Effects of manganese and Bacillus subtilis on the reproductive performance, egg quality, antioxidant capacity, and gut microbiota of breeding geese during laying period

Abstract

This experiment was conducted to investigate the effects of manganese ( Mn ) and Bacillus subtilis ( BS ) on the production performance, egg quality, antioxidant capacity, and gut microbiota of breeding geese during laying period. A total of 120 forty-six-week-old breeding geese (Wulong) were randomly assigned to 1 of 6 treatment diets formulated to supply 10, 20, and 30 mg/kg Mn with 5 × 10 9 CFU/kg or 2.5 × 10 9 CFU/kg BS for a 10-wk trial. Results showed that dietary supplementation with 20 and 30 mg/kg Mn could decrease the daily feed intake ( DFI ) of geese. Moreover, 30 mg/kg Mn significantly increased the laying rate. Besides, although Mn addition had no obvious effect on egg quality, 5 × 10 9 CFU/kg BS was found to elevate the hatching egg hatching rate and eggshell thickness. For the serum hormones, 30 mg/kg Mn promoted estradiol secretion, while 5 × 10 9 CFU/kg BS increased the level of follicle-stimulating hormone. Furthermore, 20 and 30 mg/kg Mn and 5 × 10 9 CFU/kg BS significantly enhanced the total antioxidant capacity by increasing the activity of total superoxide dismutases or decreasing the content of malondialdehyde. Dietary supplementation with 5 × 10 9 CFU/kg BS also increased the intestinal villus height and upregulated the abundance of Fusobacteria , Fusobacteriaceae , Fusobacterium , and Faecalibacterium in cecal content. In addition, 20 and 30 mg/kg Mn elevated the levels of Bacteroidetes , Bacteroidaceae , Bacteroides , and Ruminococcaceae but decreased Streptococcaceae . Importantly, an interaction effect was observed between Mn and BS on the DFI, egg mass, average egg size, and the abundance of Bacteroides as well as Faecalibacterium . In conclusion, dietary inclusion of Mn and BS could improve the production performance, egg quality, antioxidant capacity, intestinal structure, as well as gut microbiota. Supplementation of 30 mg/kg Mn and 5.0 × 10 9 CFU/kg BS provided the optimal effect.

Effects of zinc and Bacillus subtilis on the reproductive performance, egg quality, nutrient digestion, intestinal morphology, and serum antioxidant capacity of geese breeders

“…The addition of B. subtilis has been reported to play a positive effect on antioxidant activity of broilers ( Bai et al, 2018 ; Xu et al, 2021 ). Here, we found that 5 × 10 9 CFU/kg B. subtilis supplementation increased the T-AOC in serum of laying geese breeders, in agreement with our previous study ( Wang et al, 2020 ).…”

Grazing and Supplementation of Dietary Yeast Probiotics Shape the Gut Microbiota and Improve the Immunity of Black Fattening Goats (Capra hircus)

“…Studying the animal gut microbiota, Moeller et al (2018) reported that the composition of gut microbiota was different among animals. There are extremely significant differences among the diversity and richness of gut microbiota of horses ( Mshelia et al, 2018 ), rats ( Rolim et al, 2021 ), geese ( Wang et al, 2020 ), cows ( Uchiyama et al, 2020 ), and goats ( Liu et al, 2020 ). To date, there have been only few 16S ribosomal RNA (rRNA) gene sequencing technology-based studies, which have investigated the gut microbiota of small ruminants.…”

Effects of Dietary Bopu Powder Supplementation on Serum Antioxidant Capacity, Egg Quality, and Intestinal Microbiota of Laying Hens

“…Fusobacteriaceae could modulate the growth of other bacterial species through metabolic by-products, which was helpful for the maintenance of an overall microbial structure. Similarly, Wang et al (2020) found that dietary probiotic Bacillus subtilis supplementation increased the abundances of Fusobacteria phylum, Fusobacteriia class, Fusobacteriaceae family, and Fusobacterium genus and improved the performance of breeding geese during the laying period. Lachnospiraceae was involved in the production of SCFAs (butyrate, propionate, and acetate), which were important energy sources for intestinal epithelial cells (Rychlik, 2020).…”