to Manse-AS and dR 3 dR 5 Manse-AS were dependent on the dose ingested. Previous studies on Manse-AS and the analogue dR 3 dR 5 Manse-AS showed that they had dosedependent detrimental effects on the pea aphid A. pisum (Down et al, 2010) Effects of Manse-AS and an Analogue on M. persicae lower susceptibility to both Manse-AS and dR 3 dR 5 Manse-AS compared with A. pisum. Among aphid species, M. persicae is considered one of the most generalist, attacking many host plants (van Emden et al, 1969;Blackman and Eastop, 2000).…”

Section: Discussionmentioning

confidence: 92%

“…Although this study did not investigate the mechanism of action responsible for the effects exerted by the Manse-AS and dR 3 dR 5 Manse-AS analogue, a previous study on A. pisum suggested that these peptides may act by inhibiting gut motility or by inhibiting the dilator muscles of the pharynx pump (Down et al, 2010). Spontaneous …”

Section: Discussionmentioning

confidence: 92%

“…In vitro studies revealed that although there were enzymes in the gut of A. pisum capable of degrading both the peptides, it is likely that both Manse-AS and dR 3 dR 5 Manse-AS are able to traverse the aphid gut epithelium in an active form, and exert their effects possibly through the inhibition of gut motility or inhibition of the dilator muscles of the pharynx pump. The greater potency of the analogue was explained by its increased stability against enzymatic degradation (Down et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Manduca sexta allatostatin and an analogue on the peach‐potato aphid Myzus persicae (hemiptera: aphididae) and degradation by enzymes in the aphid gut

Matthews¹,

Down²,

Audsley³

2010

Arch Insect Biochem Physiol

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The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effects on mortality, growth, and fecundity compared with control insects. The analogue, δR³δR⁵Manse-AS, had an estimated LC₅₀ of 0.31 µg/µl diet and was more potent than Manse-AS (estimated LC₅₀ of 0.58 µg/µl diet). At a dose of 0.35 µg δR³δR⁵Manse-AS/µl diet, 76% of the aphids were dead after 6 days and all were dead after 10 days. In comparison, three times the dose of Manse-AS was required to achieve 74% mortality after 8 days and 98% mortality after 16 days. The degradation of both peptides by extracts prepared from the gut of M. persicae was investigated. The estimated half-life of Manse-AS, when incubated with the gut extract from M. persicae, was 31 min. Degradation was due to a cathepsin L-like cysteine protease, carboxypeptidase-like activity, endoprotease activity with glutamine specificity, pyroglutamate aminopeptidase activity, and possibly trypsin-like proteases. The half-life of the δR³δR⁵ Manse-AS analogue was enhanced (73 min) with the D-isomers of R appearing to prevent cleavage around the R residues by cathepsin L-like cysteine proteases or from trypsin-like proteases. The greater stability of the analogue may explain its increased potency in M. persicae. This work demonstrates the potential use of Manse-AS and analogues, with greater resistance to enzymatic attack, in aphid control strategies.

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Feeding-modulating neuropeptides and peptide hormones in insects

Nagata

Zhou

2019

Advances in Insect Physiology

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Effects of Manduca sexta allatostatin and an analog on the pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) and degradation by enzymes from the aphid gut

Cited by 22 publications

References 45 publications

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Effects of Manduca sexta allatostatin and an analogue on the peach‐potato aphid Myzus persicae (hemiptera: aphididae) and degradation by enzymes in the aphid gut

Feeding-modulating neuropeptides and peptide hormones in insects

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